Browsing by Author "Mohamed K."

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    Field-amplified sample stacking cyclodextrin modified capillary electrophoresis for quantitative determination of diastereomeric saponins
    (Springer Netherlands, 2014) Emara S.; Masujima T.; Zarad W.; Mohamed K.; Kamal M.; Fouad M.; EL-Bagary R.; Faculty of Pharmacy; Pharmaceutical Chemistry Department; Misr International University; Km 28 Ismailia Road; Cairo; Egypt; P.I. Laboratory Single Cell MS; RIKEN Quantitative Biology Center; 6-2-3; Furuedai; Suita; Osaka; 565-0874; Japan; Faculty of Pharmacy; Pharmacognosy Department; Assiut University; Assiut; 71526; Egypt; Faculty of Pharmacy; Pharmaceutical Analytical Chemistry Department; Modern Sciences and Arts University; 26 July Mehwar Road Intersection with Wahat Road; 6 October City; Egypt; Faculty of Pharmacy; Pharmaceutical Chemistry Department; Cairo University; Kasr El Aini St.; Cairo; 11562; Egypt
    Successful simultaneous diastereomeric separation and sensitive determination of two pairs of triterpenoidal saponins have been achieved by capillary electrophoresis (CE) using ?-cyclodextrin (?-CD) as a stereoselective agent to cooperate with borate complexation. A usual technique for isolation and group separation of saponins was developed as an appropriate purification step prior to the determination of individual saponins by CE. Soyasaponin I (S1), azukisaponin V (S2), bersimoside I (S3) and bersimoside II (S4) could be well separated within 14 min in a fused-silica capillary (60 cm long to the detector with an additional 10 cm to the cathode; 75 ?m i.d.). The background electrolyte was borate buffer (80 mM, pH 10), containing 24 mM ?-CD. The separation voltage was 14 kV with a detection wavelength of 195 nm. The sample was electrokinetically injected using a voltage of 16 kV for 12 s. Methanol (70%) was used as the diluent for field-amplified sample stacking after hydrodynamic injection of short water plug (5 cm, 4 s). The method was partially validated for linearity, repeatability, reproducibility, limits of detection and limits of quantification. The correlation coefficients of the calibration curves were all >0.998, and the recoveries were from 98.23 to 96.21%. The Author [2013]. Published by Oxford University Press. All rights reserved.