Browsing by Author "Lotfy, H.M"

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    Analytical tools for greenness assessment of chromatographic approaches: Application to pharmaceutical combinations of Indapamide, Perindopril and Amlodipine
    (Elsevier Inc., 2020-12) Saleh, S.S; Lotfy, H.M; Tiris, G; Erk, N; Rostom, Y
    The green profile of the proposed method was assessed and compared with reported classical methods via four tools of greenness which are: Eco-Scale, National Environmental Methods Index (NEMI), Assessment of Green Profile (AGP) and Green Analytical Procedure Index (GAPI). A simple, precise and rapid (RP-HPLC) has been developed for the quantification of Indapamide (IND), perindopril (PER) arginine and amlodipine besylate (ADB) in binary and ternary mixtures. A greener alternate RP-HPLC method was presented for the determination of pharmaceutical combinations composed of the cited medications using an eco-friendly eluent and quick run time with the least waste yield. Chromatographic separation was achieved using Waters Spherisorb ODS-2 C18 column (150 × 4.6 mm, i.d., 5 μm) and eluent formed of acetonitrile: phosphate buffer (20 mM, pH = 3): methanol in the ratio of (65: 30: 5, by volume) at flow rate 1.0 mL/min with DAD-detection at 220 nm. This procedure was valid over linearity ranges of 0.5–20 μg/mL, 2.5–40 μg/mL and 2–40 μg/mL for IND, PER and AML, respectively. The presented chromatographic method was completely validated regarding ICH guidelines and were statistically compared with those of the reported methods applying t-test and F-test at 95% confidence. The proposed method was found to be greener in terms of usage of hazardous chemicals and solvents, energy consumption, and waste production. © 2020 Elsevier B.V
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    Trade-off efficacy and data processing strategy in the power of spectral resolution of co-formulated antihypertensive pharmaceuticals
    (Elsevier B.V., 2/15/2021) Rostom, Y; Lotfy, H.M; Öztürk, M; Tiris, G; Erk, N; Saleh, S.S
    Versatile, extraction-free univariate spectrophotometric methods have been modified to get effective spectral resolution of mixtures in accordance with their feature challenges. The proposed methods have been applied and validated for analyzing some antihypertensive medicines in their co-formulated medicinal products. Two mixtures have been used: the first one [Mix I (OLM/ADB)] is composed of Olmesartan medoxomil (OLM) and Amlodipine besylate (ADB) with partly-overlapped spectra while the second [Mix II (TEL/HCT)] is made up Telmisartan (TEL) and Hydrochlorothiazide (HCT) with total-overlapped spectra. Induced dual wavelength, absorbance correction and ratio subtraction coupled with constant multiplication methods were applied to Mix I (OLM/ADB), while dual wavelength, advanced absorbance subtraction and constant center coupled with spectrum subtraction methods were applied to Mix II (TEL/HCT). Calibration graphs were established with good correlation coefficients. The methods exhibit significant advantages as simplicity, sensitivity, minimal data manipulation besides optimum robustness. Selectivity was inspected using lab-mixtures with diverse ratios. Accuracy, precision and repeatability were found to be within the acceptable limits. The proposed methods are good enough to be used for co-assay of analytes in combined forms without any interfering from excipients. Moreover, all results were estimated in accordance with ICH criteria and successfully compared with those of the reported methods applying t-test and F-test at 95% confidence level. Generally, these methods can be used efficiently for routine quality control testing. © 2020 Elsevier B.V