Browsing by Author "Mahmoud O.A."

Now showing 1 - 2 of 2

Comparative study of reversed-phase high-performance liquid chromatography versus thin-layer chromatography-densitometry for determination of Citicoline sodium in presence of its alkaline degradation products
(Akademiai Kiado Rt., 2015) Mahmoud O.A.; Hegazy M.A.; Salem H.; Moustafa A.A.; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); October City; 11787; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini Street; Cairo; 11562; Egypt
Simple, sensitive, selective, and precise stability-indicating thin-layer chromatography (TLC)-densitometric and reversed-phase high-performance liquid chromatography (RP-HPLC) methods were developed and validated for the determination of citicoline sodium (CT) in the presence of its alkaline degradation products (CT Deg.) and in pharmaceutical oral solution. TLC-densitometry employs aluminum TLC plates precoated with silica gel 60 F254 as the stationary phase and ammonia-ethyl acetate-triethylamine (6:3.5:0.5, v/v) as the mobile phase to give compact spots for citicoline sodium (RF = 0.35) and its degradation product (RF = 0.1); the chromatogram was scanned at 272 nm. RP-HPLC utilizes a C18 column and a mobile phase consisting of methanol-water-acetic acid (60:40:0.1, v/v); the pH level was adjusted to 4 using 0.1% orthophosphoric acid, at a flow rate of 1 mL min-1 for the separation of citicoline sodium (tR = 1.801) and its degradation product (tR = 3.422). Quantitation was achieved by ultraviolet (UV) detection at 272 nm. Citicoline sodium was exposed to alkaline hydrolysis, and a comparative study was carried out to show the advantages of the proposed chromatographic methods in determination of citicoline sodium in the presence of its alkaline degradation products. The chromatographic methods were developed and validated as per the International Conference on Harmonization guidelines. As the methods could effectively separate the drug from their degradation products, these techniques can be employed as stability-indicating methods that have been successively applied to pharmaceutical formulations without interference from the excipients. � Akad�miai Kiad�, Budapest.
Simultaneous determination of carbinoxamine, pholcodine, and ephedrine in antitussive preparation by high-performance liquid chromatography and thin-layer chromatography-densitometry
(Akademiai Kiado Rt., 2015) Moustafa A.A.; Salem H.; Hegazy M.; Mahmoud O.A.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini Street; Cairo; 11562; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Deraya University; Cairo; 14511; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October City; 11787; Egypt
Simple, accurate, precise, sensitive, and validated high-performance liquid chromatography (HPLC) and thin-layer chromatography (TLC)-densitometric methods were developed for the simultaneous determination of carbinoxamine (CAR), pholcodine (PHL), and ephedrine (EPH) in antitussive syrup. In method A, reversed-phase (RP)-HPLC analysis was performed on an Inertsil CN-3 column (250 mm � 4.6 mm, 5 ?m), using a mobile phase consisting of acetonitrile-distilled water (pH 3.5) using orthophosphoric acid in the ratio 70:30 (v/v) and flow rate of 1.5 mL min-1. Quantitation was achieved with ultraviolet (UV) detection at 220 nm. In method B, TLC analysis was carried out on an aluminum-backed sheet of silica gel 60 F254 layer using chloroform- propanol-ammonia (6:4:0.1, v/v) as the mobile. Quantification was carried out with UV detection at 245 nm. The validation of the proposed methods was applied according to the International Conference on Harmonization (ICH) guidelines. The suggested methods were successfully applied for the determination of the cited drugs in bulk powder and commercial dosage form. � Akad�miai Kiad�, Budapest.