Browsing by Author "Lamiaa I. Fahmy"

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Computational metal-flavonoids complexes presentation of greenly synthesized silver nanoparticles combined flavonoids from Lens culinaris L. as anticancer agents using BcL-2 and IspC proteins
(Taylor and Francis Ltd, 2024-10-01) Heba W. Alhamdi; Fatma Alzahraa Mokhtar; Fouad Lamghari Ridouane; Ali A. Shati; Serag Eldin I. Elbehairi; Lamiaa I. Fahmy; Mohammad Y. Alfaifi; Nada K. Sedky; Heba A. Fahmy
Lens culinaris L., has been widely recognized for its medical applications. LC-ESI-TOF-MS identified 22 secondary metabolites including phenolics, flavonoids, and anthocyanidin glycosides among its total extract (LCTE). The study aimed to apply LCTE as a biogenic material for reducing and capping the silver nanoparticles (LC-AgNPs). The ynthesized LC-AgNPs were characterized using different techniques. The UV absorption was observed at λmax 379 nm. LC-AgNPs were spherical, with 19.22 nm average size. The face cubic centre nature was demonstrated by HR-TEM and XRD. The LC-AgNPs were then evaluated for their anticancer and antimicrobial potentials. LC-AgNPs showed an extremely potent cytotoxic activity against MCF-7, HCT-116 and HepG2 cell lines (IC50= 0.37, 0.35 and 0.1 µg/mL, respectively). LC-AgNPs induced significant apoptotic effects in the three examined cancer cell lines. LC-AgNPs resulted in sequestration of cells in G1 phase of the cell cycle in both MCF-7 and HCT-116 cells, meanwhile it trapped cells at the G2 phase in HepG2 cells. Moreover, the antimicrobial activity of LC-AgNPs was highly confirmed against Klebsiella pneumoniae and Acinetobacter baumannii. Molecular docking study designated Kaempferol-3-O-robinoside-7-O-rhamnoside and Quercetin-3-D-xyloside as the topmost LCTE active constituents that caused inhibition of both Bcl-2 and IspC cancer targets in combination with the produced silver nanoparticles.
Evaluating the risk of listeriosis through genotypic profiling of potentially hazardous strains isolated from local food market
(Zagazig University, 2024-11-02) Lamiaa I. Fahmy; Heba M. Amin
Background: Listeriosis is a serious food-borne disease caused by Listeria monocytogenes pathogen that causes fatal systemic infections in the elderly and immunocompromised people. Listeria monocytogenes can be transferred from contaminated ready-to-eat or frozen foods. This study aimed to investigate the most prevalent serotypes of Listeria monocytogenes isolates in frozen food in Egypt, their phenotypic and genotypic antimicrobial resistance, biofilm formation, as well as virulence encoding genes. Methods: A total of 331 frozen food product samples were randomly chosen from various marketplaces and cultured for the presence of Listeria monocytogenes. The antibiogram of the isolated Listeria monocytogenes was assessed using the Kirby– Bauer disk diffusion method. Polymerase chain reaction (PCR) was performed to detect four antimicrobial resistance encoding genes and seven virulence genes. A multiplex PCR assay was performed. Results: Listeria monocytogenes was found in 14.2% (47/331) of the examined samples, and the most predominant serotypes belonged to molecular sero-groups 1/2a-3a. Listeria monocytogenes bacteria showed high phenotypic resistance rates to ampicillin (91%), cefotaxime (87%) and clindamycin (66%). Strong and moderate biofilm producers accounted for 21.4% and 11.9% of isolates, respectively. The most commonly detected genetic markers of resistance were strB and tetA genes (70%). The virulence genes: plcB, prs-prfA, iap and hlyA genes, were detected in 90%,87%, 87% and 30% of the isolates, respectively, while plc A, prfA and flA genes were detected in 83%, 97% and 90% of the isolates, respectively. Conclusion: Listeria monocytogenes food pathogen exists with considerable rates in frozen products, which may seriously threaten public health.