Browsing by Author "Mohamed, Mona"

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    COMPARATIVE EVALUATION OF THE ANTIBACTERIAL ACTIVITY OF GLASS IONOMER RESTORATION INCORPORATED WITH DIFFERENT METAL OXIDES NANOPARTICLES ON ORAL STREPTOCOCCUS MUTANS
    (EDJ, 2019-10) Ezzeldin, Naglaa; Elshishtawy, Hisham; El-Borady, Ola; Mohamed, Mona
    Objective: The objective of this study is to compare and evaluate the antibacterial activity of modified conventional Glass ionomer restoration (GI) with zinc oxide (ZnO-NPs) and cerium oxide Nanoparticles (CeO2-NPs) on the oral Streptococcus mutans (SM). Materials and Methods: one hundred and five disc-shaped specimens were divided into seven groups; GI, GI incorporated with three, five and seven percent ZnO-NPs and CeO2-NPs. Antibacterial activity was assessed by counting of colony forming unit (CFU) of SM after direct contact test and 96 hours biofilm evaluation. Fourier transform infrared spectroscopy (FTIR) was used to illustrate interaction between different concentrations of Nanoparticles incorporated with GI. Results: Direct contact test showed a significant reduction of CFU of GI incorporated with seven percent ZnO-NPs in relation to GI (P value 0.007). Biofilm evaluation showed significant reduction of CFU of GI incorporated with three, five and seven percent ZnO-NPs and five and seven percent CeO2-NPs (P value 0.000). FTIR showed Peaks at 1405 and 1635 cm–1 related to symmetric and asymmetric vibrations of COO−, those peaks were broader and red shifted in GI incorporated with ZnO-NPs and CeO-NPs. Conclusion: Incorporation of conventional GI with ZnO-NPs and CeO2-NPs enhance its antibacterial activity against oral SM.
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    Effect of Two Remineralizing Agents on Artificial Demineralized Enamel Irradiated with Er, Cr:YSGG Laser: In-Vitro Study
    (October university for modern sciences and Arts (MSA), 2022-11) Abdulla, Nada Mohamed; Mohamed, Mona; Abdel Mohsen, Mohamed Mahmoud
    Background: Dental caries is a widespread, noncommunicable disease affecting half of world’s population. Thus, prevention rather than mere treatment of dental caries has become a decisive goal in dentistry. Therefor the adequacy of laser (Erbium, chromium-doped yttrium, scandium, gallium and garnet) irradiation and remineralizing agent were tested on artificially demineralized enamel. Methodology: Twenty human molars were selected, scaled, polished and sectioned into two equal buccal and lingual sections (n=40). Surfaces were coated with acid resistance nail varnish except for enamel window of 4x4 mm. The specimens were embedded in demineralizing solution for 3 days. They were randomly divided into two groups (n=20) according to type of remineralizing agent, group (MI): MI paste plus and group (RE): Remin Pro. They were applied according to manufacturer’s instructions. The groups were then divided into two subgroups (n=10); groups (MIL) and (REL). In both groups specimens were treated first with Er, Cr: YSGG Laser then remineralizing agents were applied. The weight % of Calcium and Phosphate were measured using EDX. The measurements were done before any treatment (control group), after demineralization and after remineralization. Representative specimens were selected for scanning. Results: The results of the study showed that (MI, RE and MIL) weren’t able to restore the minerals compared to the control group. While REL group was the only group similar to the control group. In addition, the intergroup comparison showed that REL had the highest remineralization compared to other groups. Conclusions: The combined use of laser and remineralizing agents improved enamel remineralization
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    Inflammatory breast cancer: Mixed viral infections within carcinoma tissues and the expression of Ki-67 proliferation marker.
    (American Society of Clinical Oncology, 2017) taha Mohamed, Hossam; Hesham Abdel Fattah, Hadeer; El-Shinawi, Mohamed; Abdelaziz Ibrahim, Sherif; S. El-Halawany, Medhat; El Ghazaly, Hesham; Schneider, Robert; Mohamed, Mona
    Background: Inflammatory breast cancer (IBC) is the most lethal form of breast cancer. Our previous results showed that IBC carcinoma tissues possess mixed human cytomegalovirus genotypes than non-IBC carcinoma tissues. However, the role of viral infection in breast cancer is poorly understood. Methods: We enrolled 135 women diagnosed breast cancer (91 Non-IBC and 44 IBC). The incidence of different viral DNA (Herpes viruses and HPV) was performed using nested and multiplex PCR and DNA sequencing. The expression of Ki-67 proliferation index was assessed by immunohistochemistry. Results: DNA of HCMV and HPV-16 were the most detected in breast tissues of both IBC and non-IBC patients. However, as a single infection the incidence of HCMV-DNA and HHV-8 DNA were significantly higher in carcinoma tissues of IBC in comparison with non-IBC (p = 0.035, p= 0.039, respectively). Moreover, the prevalence of mixed infection of different viral DNA was higher in IBC than non-IBC carcinoma tissues (P= 0.003). HCMV and HPV-16 were the dominant mixed infection in both non-IBC and IBC tissues. Interestingly, although no significant difference in expression of Ki67 has been detected in tissues of IBC and non-IBC, we found that Ki-67 was significantly higher in mixed than single viral infected tissues of both non-IBC and IBC (p = 0.04 and p = 0.03 respectively). Conclusions: The incidence of mixed viral DNA detected in carcinoma tissues of IBC is higher than non-IBC. Moreover, mixed viral DNA is positively correlated with upregulation of Ki67 expression in breast carcinoma tissues.