Browsing by Author "Mostafa, Dalia A"

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    Antimicrobial potential of Mentha Spp. essential oils as raw and loaded solid lipid nanoparticles against dental caries
    (rjpt, 2020-09) Mostafa, Dalia A; Bayoumi, Faten S.; Taher, Heba M.; Abdelmonem, Basma H.; Eissa, Tarek F.
    This study evaluates the antimicrobial potential of Mentha spp. (M.spicata L.,M. xpiperita L. and M. pulegium L.) essential oils as raw and loaded solid lipid nanoparticles (SLNs)against dental caries. Design: Essentials oils extraction from fresh aerial parts of Mentha spp. was carried out using hydro distillation technique. Solid lipid nanoparticles of Mentha essential oil were prepared by w/o/w type double emulsification method. The antimicrobial activity of both pure Mentha spp. essential oils and Mentha spp. SLNs was determined against bacteria presented in saliva collected from 12 patients using agar diffusion assay. Results: Mentha spp. essential oils loaded solid lipid nanoparticles (MSLNs) were spherical shaped with sizes ranged from 111 to 202 nm and with PDI from 0.43 to 0.76, EE% between 85 and 88, and ZP of −11.8 to -40 mV. Antimicrobial results showed that MSLNs exhibited higher in vitro antimicrobial activity than pure Mentha spp. essential oil. Particularly, with an inhibition zone of 20 mm. These both MSLNs were even more active than the reference compound novobiocin. Conclusion: Our findings demonstrate that Mentha spp. essential oils as a nanostructure increase the efficiency of these natural products as antibacterial agents against caries.
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    Enhanced In Vivo Wound Healing Efficacy of a Novel Hydrogel Loaded with Copper (II) Schiff Base Quinoline Complex (CuSQ) Solid Lipid Nanoparticles
    (Multidisciplinary Digital Publishing Institute (MDPI), 2022-08-08) Abou El-ezz, Doaa; Abdel-Rahman, Laila H; Al-Farhan, Badriah Saad; Mostafa, Dalia A; Ayad, Eman G; Basha, Maram T; Abdelaziz, Mahmoud; Abdalla, Ehab M
    Wound dressings created using nanotechnology are known as suitable substrates to speed up the healing of both acute and chronic wounds. Therapeutic substances can be delivered using these materials. In this study, a hydrogel loaded with Cu (II) Schiff base 8-hydroxy quinoline complex (CuSQ) solid lipid nanoparticles (SLN) was formulated to investigate its wound healing potential in an excision wound healing model in rats. The CuSQ SLN were spherical shaped with sizes ranging from 111 to 202 nm and a polydispersity index (PDI) ranging from 0.43 to 0.76, encapsulation efficiency (EE) % between 85 and 88, and zeta potential (ZP) of −11.8 to −40 mV. The formulated hydrogel showed good homogeneity, good stability, and a pH of 6.4 which indicates no skin irritation and had no cytotoxicity on the human skin fibroblast (HSF) cell line. In the in vivo study, animals were placed in five groups: control, standard, plain hydrogel, low dose, and high dose of CuSQ hydrogel. Both doses of CuSQ showed significantly faster healing rates compared to standard and control rats. In addition, the histopathology study showed more collagen, improved angiogenesis, and intact re-epithelization with less inflammation. A significant increase in transforming growth factor-beta1 (TGF-β1) level and increased immune expression of vascular endothelial growth factor (VEGF) by CuSQ treatment validates its role in collagen synthesis, proliferation of fibroblasts and enhancement of angiogenesis. Matrix metalloproteinase-9 (MMP-9) was found to be significantly reduced after CuSQ treatment. Immunohistochemistry of tumor necrosis factor alpha (TNF-α) revealed a marked decrease in inflammation. Thus, we concluded that CuSQ would be a beneficial drug for cutaneous wound healing since it effectively accelerated wound healing through regulation of various cytokines and growth factors.
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    A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare
    (MDPI, 03/12/2021) Mostafa, Eman S; Maher, Ahmed; Mostafa, Dalia A; Gad, Sameh S; Nawwar, Mahmoud A.M; Swilam, Noha
    MDPI Open Access Journals zoom_out_map search menu Journals Antioxidants Volume 10 Issue 3 10.3390/antiox10030436 antioxidants-logo Submit to this Journal Review for this Journal Edit a Special Issue ► Article Menu Open AccessArticle A Unique Acylated Flavonol Glycoside from Prunus persica (L.) var. Florida Prince: A New Solid Lipid Nanoparticle Cosmeceutical Formulation for Skincare by Eman S. Mostafa 1,*,Ahmed Maher 2,*OrcID,Dalia A. Mostafa 3OrcID,Sameh S. Gad 4OrcID,Mahmoud A.M. Nawwar 5 andNoha Swilam 6OrcID 1 Department of Pharmacognosy, Faculty of Pharmacy, October University of Modern Sciences and Arts (MSA), Giza 12451, Egypt 2 Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt 3 Department of Pharmaceutics, Faculty of Pharmacy, October University of Modern Sciences and Arts (MSA), Giza 12451, Egypt 4 Department of Pharmacology, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Giza 12451, Egypt 5 National Research Centre, Department of Phytochemistry, Dokki, Cairo 12622, Egypt 6 Department of Pharmacognosy, Faculty of Pharmacy, The British University in Egypt (BUE), Cairo 11837, Egypt * Authors to whom correspondence should be addressed. Academic Editors: Alfredo Aires and Yong Chool Boo Antioxidants 2021, 10(3), 436; https://doi.org/10.3390/antiox10030436 Received: 24 February 2021 / Revised: 6 March 2021 / Accepted: 8 March 2021 / Published: 12 March 2021 (This article belongs to the Special Issue Phenolics as Antioxidant Agents) View Full-Text Download PDF Browse Figures Citation Export Abstract Polyphenols are known dietary antioxidants. They have recently attracted considerable interest in uses to prevent skin aging and hyperpigmentation resulting from solar UV-irradiation. Prunus persica (L.) leaves are considered by-products and were reported to have a remarkable antioxidant activity due to their high content of polyphenols. This study aimed at the development of a cosmeceutical anti-aging and skin whitening cream preparation using ethanol leaves extract of Prunus persica (L.) (PPEE) loaded in solid lipid nanoparticles (SLNs) to enhance the skin delivery. Chemical investigation of PPEE showed significantly high total phenolic and flavonoids content with notable antioxidant activities (DPPH, ABTS, and β-carotene assays). A unique acylated kaempferol glycoside with a rare structure, kaempferol 3-O-β-4C1-(6″-O-3,4-dihydroxyphenylacetyl glucopyranoside) (KDPAG) was isolated for the first time and its structure fully elucidated. It represents the first example of acylation with 3,4-dihydroxyphenyl acetic acid in flavonoid chemistry. The in-vitro cytotoxicity studies against a human keratinocytes cell line revealed the non-toxicity of PPEE and PPEE-SLNs. Moreover, PPEE, PPEE-SLNs, and KDPAG showed good anti-elastase activity, comparable to that of N-(Methoxysuccinyl)-Ala-Ala-Pro-Val-chloromethyl ketone. Besides, PPEE-SLNs and KDPAG showed significantly (p < 0.001) higher anti-collagenase and anti-tyrosinase activities in comparison to EDTA and kojic acid, respectively. Different PPEE-SLNs cream formulae (2% and 5%) were evaluated for possible anti-wrinkle activity against UV-induced photoaging in a mouse model using a wrinkle scoring method and were shown to offer a highly significant protective effect against UV, as evidenced by tissue biomarkers (SOD) and histopathological studies. Thus, the current study demonstrates that Prunus persica leaf by-products provide an interesting, valuable resource for natural cosmetic ingredients. This provides related data for further studying the potential safe use of PPEE-SLNs in topical anti-aging cosmetic formulations with enhanced skin permeation properties