Browsing by Author "M Aref, Ahmed"

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Cinnamic acid Attenuates Cisplatin-Induced Hepatotoxicity and Nephrotoxicity
(Journal of Basic and Environmental Sciences, 2016) A Tohamy, Amany; M Aref, Ahmed; E Abdel Moneim, Ahmed; H Sayed, Romissaa
We investigated the effects of cinnamic acid (CA, 20 mg/kg body weight) oncisplatin (CP)-induced hepto and nephrotoxicity in mice. CP (5 mg/kg bwt)wasinjected intraperitoneally and CA was given by gastric gavage for 5 days pre-andpost-CP injection. After 5 days of CP injection, CP-induced injuries of the hepaticand renal tissues which were evidenced (i) histopathological damage of the hepaticandrenal tissues, (ii) as increases in liver and kidney function parameters, (iii) asincreases in lipid peroxidation and nitric oxide, and (iv) as decrease in glutathionecontent. In contrast, the oral administration of CA concurrently to CP intoxicatedmice brought back lipid peroxidation, nitric oxide, glutathione levels to nearnormalcy. Moreover, the histological observations evidenced that CA effectivelyrescues the liver and kidney from CP mediated oxidative damage. Therefore,cinnamic acid can be considered a potential candidate for protection of hepato-andnephrotoxicity induced by cisplatin.
Expression of p53 Protein in Hepatocellular Carcinoma: Relationships to Viral Infection and Prognostic Factors
(Received: 17 January, 2010. Accepted: 11 May, 2010.Original Research PaperDynamic Biochemistry, Process Biotechnology and Molecular Biology ©2010 Global Science Book, 2010) E Helal, Thanaa; A El-Ganzuri, Monir; M Aref, Ahmed; T Badr, Mohamed
Hepatocellular carcinoma (HCC) is very common in Egypt due to the high incidence of hepatitis C virus (HCV) infection. The aim of the current study was to assess the relationships between p53 and the viral infection as well as the prognostic factors in patients with HCC. p53 protein was examined by means of immunohistochemistry in tissue sections from 34 HCCs and 16 cirrhotic patients. p53 was expressed in 12 out of 34 HCCs (35%). All the 16 cirrhotic cases were p53-negative. No significant relationship was detected between p53 expression and the prognostic factors(P > 0.05). However, the level of p53 expression was significantly higher in patients coinfected with HCV and hepatitis B virus (HBV) than in those having single infection.
hca519/TPX2: a potential T-cell tumor-associated antigen for human hepatocellular carcinoma
(Dove Press, 2014) M Aref, Ahmed; T Hoa, Neil; Ge, Lisheng; Agrawal, Anshu; Dacosta-Iyer, Maria; Lambrecht, Nils; Ouyang, Yi; N Cornforth, Andrew; R Jadus, Martin
Immunotherapy for human hepatocellular cancer (HCC) is slowly making progress towards treating these fatal cancers. The identification of new antigens can improve this approach. We describe a possible new antigen, hepatocellular carcinoma‐associated antigen‐519/targeting protein for Xklp‐2 (HCA519/TPX2), for HCC that might be beneficial for T‐cell specific HCC immunotherapy.
The Potential Role ofAzadirachta indicaTreatment onCisplatin-Induced Hepatotoxicity and Oxidative Stressin Female Rats
(Hindawi, 2013) M Aref, Ahmed; A Dkhil, Mohamed; Al-Quraishy, Saleh; S Othman, Mohamed; M. El-Deib, Kamal; E Abdel Moneim, Ahmed
Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver
Rutin and Selenium Co-administration Reverse 3-Nitropropionic Acid-Induced Neurochemical and Molecular Impairments in a Mouse Model of Huntington’s Diseas
(SPRINGER, 2020) S Abdelfattah, Mohamed; EA Badr, Sherif; A Lotfy, Sally; H Attia, Gouda; M Aref, Ahmed; E Abdel Moneim, Ahmed; B Kassab, Rami
Systemic administration of 3-nitropropionic acid (3-NPA) is commonly used to induce Huntington’s disease (HD)-like symptoms in experimental animals. Here, the potential neuroprotective efficiency of rutin and selenium (RSe) co-administration on 3-NPA-induced HD-like symptoms model in mice was investigated. 3-NPA injection evoked severe alterations in redox status, as indicated via increased striatal malondialdehyde and nitric oxide levels, accompanied by a decrease in levels of antioxidant molecules including glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase. Moreover, 3-NPA potentiated inflammatory status by enhancing the production of interleukin-1β, tumor necrosis factor-α, and myeloperoxidase activity. Pro-apoptotic cascade was also recorded in the striatum as evidenced through upregulation of cleaved caspase-3 and Bax, and downregulation of Bcl-2. 3-NPA activated astrocytes as indicated by the upregulated glial fibrillary acidic protein and inhibited brain-derived neurotrophic factor. Furthermore, perturbations in cholinergic and monoaminergic systems were observed. RSe provided neuroprotective effects by preventing body weight loss, oxidative stress, neuroinflammation, and the apoptotic cascade. RSe inhibited the activation of astrocytes, increased brain-derived neurotrophic factor, and improved cholinergic and monoaminergic transmission following 3-NPA intoxication. Taken together, RSe co-administration may prevent or delay the progression of HD and its associated impairments through its antioxidant, anti-inflammatory, anti-apoptotic, and neuromodulatory effects
Serum Levels of Interleukin-6 and Interleukin-10 as Biomarkers for Hepatocellular Carcinoma in Egyptian Patients
(Hindawi Publishing Corporation, 2015) M Aref, Ahmed; S Othman, Mohamed; A Mohamed, Amal; A Ibrahim, Wesam
Interleukin-10 (IL-10) and interleukin-6 (IL-6) have been reported to be related to hepatocellular carcinoma (HCC) prognosis. This study aimed to investigate the clinical usefulness of serum levels of IL-6 and IL-10 as biomarkers for HCC among high-risk patients.