Browsing by Author "Mostafa E.S."

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    Antioxidant activity and molecular docking study of Erythrin neillii polyphenolics
    (Elsevier B.V., 2019) Gabr S.K.; Bakr R.O.; Mostafa E.S.; El-Fishawy A.M.; El-Alfy T.S.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt
    Species of genus Erythrina have a great contribution in folk medicine; various species are utilized as a tranquilizer, to treat insomnia, inflammation and colic. Besides, Erythrina species have reported antioxidant, hepatoprotective and anxiolytic activities. Erythrina neillii is a hybrid obtained through a cross between E. herbacea L. and E. humeana Spreng. It has not been well-studied for its chemical or biological profile; therefore it represents an interesting field of study. In this study, seven phenolic compounds; two hydrolysable tannins (1,3), one phenolic acid (2) and four known flavonoids (4�7) were isolated and characterized for the first time in E neillii and Erythrina genus except for vitexin (7). Isolated compounds were assessed for their antioxidant activities using ORAC assay. 2?-O-galloyl orientin (6) exhibited the highest activity followed by 2?-O-galloyl vitexin (5). Flexible molecular docking on heme oxygenase, an important stress protein that is involved in cellular protection, antioxidant and anti-inflammatory activities, justified the antioxidant activity of the isolated compounds. The best scoring was observed with 2?-O-galloyl orientin forming four binding interactions with residues, Arg 136 (two interactions), Met34 and Gly139. Erythrina neillii offered powerful and available antioxidant beside significantly active phytoconstituents. 2018 South African Association of Botanists
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    Chemical profile of two jasminum sambac L. (AIT) cultivars cultivated in egypt-their mediated silver nanoparticles synthesis and selective cytotoxicity
    (Innovare Academics Sciences Pvt. Ltd, 2019) El-Hawary S.S.; El-Hefnawy H.M.; Osman S.M.; Mostafa E.S.; Mokhtar F.A.; El-Raey M.A.; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6thOctober City; Giza; Egypt; Phytochemistry and Plant Systematic Department; National Research Centre; Dokki; Cairo; Egypt
    Objective: Evaluation of two Jasminum sambac L. (Ait) cultivars; Arabian Nights (JSA) and Grand Duke of Tuscany (JSG) ethanolic leaves extracts as reducing agents for the green synthesis of silver nanoparticles (AgNPs) and evaluation of their cytotoxicity against MCF-7 breast cancer and 5637 bladder cancer cell lines and chemical profiling of the two cultivars. Methods: The synthesis of silver nanoparticles (AgNPs) by the two cultivars and characterization of AgNPs by ultraviolet (UV)�visible spectroscopy, Transmission electron microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR). Additionally, the use of The high-performance liquid chromatography coupled with photodiode array-mass-mass-spectroscopy (HPLC-PDA-MS/MS) for chemical profiling of both cultivars and evaluation of total leaves extracts and corresponding nanoparticles towards MCF-7 and 5637 cell lines compared to aneuploidy immortal keratinocyte (Ha Cat) normal cells by neutral cell assay. Results: The green synthesized AgNPs (of an average size range of 8.83 and 11.24 nm for JSA and JSG, respectively) exhibited cytotoxicity against MCF-7 and 5637 cell lines. The IC50 was determined for each total extract JSA (15.29�2.16 ?g/ml) and JSG (20.28�1.20 ?g/ml) and corresponding AgNPs 17.32�2.22 ?g/ml and 6.32�1.01?g/ml for JSA and JSG, respectively. The IC50 of JSA and JSG against 5637 bladder cancer cell line were 13.76�1.11 ?g/ml and 50.69�3.75 ?g/ml, while the corresponding AgNPs showed IC50 of 5.54�0.88 ?g/ml and 27.89�2.84 ?g/ml, respectively. The HPLC-PDA-MS/MS allowed the identification of 59 compounds; 10 simple phenols, 17 flavonoids; quercetin and kaempferol glycosides, 2 lignans, and 30 secoiridoids; oleuropein, molihauside, and sambacoside. Conclusion: This study proved that JSA is an excellent source for the synthesis of AgNPs with optimum characters and enhanced activities toward MCF-7 and 5637 cell lines in correlation to identified compounds. � 2019 The Authors.
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    Polyphenols in Ammania auriculata: Structures, antioxidative activity and cytotoxicity
    (Govi-Verlag Pharmazeutischer Verlag GmbH, 2014) Nawwar M.A.; Youb N.A.; El-Raey M.A.; Zaghloul S.S.; Hashem A.M.; Mostafa E.S.; Eldahshan O.; Werner V.; Becker A.; Haertel B.; Lindequist U.; Linscheid M.W.; Department of Phytochemistry and Plant Systematics; National Research Center; Dokki Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; October University for Modern Sciences and Arts; 6th October City; Egypt; Institute of Pharmacy; Pharmaceutical Biology; Ernst-Moritz-Arndt-Universit�t; Greifswald; Mecklenburg-Vorpommern; Germany; Department of Chemistry; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt -University; Berlin; Germany
    Chemical and biological investigations of the extract of Ammania auriculata (Lytheraceae) resulted in the identification of eight polyphenols (1 - 8) for the first time from this plant, including the gallotannin, 2,3,6-tri-O-galloyl-(?,?)-4C1-glucopyranose (8), for which 1D and 2D-NMR spectra were recorded and assigned for the first time. The structures of all isolates (1 - 8) were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HR-ESIMS as well. All of the isolated compounds were evaluated for their antioxidant activities, determined by the DPPH and ORAC methods and for their cytotoxicity against the keratinocyte cell line HaCaT using the neutral red assay (NRU) and cell cycle analysis. Compounds 1, 3, 4, 5, and 6 significantly inhibited reactive oxygen species production with ED50 values between 3.22 and 9.79 ?g/ml. Compounds 1, 3, 4, and 5 showed cytotoxic activity against HaCaT cells with IC50 values between 30.7 and 84.1 ?g/ml. The new galloyl glucose (8) was found not cytotoxic. Ellagitannins, 2,3-hexahydroxy-(?/?)-glucopyranose (1) and 1-O-galloyl 2,3-hexahydroxy-(?)-glucopyranose (5) possess remarkable antioxidative and comparably weak cytotoxic activity.