Browsing by Author "Nematallah K.A."

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    Botanical and genetic characteristics of Lobularia libyca (viv). C.F.W. Meissn. (Brassicaceae)
    (International Journal of Pharmacognosy and Phytochemical Research, 2014) Zaghloul S.S.; Nematallah K.A.; Al-Gendy A.A.; Ayoub N.; Pharmacognosy Department; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Zagazig University; Al-Sharkiya; Egypt; Pharmacognosy Department; Ain-Shams University; Cairo; Egypt; Pharmacognosy Department; BUE University; Cairo; Egypt
    Lobularia libyca (viv). C.F.W. Meissn. is an annual herbaceous desert plant native to North Africa belonging to Family Brassicaceae (Cruciferae). Study of the botanical features was carried out for the root, the young and old stems, leaf, flower and seed of the plant. L. libyca was characterized by the presence of non-glandular branched unicellular two armed hair in the stem, leaf and flower while the root shows narrow cork and xylem occupies about 80% of the diameter of the root. The flower is characterized by hairy sepals and spherical pollen grains with three germ pores and three germ furrows. The pericarp is characterized by hairy epicarp and presence of fibrosclerides as innermost layer of the mesocarp. The septum shows pitted lignified parenchyma. The seed shows accumbent embryo and the testa is characterized by the presence of pigment layer with reddish brown content. The DNA of the plant was extracted from leaf samples and analyzed using eleven random decamer primers. A total of 89 random amplified polymorphic DNA (RAPD) markers were identified. Both the botanical study and the DNA fingerprint helped in the identification of the plant.
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    Glucosinolates profile, volatile constituents, antimicrobial, and cytotoxic activities of Lobularia libyca
    (Institute of Electrical and Electronics Engineers Inc., 2016) Al-Gendy A.A.; Nematallah K.A.; Zaghloul S.S.; Ayoub N.A.; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Faculty of Pharmacy; Zagazig University; Zagazig; Egypt; Faculty of Pharmacy; British University in Egypt; Cairo; Egypt; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; Faculty of Medicine; Umm Al-Qura University; Makkah; Saudi Arabia
    Context: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0�160 ?g/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01�100 ?g/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC = 64 and 82 ?g/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 ?g/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. � 2016 Informa UK Limited, trading as Taylor & Francis Group.