Browsing by Author "Meyer A."
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Item Effect of oxylipins, terpenoid precursors and wounding on soft corals' secondary metabolism as analyzed via UPLC/MS and chemometrics(MDPI AG, 2017) Farag M.A.; Westphal H.; Eissa T.F.; Wessjohann L.A.; Meyer A.; Department of Pharmacognosy; College of Pharmacy; Cairo University; Kasr El Aini St.; Cairo; 11562; Egypt; Department of Chemistry; School of Sciences and Engineering; American University in Cairo; New Cairo; 11835; Egypt; Leibniz Centre for Tropical Marine Research; Fahrenheit Str.6; Bremen; D-28359; Germany; Department of Geosciences; Bremen University; Fahrenheit Str.6; Bremen; D-28359; Germany; Department of Pharmacognosy; College of Pharmacy; Modern Science and Arts University; Cairo; 12566; Egypt; Department of Bioorganic Chemistry; Leibniz Institute of Plant Biochemistry; Weinberg 3; Halle; D-06120; GermanyThe effect of three oxylipin analogues, a terpenoid intermediate and wounding on the secondary metabolism of the soft corals Sarcophyton glaucum and Lobophyton pauciflorum was assessed. Examined oxylipins included prostaglandin (PG-E1), methyl jasmonate (MeJA), and arachidonic acid (AA) in addition to the diterpene precursor geranylgeranylpyrophosphate (GGP). Post-elicitation, metabolites were extracted from coral heads and analyzed via UPLC-MS followed by multivariate data analyses. Both supervised and unsupervised data analyses were used for sample classification. Multivariate data analysis revealed clear segregation of PG-E1 and MeJA elicited S. glaucum at 24 and 48 h post elicitation from other elicitor samples and unelicited control group. PG-E1 was found more effective in upregulating S. glaucum terpene/sterol levels compared to MeJA. Metabolites showing upregulation in S. glaucum include campestene-triol and a cembranoid, detected at ca. 30- and 2-fold higher levels compared to unelicited corals. Such an elicitation effect was less notable in the other coral species L. pauciflorum, suggesting a differential oxylipin response in soft corals. Compared to MeJA and PG, no elicitation effect was observed for GGP, AA or wounding on the metabolism of either coral species. � 2017 by the authors. Licensee MDPI, Basel, Switzerland.