Browsing by Author "Mohamed, Ayman Saber"

Now showing 1 - 5 of 5

Biointerfrence between Zinc Oxide/Alginate Nanocomposites and Freshwater Bivalve
(AMG Transcend Association, 2022-07) Omar, Tahani Younis; Elshenawy, Habiba Ihab Ahmed; Abdelfattah, Marwa Ahmed; Al Shawoush, Abdeljalil Mohamed; Mohamed, Ayman Saber; Saad, Dalia Y
Commercial usage of zinc oxide nanoparticles threatens the aquatic ecosystem. The green synthesis of ZnO nanoparticles is an environmentally benign method of nanoparticle manufacture. Aim: In the current study, freshwater bivalves were employed as a crucial indicator for the green synthesis of alginate/ZnO nanocomposite. Transmission electron microscopy, ultraviolet spectroscopy, and X-Ray diffraction were used to analyze the produced Alginate/ZnO nanocomposite. The bivalve was subjected to various dosages of ZnO and alginate/ZnO nanoparticles (12, 25, and 50 mg/L). The alginate/ZnO nanocomposite size was between (10-15 nm), whereas ZnO was between (5-10 nm). Malondialdehyde and nitric oxide levels increased at all Alginate/ZnO nanocomposite doses, whereas catalase and glutathione levels decreased in all organs. After exposure to alginate/ZnO nanocomposite nanoparticles, the gills and mantle histopathological examinations revealed changes. Incorporating alginate into the production of ZnONPs causes combinatorial harmful effects in Coelatora aegyptica, including oxidative stress and histopathological alterations. Coelatora aegyptiaca was found to be a sensitive bio- indicator for nanoparticle-induced water pollution.
The Concurrent Therapeutic Potential of Adipose-derived Mesenchymal Stem Cells on Gentamycin-induced Hepatorenal Toxicity in Rats
(Bentham Science Publishers, 2022-11) Rawash, Mohamed A; Mohamed, Ayman Saber; El-Zayat, Emad M
Background: Adipose mesenchymal stem cells (AMSCs) are a type of stem cell employed to repair damaged organs. This study aimed to see how effective AMSCs are at treating gentamycin- induced hepatorenal damage in rats. Methods: 18 male Wister rats were assigned into three groups; control, Gentamycin (GM), and GM+AMSCs. GM induced hepatorenal toxicity through daily injection (100 mg/kg, i.p.) for eight days. On day 9, AMSC (106 cells/ml/rat) was injected intravenously. Results: Creatinine, urea, uric acid, AST, ALP, ALT, TNF-, and MDA levels decreased, whereas IL-10, GSH, and CAT levels increased, indicating the therapeutic potency of intravenous injection AMSCs. Conclusion: The current study demonstrated the simultaneous therapeutic efficacy of adipose mesenchymal stem cells on the liver and kidney in the treatment of Gentamycin-induced hepatotoxicity. These data show that AMSCs could be a feasible therapy option for liver and kidney disease.
The concurrent therapeutic potential of adipose-derived mesenchymal stem cells on Gentamycin-induced hepatorenal toxicity in rats
(Bentham Science Publisher, 2021-10) Rawash, Mohamed A; Mohamed, Ayman Saber; El-Zayat, Emad M
Background: Adipose mesenchymal stem cells (AMSCs) are a type of stem cell employed to repair damaged organs. This study aimed to see how effective AMSCs are at treating gentamycin-induced hepatorenal damage in rats. Methods: 18 male Wister rats were assigned into three groups; control, Gentamycin (GM), and GM+AMSCs. GM induced hepatorenal toxicity through daily injection (100 mg/kg, i.p.) for eight days. On day 9, AMSC (106 cells/ml/rat) was injected intravenously. Results: Creatinine, urea, uric acid, AST, ALP, ALT, TNF-, and MDA levels decreased, whereas IL-10, GSH, and CAT levels increased, indicating the therapeutic potency of intravenous injection AMSCs. Conclusion: The current study demonstrated the simultaneous therapeutic efficacy of adipose mesenchymal stem cells on the liver and kidney in the treatment of Gentamycin-induced hepatotoxicity. These data show that AMSCs could be a feasible therapy option for liver and kidney disease
Therapeutic effect of Nigella sativa extract on folic acid-induced acute hepatorenal injury: influences and underlying mechanisms
(Research Trends, 2023-06) Al Shawoush, Abdeljalil Mohamed; Said, Reem Sherif; Hassan, Fatma E; Ali, Sara Bayoumi; Mohamed, Ayman Saber; Elbatran, May Mohamed
Nigella sativa is utilized to treat many ailments and to maintain and improve global health as it has numerous pharmacological activities such as anti-inflammatory, antidiabetic, antimetastatic, anxiolytic, hepatoprotective and immunomodulatory. We tried to assess the disorders in the liver, the main organ for folate metabolism, and the kidney in response to high-dose folic acid (HDFA) supplementation, and we tested if the herbal Nigella sativa extract (NSE) could alter these disturbances and the likely underlying mechanisms. Eighteen adult male rats were separated into three groups: Control, HDFA and HDFA+NSE. Both kidney and liver dysfunctions induced by HDFA administration significantly improved by NSE which was supported by histological examination. Thus, NSE being an ample source of antioxidants has a hepatorenal protecting effect.
Therapeutic potency of gallium verum extract on ethanol-induced gastric ulcer in rats
(AMG Transcend Association, 15/10/2022) Lotfy, Bassant Mohamed Mohamed; Mousa, Mohamed R; El-Shehry, Mona Samir Fawzy El-Sayed; Ahmed, Salma Hussein Abdel-Fattah; Ali, Sara Bayoumi; Al Shawoush, Abdeljalil Mohamed; Mohamed, Ayman Saber
Gallium verum, lady's bedstraw is an herbaceous annual plant belonging to the genus Rubiaceae; it possessed antioxidant, cytotoxic for cancer cells, antimicrobial, protective and endocrine effects. This work aimed to investigate the curative effects of G. verum extract on gastric ulcers following absolute ethanol administration in the healthy rat. Eighteen rats were randomly divided into three main groups; rats were fastened for 24 hours before ethanol administration. All groups except control administered ethanol (5 ml/kg body weight; orally). The rats were administrated distilled water (Ulcer group) or G. verum extract (100mg/kg) one hour later. G. verum extract caused a significant decrease in ulcer index, gastric juice volume, malondialdehyde, and nitric oxide, while gastric juice pH, glutathione, glutathione-S-transferase, and catalase increased significantly. The histological lesion score showed a significant enhancement in group G. verum compared to the ulcer group that scored the highest pathological destruction score. Immunohistochemical markers of NF-κB p65 and TNF-α showed a significant decrease in G. verum group. G. verum extract is a promising treatment modality against gastric injury through its powerful antioxidant, acid neutralizing, healing promotion, and ant- inflammation effects.