Browsing by Author "Mokhtar, Fatma A"

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    Correction: Network pharmacology and molecular docking study for biological pathway detection of cytotoxicity of the yellow jasmine flowers
    (BioMed Central Ltd., 2023-06) El‑Hawary, Seham S; Albalawi, Marzough A; Montasser, Ayat O; Ahmed, Shaimaa R; Qasim, Sumera; Shati, Ali A; Alfaif, Mohammad Y; Elbehairi, Serag Eldin I; Hassan, Omnia F; Sadakah, Abdelfattah A; Mokhtar, Fatma A
    Following publication of the original article [1], the authors would like to remove the affiliation ‘Cell Culture Lab, Egyptian Organization for Biological Products and Vaccines (VACSERA Holding Company), Giza, Egypt’ to author Serag Eldin I. Elbehairi. The author group has been updated above and the original article has been corrected. © 2023, The Author(s).
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    Green Biosynthesis of Silver Nanoparticles Using Annona glabra and Annona squamosa Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling
    (Multidisciplinary Digital Publishing Institute (MDPI), 2022-11) Mokhtar, Fatma A; Selim, Nabil M; Elhawary, Seham S; Abd El Hadi, Soha R; Hetta, Mona H; Albalawi, Marzough A; Shati, Ali A; Alfaifi, Mohammad Y; Elbehairi, Serag Eldin I; Fahmy, Lamiaa I; Ibrahim, Rana M
    Annona glabra L. (AngTE) and Annona squamosa L. (AnsTE) fruits have been widely used in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling was established using UPLC-QTOF-MS/MS. All species were tested for anticancer activity against human cervical cancer cells (HeLa), prostate adenocarcinoma metastatic (PC3), and ovary adenocarcinoma (SKOV3) using sulphorhodamine B assay. Apoptosis was determined using Annexin flow cytometry along with cell cycle analysis and supported by a molecular docking. The antibacterial and synergistic effect when combined with gentamicin were evaluated. A total of 114 compounds were tentatively identified, mainly acetogenins and ent-kaurane diterpenes. AnsTE and Ans-AgNPs had the most potent cytotoxicity on HeLa and SKOV3 cells, inducing a significant apoptotic effect against all tumor cells. The AnsTE and Ans-AgNPs significantly arrested PC3, SKOV3, and HeLa cells in the S phase. The nanoparticles demonstrated greater antibacterial and antifungal activities, as well as a synergistic effect with gentamicin against P. aeruginosa and E. coli. Finally, a molecular docking was attempted to investigate the binding mode of the identified compounds in Bcl-2 proteins’ receptor, implying that the fruits and their nanoparticles are excellent candidates for treating skin infections in patients with ovarian or prostatic cancer.
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    The medicinal activity of lyophilized aqueous seed extract of Lepidium sativum L. in an androgenic alopecia model
    (Nature Publishing Group, 2023-05) Albalawi, Marzough Aziz; Hafez, Ahmed M; Elhawary, Seham S; Sedky, Nada K; Hassan, Omnia F; Bakeer, Rofanda M; Abd El Hadi, Soha; El‑Desoky, Ahmed H; Mahgoub, Sebaey; Mokhtar, Fatma A
    This study evaluated the topical efect of Lepidium sativum lyophilized seed extract (LSLE) towards Sustanon-induced alopecia in male adult Wistar albino rats in vivo, compared to minoxidil topical reference standard drug (MRD). LC–MS/MS together with molecular networking was used to profle the metabolites of LSLE. LSLE treated group revealed signifcant changes in alopecia related biomarkers, perturbation of androgenic markers; decline in testosterone level and elevation in 5α-reductase (5-AR); decline in the cholesterol level. On the other hand, LSLE treated group showed improvement in vascular markers; CTGF, FGF and VEGF. Groups treated topically with minoxidil and LSLE showed signifcant improvement in hair length. LC–MS/MS profle of LSLE tentatively identifed 17 constituents: mainly glucosinolates, favonoid glycosides, alkaloids and phenolic acids. The results point to the potential role of LSLE in the treatment of alopecia through decreasing 5(alpha)- dihydrotestosterone levels. Molecular docking was attempted to evaluate the probable binding mode of identifed compounds to androgen receptor (PDB code: 4K7A).