Browsing by Author "Nagy D.M."

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    Spectrofluorimetric determination of certain biologically active phenothiazines in commercial dosage forms and human plasma
    (2013) Mohamed A.-M.I.; Abdelmageed O.H.; Salem H.; Nagy D.M.; Omar M.A.; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; Assiut University; Assiut; Egypt; Pharmaceutical Chemistry Department; Faculty of Pharmacy; King Abdulaziz University; Jeddah; Saudi Arabia; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; MSA University; Cairo; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Minia University; Minia; Egypt
    A validated simple and sensitive spectrofluorimetric method was developed for the determination of chlorpromazine hydrochloride, promethazine hydrochloride, trifluperazine hydrochloride, thioridazine hydrochloride, perazine maleate and oxomemazine. The method was based on condensation of malonic acid/acetic anhydride (MAA) under the catalytic effect of the tertiary amine moiety of the studied phenothiazines to provide a deep yellow to brown colour with green florescence. Relative fluorescence intensity of the products was measured at ?exc 398 nm and ?em 432 nm. Different variables affecting the reaction were studied and optimized. The method was successfully applied for the determination of the studied drugs in commercial dosage forms. The lower detection limits allowed the application of this method for the determination of the compounds in plasma as an example of a biological fluid. In addition, the method was considered specific for the determination of tertiary amines in the presence of primary and secondary amines; as a result, it was deemed suitable for the determination of the cited drugs in the presence of their degradation products resulting from N-dealkylation or oxidation of the corresponding sulphoxides or sulphones. Copyright � 2012 John Wiley & Sons, Ltd.