Browsing by Author "S. El-Halawany, Medhat"

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Inflammatory and Non-inflammatory Breast Cancer: A Potential Role for Detection of Multiple Viral DNAs in Disease Progression
(Springer US, 2016) El-Shinawi, Mohamed; Taha Mohamed, Hossam; Hesham Abdel-Fattah, Hadeer; Abdel Aziz Ibrahim, Sherif; S. El-Halawany, Medhat; Akram Nouh, M.; J. Schneider, Robert; Mostafa Mohamed, Mona
Background Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Multiple viral infections in IBC tissues were found to be associated with disease pathogenesis. Objective The aim of the present study was to correlate the incidence of viral DNA with breast cancer progression. Materials and Methods Overall, 135 women diagnosed with breast cancer were enrolled in this study. Using polymerase chain reaction and sequencing assays, we determined the incidence of human papillomavirus types 16 and 18 (HPV-16 and -18), human cytomegalovirus (HCMV), Epstein–Barr virus, human herpes simplex virus type 1 and 2, and human herpes virus type 8 (HHV-8) in breast carcinoma tissue biopsies. We also assessed the expression of the cell proliferation marker Ki-67 by immunohistochemistry in association with the incidence of viral DNA. Results HCMV and HPV-16 were the most detected viral DNAs in breast carcinoma tissues; however, the frequency of HCMV and HHV-8 DNA were significantly higher in IBC than non-IBC tissues. Moreover, the prevalence of multiple viral DNAs was higher in IBC than non-IBC tissues. The incidence of multiple viral DNAs positively correlates with tumor size and number of metastatic lymph nodes in both non-IBC and IBC patients. The expression of Ki-67 was found to be significantly higher in both non-IBC and IBC tissues in which multiple viral DNAs were detected. Conclusions The incidence of multiple viral DNAs in IBC tissues was higher compared with non-IBC tissues. The present results suggest the possibility of a functional relationship between the presence of multiple viral DNAs and disease pathogenesis.
Inflammatory breast cancer: Mixed viral infections within carcinoma tissues and the expression of Ki-67 proliferation marker.
(American Society of Clinical Oncology, 2017) taha Mohamed, Hossam; Hesham Abdel Fattah, Hadeer; El-Shinawi, Mohamed; Abdelaziz Ibrahim, Sherif; S. El-Halawany, Medhat; El Ghazaly, Hesham; Schneider, Robert; Mohamed, Mona
Background: Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Our previous results showed that IBC carcinoma tissues possess mixed human cytomegalovirus genotypes than non-IBC carcinoma tissues. However, the role of viral infection in breast cancer is poorly understood. Methods: We enrolled 135 women diagnosed breast cancer (91 Non-IBC and 44 IBC). The incidence of different viral DNA (Herpes viruses and HPV) was performed using nested and multiplex PCR and DNA sequencing. The expression of Ki-67 proliferation index was assessed by immunohistochemistry. Results: DNA of HCMV and HPV-16 were the most detected in breast tissues of both IBC and non-IBC patients. However, as a single infection the incidence of HCMV-DNA and HHV-8 DNA were significantly higher in carcinoma tissues of IBC in comparison with non-IBC (p = 0.035, p= 0.039, respectively). Moreover, the prevalence of mixed infection of different viral DNA was higher in IBC than non-IBC carcinoma tissues (P= 0.003). HCMV and HPV-16 were the dominant mixed infection in both non-IBC and IBC tissues. Interestingly, although no significant difference in expression of Ki67 has been detected in tissues of IBC and non-IBC, we found that Ki-67 was significantly higher in mixed than single viral infected tissues of both non-IBC and IBC (p = 0.04 and p = 0.03 respectively). Conclusions: The incidence of mixed viral DNA detected in carcinoma tissues of IBC is higher than non-IBC. Moreover, mixed viral DNA is positively correlated with upregulation of Ki67 expression in breast carcinoma tissues.