Browsing by Author "Kumosani, Taha Abullah"

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    Khalas date flavonoids inhibited cell viability, induced apoptosis and expression of the pro-autophagy LC3-B gene in human hepatocellular carcinoma cells (HepG2)
    (Taylor and Francis Ltd., 2022-11) AL-Ghamdi, Maryam Abdu; Alsulami, Rawyah Radi; Bakkar, Ashraf; Kumosani, Taha Abullah; Barrbour, Elie Kamil; Abulnaja, Khalid Omar; Huwait, Etimad; Moselhy, Said Salama
    Autophagy is a protective mechanism important in human dis- eases as cancer. We evaluated the impact of khalas date extract (KDE) (20-60 mg/mL) on cell viability, morphological changes, DNA fragmentation and gene expression of LC3B-II associated with autophagosome on HepG2 cell line. The GC/MS identification of KDE showed its high content of flavonoids including quercetin, myricetin, kaempferol and catechol. KDE reduced cell viability of HepG2 with IC50 (31.52 mg/mL). Cells treated with KDE showed two band of DNA fragments at (30 and 40 mg) indicating that KDE induced DNA damage and apoptosis in HepG2. The analysis RT-PCR data showed a 0.2-fold increase in the expression of LC3- B in the cells treated with KDE versus control. We concluded that, KDE flavonoids such as quercetin, myricetin kaempferol exhibited anticancer properties manifested by inhibition of HepG2 cell via- bility and induction of apoptosis and upregulation of the pro- autophagy LC3-B gene. ARTICLE HISTORY