Browsing by Author "Safwat G.M."

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    The biological activity of conocarpus erectus extracts and their applications as cytotoxic agents
    (Elsevier, 2018) Safwat G.M.; Hamed M.M.; Helmy A.T.; Faculty of Biotechnology; October University for Modern Sciences and Arts; Giza; Egypt; Theodor Bilharz Research Institute; Giza; Egypt
    Essential oils are found to have multiple active components which can show in vitro cytotoxic action against various cancerous cell lines. This study reports the in vitro cytotoxic effects of the essential oil from Conocarpus erectus (Combretaceae) growing wild in Egypt. Water-distilled essential oil of C. erectus was examined for its cytotoxic effects using a modified brine shrimp and MTT assays. Fresh leaves aerial part of C. erectus was subjected to hydro distillation using a Clevenger-type apparatusvolatile to obtain its volatile oil. Cytotoxicity of the essential oil was measured against HepG2 cancer cells and brine shrimps larva. The essential oil 50% cytotoxic concentrations were found to be 33�g/ml and 8.7�g/ml against brine shrimp and human liver carcinoma HepG2 cell line, respectively; thus the volatile oil displayed good cytotoxic action against the human tumor cell line. Moreover, C. erectus methanol extract was very effective; it exhibited cytotoxic activity against brine shrimp larva within IC50 value of 15�g/ml. The investigation from GC Mass, led to the identification of 12 constituents, representing 97.53% of the total oil, of which the major chemical constituents were identified by gas chromatography mass spectrometry as being rich in 3-(2,2 dimethylpropylid ene)bicyclo[3.3.1]nonane-2,4-dione (3) (67.12%), (decanoic acid derevatives (11) (7.77%), 22-tritetracontanone (12) (6.03%), 1-octanol, 2-butyl-(2) (5.51%) and oleic acid (6) (4.33%). This is the first report on anticancer potential and separation of essential oils from C. erectus. The findings of this study necessitate the need for further consideration of this essential oil in anti-neoplastic chemotherapy. � 2018, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.
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    Studies of the biological activity of Cassia fistula
    (SILAE (Italo-Latin American Society of Ethnomedicine), 2018) Safwat G.M.; Hamed M.M.; Moatamed S.A.; Faculty of Biotechnology; October University for Modern Sciences and Arts; Giza; Egypt; Medicinal Chemistry; Theodor Bilharz Research Institute; Giza; Egypt
    Cancer has been the most dreadful disease from ages till now. However, with the passage of time it been more understood its mechanism, types, some causing factors, and way of its cell duplication. By the progress of science there are now more than one available method for treatment, diagnosis or prevention of cancer. But this progress did not reach till now cheap, fast and effective specific targeting treatment for all types or even one type. For this aim, the field of searching for alternative natural compounds extracted from plants to replace those expensive non effective targeting compounds has been wider and advanced. One of familiar plant families is Leguminosae specifically class of Cassia fistula plant. This type of plant has been examined to ensure its biological activity and by detecting and analysis it show anticancer activity against liver cancer cell line; hepatocellular carcinoma HepG2. C. fistula volatile oil extract was able to inhibit proliferation for HepG2 cancer cell lines at 3.05 � 0.08�g/ml. The methanolic and oil extracts of Cassia fistula showed good brine shrimp larvicidal activity with lethality concentration (LC50) of 15 and 55 �g/mL, respectively. Finally using GC-MS, the essential oil compounds were identified, whereas 39 compounds produces and accounted for 90.46% of the total oil, the major compound was compound sulfurous acid; cyclohexyl-methyl octadecyl ester (21.62%). � 2018, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.