Browsing by Author "S Hassan, Osama"

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    Quantification of the gene expression of bell peppers (Capsicum annuum) ripening gene(s) using real -time PCR
    (Academic Journals (Kenya), 2014) S Hassan, Osama; Badie, Fatma; Safwat, Gehan
    Fruits can be divided into two groups according to the regulatory mechanisms underlying their ripening process. The two ripening processes are climacteric and non-climacteric process; bell peppers are part of the non-climacteric plant groups. Bell peppers are members of the Solanacaea family. The Solanacaea family is best known for its fruits around the world. Today’s main focus is targeted to fruit ripening, in an attempt to increase the fruit’s shelf life. Many genes have been linked to the maturation of the fruit such as in Arabidopsis, the genes found were elongation factor-1α (LeEF-1A), expansin protein (leEXP1) and ripening inhibitor (RIN). This research focused on discovering similar genes that may play an important role in the ripening of peppers. Real-time PCR was performed on the cDNA of the green bell pepper fruit during its stage of development in order to detect and identify the expression pattern of the gene. Through the comparison of the gene expression found in bell pepper and the pods of Arabidopsis as model to dicotyledonous plant, some variations have been detected.
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    Universal and Specific 16S-23Sr RNA PCR Primers forIdentification of Phytoplasma associated with sesame in Egypt
    (International Journal of Advanced Research in Biological Sciences, 2017) A Youssef, Sahar; Sayed, Yasmin; S Hassan, Osama; Safwat, Gehan; Shalaby, AA
    Sesame (Sesamum indicumL.), familyPedaliaceaeis one of the most ancient cultivated oilseed cropsamong the edible annualgroup in Egypt and all over the world. Phytoplasmas are pathogens of many plant species throughout the world including sesamecausing a large variety of symptoms ranged from mild yellowing to death of infected plants.Symptomaticsamples includinggreen leaf-like floral organs,virescence, phyllody and proliferationwere collected from infected sesame field in GizaGovernorate.This research was undertaken to develop a diagnostic tool to identify phytoplasmas recently discovered infectingsesame in Egypt, and to classify them according to their phylogenetic group. Direct and nested PCR primers of 16S-23SrRNAgene, paired with phytoplasma universal primer followed by strain-specific PCR primers were used in this study. Spacer Region(SR) primers were employed for identification of Phytoplasma group associated with sesame symptoms. Results showed thepresences of mixed infections of phytoplasma in the tested sesame samples which collected from the field with different typeofPhytoplasma like symptoms. Results also indicated thatpolymerase chain reaction with primers from sequencing of 16S-32SrRNA and from SR opened new paths for research on phytoplasma identification and classification. Nested PCR has been appliedto overcome problems related to sensitivity of phytoplasma detection, although this approach is more time consuming and subjectto template. Unfortunately, nested-PCR also meets some difficulties: unspecific bands, false positives or negatives caused byDNA and contaminationof single or nested PCR. Therefore, confirmation of PCR results by using different primer paircombinations (generic and group-specific) seems to be the way for correct phytoplasma identification in the examined sesamesamples