Browsing by Author "Mamdouh S."

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Evaluation of Mir-224, Mir-215 and Mir-143 as Serum biomarkers for HCV associated Hepatocellular carcinoma
(Asian Pacific Organization for Cancer Prevention, 2017) Mamdouh S.; Khorshed F.; Aboushousha T.; Hamdy H.; Diab A.; Seleem M.; Saber M.; Department of Biochemistry and Molecular Biology; Theodor Bilharz Research Institute; October University for Modern Sciences and Arts; Giza; Egypt; Department of Pathology; October University for Modern Sciences and Arts; Giza; Egypt; Department of Surgery; Theodor Bilharz Research Institute; October University for Modern Sciences and Arts; Giza; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; Giza; Egypt; Department of Surgery; National Hepatology and Tropical Medicine Research Institute; Cairo; Egypt
HCV induced hepatitis and hepatocellular carcinoma as its sequel are major health problems world-wide and especially in Egypt. For diagnosis and during treatment of liver diseases, liver functions are monitored through determination of serum levels of liver enzymes and a-fetoprotein although the obtained information is generally not sufficient for either early detection of hepatic insult or effective follow up of therapeutic effects. More sensitive biomarkers may help to achieve these goals. MiRNAs are small non-coding RNAs that have an important role in gene expression and regulation. Many, such as miR-224, miR-215, miR-143 are correlated with tumor appearance and with the degree of fibrosis in lung, breast and colon cancer. This study was performed to estimate the level of these miRNAs in serum of patients with HCV-associated hepatitis and HCC in relation to grade of hepatitis, stage of fibrosis and differentiation of tumor tissue. In addition, correlations between serological and tissue levels were assessed. A total of 80 patients were examined, out of which 50 were included in the study. Blood samples and tissue specimens from malignant tumor and corresponding non-tumor tissue of HCV hepatitis patients were collected. Blood samples from 20 healthy volunteers were also obtained as controls. It was found that miRNAs profiles differed in HCC patients compared to controls and HCV-associated hepatitis cases. Distinction of tumor grade and fibrosis stage of patients as well as between different grades of tumor differentiation proved possible, making miRNAs promising biomarkers for diagnosis and assessment of treatment response of HCC patients.
Immunohistochemical and biochemical expression patterns of TTF-1, RAGE, GLUT-1 and SOX2 in HCV-associated hepatocellular carcinomas
(Asian Pacific Organization for Cancer Prevention, 2018) Aboushousha T.; Mamdouh S.; Hamdy H.; Helal N.; Khorshed F.; Safwat G.; Seleem M.; Pathology Departmenty; ; Giza; Egypt; Biochemistry Departmenty; ; Giza; Egypt; Surgical Department; Theodor Bilharz Research Institutey; ; Giza; Egypt; Faculty of Biotechnology; ; Giza; Egypt; National Hepatology and Tropical Medicine Research Institute; Cairo; Egypt
Objective: To investigate the expression of TTF-1, RAGE, GLUT1 and SOX2 in HCV-associated HCCs and in surrounding non-tumorous liver tissue. Material and Methods: Tissue material from partial hepatectomy cases for HCC along with corresponding serum samples and 30 control serum samples from healthy volunteers were studied. Biopsies were classified into: non-tumor hepatic tissue (36 sections); HCC (33 sections) and liver cell dysplasia (LCD) (15 sections). All cases were positive for HCV. Immunohistochemistry (IHC), gene extraction and quantitative real-time reverse-transcription assays (qRT-PCR) were applied. Results: By IHC, LCD and HCC showed significantly high percentages of positive cases with all markers. SOX2 showed significant increase with higher HCC grades, while RAGE demonstrated an inverse relation and GLUT-1 and TTF-1 lacked any correlation. In nontumorous-HCV tissue, we found significantly high TTF-1, low RAGE and negative SOX2 expression. RAGE, GLUT-1 and SOX2 show non-significant elevation positivity in high grade HCV compared to low grade lesions. TTF-1, RAGE and SOX2 exhibited low expression in cirrhosis compared to fibrosis. Biochemical studies on serum and tissue extracts revealed significant down-regulation of RAGE, GLUT-1 and SOX2 genes, as well as significant up-regulation of the TTF-1 gene in HCC cases compared to controls. All studied genes show significant correlation with HCC grade. In non-tumor tissue, only TTF-1 gene expression had a significant correlation with the fibrosis score. Conclusion: Higher expression of TTF-1, RAGE, GLUT-1 and SOX2 in HCC and dysplasia compared to non-tumor tissues indicates up-regulation of these markers as early events during the development of HCV-associated HCC.