Browsing by Author "Motawi T.M.K."

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Serum human leukocyte antigen-G and soluble interleukin 2 receptor levels in acute lymphoblastic leukemic pediatric patients
(Asian Pacific Organization for Cancer Prevention, 2012) Motawi T.M.K.; Zakhary N.I.; Salman T.M.; Tadros S.A.; Biochemistry Department; Faculty of Pharmacy; Egypt; Cancer Biology Department; National Cancer Institute; Egypt; Cairo University; Egypt; Al-Azhar University; Egypt; MSA University; Egypt
Aims and Background: Human leukocyte antigen-G and interleukin-2 receptor play pivotal roles in the proliferation of lymphocytes, and thus generation of immune responses. Their overexpression has been evidenced in different malignant hematopoietic diseases. This study aimed to validate serum soluble human leukocyte antigen-G (sHLA-G) and serum soluble interleukin-2 receptor (sIL-2R) as an additional tool for the diagnosis and follow up of acute lymphoblastic leukemia (ALL). Subjects and Methods: Both markers were determined by ELISA in the serum of 33 ALL pediatric patients before treatment and after intensification phase of chemotherapy as well as in the serum of 14 healthy donors that were selected as a control group. Results: ALL patients showed abnormal CBC and high serum lactate dehydrogenase, which were improved after chemotherapy. Also, there was a non-significant increase in serum sHLA-G in ALL patients compared with the control group. However, after chemotherapy, sHLA-G was increased significantly compared with before treatment. On the other hand, serum sIL-2R in ALL patients was increased significantly compared with the control group. After chemotherapy, sIL-2R decreased significantly compared with before treatment. Conclusions: From these results it could be suggested that measurement of serum sHLA-G might be helpful in diagnosis of ALL, while sIL-2R might be useful in diagnosis and follow-up of ALL in pediatric patients.
Significance of Serum Survivin and -31G/C Gene Polymorphism in the Early Diagnosis of Breast Cancer in Egypt
(Elsevier Inc., 2019) Motawi T.M.K.; Zakhary N.I.; Darwish H.A.; Abdalla H.M.; Tadros S.A.; Department of Biochemistry; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Cancer Biology; National Cancer Institute; Cairo University; Cairo; Egypt; Board of Trustees; The British University in Egypt (BUE); Cairo; Egypt; Department of Pharmacology; Toxicology; and Biochemistry; Faculty of Pharmaceutical Sciences and Pharmaceutical Industries; Future University; Cairo; Egypt; Department of Surgical Oncology; National Cancer Institute; Cairo University; Cairo; Egypt; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); October; Egypt
The majority of breast cancer cases are discovered in later disease stages, thus affecting survival rate. We studied the impact of survivin -31 G/C single nucleotide polymorphism and its serum level alteration. Minor allele C and the GC + CC genotype occurred more frequently in breast cancer patients; further, increased breast cancer risk with associated with elevated serum survivin level. These data could help in early diagnosis and understanding of the pathogenesis of breast cancer. 2019 Elsevier Inc.Background: Breast cancer is one of the most relevant malignancies among women. Molecular abnormalities in promotor region of survivin gene may account for overexpression of survivin and increased breast cancer risk. This study aimed to explore the potential association between survivin promotor gene -31G/C single nucleotide polymorphism (rs9904341) and its serum level alteration on one hand, and the risk of breast cancer in Egyptian patients on the other hand. It also aimed to assess the usefulness of survivin as an early noninvasive diagnostic biomarker and in breast cancer staging. Patients and Methods: A total of 135 patients with physically and pathologically confirmed breast cancer and 40 unrelated control subjects as well as 40 patients with benign breast mass were recruited from the early detection unit at National Cancer Institute, Cairo University. Genotyping was performed using allelic discrimination probes by real-time quantitative PCR and serum survivin by enzyme-linked immunosorbent assay. Results: The minor allele C of -31G/C survivin single nucleotide polymorphism was more frequent in breast cancer patients (19.3%) compared to the control group (7.5%). Furthermore, subjects with the GC + CC genotype were at increased risk of breast cancer compared to the GG genotype of the control group and also the benign group. Moreover, those patients exhibited higher serum levels of survivin compared to GG genotype. There was also significant elevation of serum survivin in different breast cancer stages. Conclusion: Genetic variation in -31G/C of the survivin gene may contribute to the disposition of breast cancer in the Egyptian population. Serum survivin alteration played a pivotal role in the pathogenesis of breast cancer. 2019 Elsevier Inc.
Study of microRNAs-21/221 as potential breast cancer biomarkers in Egyptian women
(Elsevier B.V., 2016) Motawi T.M.K.; Sadik N.A.H.; Shaker O.G.; El Masry M.R.; Mohareb F.; Biochemistry Department; Faculty of Pharmacy; Cairo University; Kasr El-Einy; Cairo; Egypt; Medical Biochemistry and Molecular Biology Department; Faculty of Medicine; Cairo University; Egypt; Biochemistry Department; Faculty of Dentistry; October University for Modern Sciences & Arts (MSA); Giza; Egypt; The Bioinformatics Group; School of Energy; Environment and AgriFood; Cranfield University; Bedford; MK43 0AL; United Kingdom
microRNAs (miRNAs) play an important role in cancer prognosis. They are small molecules, approximately 17�25 nucleotides in length, and their high stability in human serum supports their use as novel diagnostic biomarkers of cancer and other pathological conditions. In this study, we analyzed the expression patterns of miR-21 and miR-221 in the serum from a total of 100 Egyptian female subjects with breast cancer, fibroadenoma, and healthy control subjects. Using microarray-based expression profiling followed by real-time polymerase chain reaction validation, we compared the levels of the two circulating miRNAs in the serum of patients with breast cancer (n=50), fibroadenoma (n=25), and healthy controls (n=25). The miRNA SNORD68 was chosen as the housekeeping endogenous control. We found that the serum levels of miR-21 and miR-221 were significantly overexpressed in breast cancer patients compared to normal controls and fibroadenoma patients. Receiver Operating Characteristic (ROC) curve analysis revealed that miR-21 has greater potential in discriminating between breast cancer patients and the control group, while miR-221 has greater potential in discriminating between breast cancer and fibroadenoma patients. Classification models using k-Nearest Neighbor (kNN), Nave Bayes (NB), and Random Forests (RF) were developed using expression levels of both miR-21 and miR-221. Best classification performance was achieved by NB Classification models, reaching 91% of correct classification. Furthermore, relative miR-221 expression was associated with histological tumor grades. Therefore, it may be concluded that both miR-21 and miR-221 can be used to differentiate between breast cancer patients and healthy controls, but that the diagnostic accuracy of serum miR-21 is superior to miR-221 for breast cancer prediction. miR-221 has more diagnostic power in discriminating between breast cancer and fibroadenoma patients. The overexpression of miR-221 has been associated with the breast cancer grade. We also demonstrated that the combined expression of miR-21 and miR-221can be successfully applied as breast cancer biomarkers. 2016 Elsevier B.V.
Visfatin -948 G/T and resistin -420 C/G polymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseases
(National Research Council of Canada, 2014) Motawi T.M.K.; Shaker O.G.; El-Sawalhi M.M.; Abdel-Nasser Z.M.; Biochemistry Department; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Medical Biochemistry and Molecular Biology Department; Faculty of Medicine; Cairo University; Cairo; Egypt; Biochemistry Department; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt
Diabetes mellitus is one of the main threats to human health in the 21st century. Visfatin/Nampt and resistin are novel adipokines that have been implicated in the pathogenesis of type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) complication. Several genetic studies have shown inconsistent results regarding association of visfatin/Nampt gene (NAMPT) and resistin gene (RETN) polymorphisms with T2DM and CVD complications. Here, we investigate whether NAMPT -948G/T and RETN -420C/G polymorphisms are associated with T2DM, its CVD complications, and serum adipokines levels in 90 Egyptian diabetic patients (44 without CVD and 46 with CVD) along with 60 healthy control subjects. Higher frequencies of NAMPT -948G/G and RETN -420G/G were observed among T2DM patients compared with controls. Furthermore, the frequencies of these genotypes were significantly higher in T2DM patients with CVD than those without CVD. Both NAMPT -948G/G and RETN -420G/G genotypes and G alleles were significantly associated with T2DM and CVD in Egyptian diabetic patients. Moreover, serum visfatin/Nampt and resistin levels were markedly elevated in T2DM patients, with the highest values observed in G/G genotypes among T2DM patients with CVD. In addition, positive correlations were observed between plasma adipokines levels and CVD risk factors. In conclusion, our data suggests that genetic variations in NAMPT -948G/T and RETN -420C/G may contribute to the disposition for T2DM and its CVD complications in Egyptian patients. However, further studies with greater sample size should be performed to verify these results. 2014 Published by NRC Research Press.