Browsing by Author "Moussa H.S."

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Clinical and laboratory profile of urinary tract infections associated with extended spectrum-lactamase producing Escherichia coli and Klebsiella pneumoniae
(International Journal of Green Economics, 2016) Al Yousef S.A.; Younis S.; Farrag E.; Moussa H.S.; Bayoumi F.S.; Ali A.M.; Department of Medical Laboratory Technology; College of Applied Medical Sciences; University of Hafr Al Batin; Hafr Al Batin; Saudi Arabia; Faculty of Women for Art; Science and Education; Ain Shams University; Egypt; Microbiology Department; Faculty of Pharmacy; MSA University; Cairo; Egypt
Background. Urinary tract infection (UTI) is mainly due to invasion of the urethra, bladder or kidneys by pathogens. The emergence of extended spectrum �-lactamases (ESBL) is responsible for frequently observed empirical therapy failures. Objectives. To study the clinical and laboratory characteristics of UTIs caused by ESBL producing Escherichia coli (E. coli) and Klebsiella pneumonia (K. pneumonia). Methods. A cross-sectional clinical and laboratory study was performed at King Khalid Hospital, Hafr Al Batin, Saudi Arabia between March 2014 to October 2015. A total of 908 urine samples from suspected UTI patients was collected. Samples were isolated on Cysteine Electrolyte-Deficient (CLED) agar. Positive cultures were identified and tested for antimicrobial susceptibility by MicroScan WalkAway-96 SI System, and then ESBL was confirmed by double disc synergy test (DDST) and phenotypic confirmatory disc diffusion test (PCDDT). Results. A total of 680 samples (288 males and 392 females) were culture positive. 520 samples (76.5%)of E. Coli were found and 160 samples of K. pneumonia were identified (23.5%). ESBL testing showed 296 (218 E. coli and 78 K. pneumonia) samples of positive isolates. Non-ESBL isolates showed highest resistance to ampicillin followed by Mezocillin and Trimethoprim-Sulphamethoxazole- which are usually recommended as the initial treatment of UTI-while ESBL isolates showed resistance to third generation cephalosporin along with Ampicillin and Trimethoprim-Sulphamethoxazole. In this study, four significant risk factors for ESBL infection such as diabetes, recurrent UTI, previous use of antibiotics and previous hospitalization were found. Conclusion. Identifying the risk factors and antibiotic susceptibility patterns associated with ESBL producing E. coli and K. pneumonia is a useful guide for treatment strategy and control of ESBL UTI. 2016 by the Association of Clinical Scientists, Inc.
Evaluation of genexpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis
(Saudi Arabian Armed Forces Hospital, 2016) Moussa H.S.; Bayoumi F.S.; Ali A.M.; Botany Department; Faculty of Women for Art; Science; and Education; Ain Shams University; Cairo; Egypt; Microbiology Department; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt; Department of Medical Laboratory Technology; College of Applied Medical Science; University of Dammam; Hafr Al Batin; Saudi Arabia
Objectives: To evaluate the performance of GeneXpert MTB/RIF assay for direct diagnosis of pulmonary tuberculosis (PTB). Methods: This is a cross-sectional study conducted between October 2013 and February 2016 at Abbassaia Chest Hospital and Ain Shams University Hospital, Cairo, Egypt. Inclusion criteria were adults between 18 and 60 years with suspected PTB and classified into 5 clinical categories based on their clinical, radiological, and laboratory findings: confirmed TB, probable TB, possible TB, unlikely TB, and not TB. Two sputum samples from each participant were analyzed by GX and the results were compared by conventional culture. Results: In total, 218 participants were enrolled: 71 had confirmed TB; 112, highly probable TB; 20, probable TB; 10, unlikely TB; and 5, no TB. The sensitivity and specificity of the GX assay were 93% and 98.3% respectively. GeneXpert was positive in 93% of confirmed TB and 2.2% of probable TB cases. Conclusions: GeneXpert is a rapid and promising technique with good sensitivity (93%) and specificity (98.3%), but it cannot be used as a standalone PTB diagnostic tool. There is a need for more GX evaluation studies in countries with low TB incidence. � 2016, Saudi Arabian Armed Forces Hospital. All rights reserved.
Gene Xpert for direct detection of Mycobacterium Tuberculosis in stool specimens from children with presumptive pulmonary tuberculosis
(Association of Clinical Scientists, 2016) Moussa H.S.; Bayoumi F.S.; Mohamed A.M.A.; Faculty of Women for Art; Science and Education; Ain Shams University; Cairo; Egypt; Microbiology Department; Faculty of Pharmacy; MSA University; Cairo; Egypt; Department of Immunogenetics; National Research Centre; Giza; Egypt; Egyptian Company of Serum and Vaccines; Giza; Egypt
Background. Gene Xpert(GX) is a novel real time polymerase chain reaction (RT-PCR) assay which was endorsed by the World Health Organization (WHO) in 2011 for tuberculosis (TB) diagnosis and susceptibility to refampicin(RIF). Objective. To evaluate GX for direct diagnosis of TB in stool samples from children with suspected pulmonary Tuberculosis (PTB). Methods. Children older than one year and younger than 16 years with presumptive PTB were enrolled and classified to five clinical categories based on clinical, radiological, and laboratory findings: Confirmed TB, probable TB, possible TB, Unlikely TB, and not TB. Two stool samples were collected from each child and tested for the presence of Mycobacterium tuberculosis (MTB) by GX and the obtained results were compared to Lowenstien-Jensen (LJ) culture as a gold standard. Results. In total, 115 children were enrolled. 36 had been confirmed with TB, 61 probably TB, 10 possible TB, 5 unlikely TB, and 3 not TB. GX had a sensitivity of 83.33 and 80.56 % and specificity of 98.73 and 99.36 % by patients and samples respectively. GX was positive in 83.3% of confirmed TB as well as 1.6 and 0.8% of probable TB cases by patients and samples respectively. Conclusions. GX provided timely results with quit acceptable sensitivity and good specificity compared to LJ culture. In this study, sensitivity calculations take into account only children with confirmed TB. GX could not detect TB in children with probable TB, so it should not be used alone for TB diagnosis. Further studies for GX stool protocol optimization and assessment is required. � 2016 by the Association of Clinical Scientists, Inc.