Browsing by Author "Makky, Amna M. A"

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Atorvastatin loaded lecithin‑coated zein nanoparticles based thermogel for the intra‑articular management of osteoarthritis: in‑silico, in‑vitro, and in‑vivo studies
(Springer Netherlands, 2024-02) Elgendy, Heba Amin ; Makky, Amna M. A; Elakkad, Yara E; Awad, Heba H; El Hassab, Mahmoud A; Younes, Nihal Farid
Purpose Up-to-date literature ofers limited data about utilizing atorvastatin calcium (ATV) as a promising chondroprotective agent in osteoarthritis (OA). So, this study aims to develop a depot intra-articular (IA) delivery system for ATV to enhance its deposition in the articular joint. Methods A 33 D-optimal design was implemented to prepare ATV-loaded lecithin-coated zein nanoparticles. The optimized formulation (Opt-LCZN) was selected and imaged using a transmission electron microscope according to the desirability value. Various in-vitro and in-silico studies were conducted to evaluate the features of Opt-LCZN. Additionally, it was loaded into an injectable thermogel (Opt-LCZN-thermogel) and evaluated in-vivo in OA-induced Sprague Dawley rats. Results The Opt-LCZN showed entrapment efciency of 70.00±2.96%, particle size of 191.95±17.42 nm, zeta potential of − 20.12±0.79 mV, and polydispersity index of 0.25±0.01. The docking studies revealed favorable binding of zein and ATV, confrmed by molecular dynamics simulation. The morphological examination displayed a bilayer spherical structure formed of a zein core enclosed by a lecithin coat. Furthermore, the formulated Opt-LCZN-thermogel achieved a remarkable sustained release profle, with nearly 50% of the drug being released over 144 h. Opt-LCZN-thermogel showed a signifcant reduction in infammation in OA-induced rats, confrmed by knee joint swelling and knee bend test results, in addition to the pro-infammatory and anti-infammatory mediators’ levels. The protective efect of ATV can be markedly observed through histopathological examination. Conclusion Based on these outcomes, the formulated IA delivery system of ATV can be presented as an excellent candidate for ameliorating OA.
A Full Factorial Design to Optimize Aminexil Nano Lipid Formulation to Improve Skin Permeation and Efcacy Against Alopecia
(Springer International Publishing AG, 2023-01) Makky, Amna M. A; El‑leithy, Eman S; Hussein, Doaa Galaa ; Khattab, Abeer
Aminexil (AMX) is considered to be one of the most widely used hair growth promoters. Nanostructured lipid carriers (NLC) are employed to increase the permeation of both lipophilic and hydrophilic drugs. Aminexil nanostructured lipid carrier (NLC) designed by pre-emulsion/ultrasonication method was utilized for alopecia treatment. For selecting optimum excipients, a solubility study was executed in liquid lipids, solid lipids, surfactants, and co-surfactants. A 23 full factorial design was utilized for NLC optimization. Characterization of the developed formulas was performed. The penetration of the optimized formula across cuticle tissues was studied using confocal laser scanning microscopy (CLSM). AMX showed high solubility in glyceryl monostearate (GMS) and stearic acid, 28.87±2.17 and 58.06±2.227 mg/g, respectively. The results of physicochemical characterization showed that formula A7 was the optimized one. It is composed of GMS (solid lipid), oleic acid:garlic oil (1:1 v/v) (liquid lipid), and a surfactant/co-surfactant mixture (Cremophor EL/Transcutol HP). The particle size (PS) was 238.0±2.13 nm, entrapment efciency (EE) 100.535±6.73%, and zeta potential (ZP)−29.3±0.93 mv. Ex vivo permeation study demonstrates the potential of AMX-NLC (formula A7) as a delivery system for AMX. The CLSM highly proved AMX-loaded NLC penetration through the skin. The histological study clearly demonstrated that AMX-loaded NLC promoted hair growth more efectively than the market product in chemotherapy-induced alopecia rats. The acquired fndings revealed that targeting of AMX-loaded NLC into hair follicles was improved.