Browsing by Author "Kassab R.B."

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Oleuropein suppresses oxidative, inflammatory, and apoptotic responses following glycerol-induced acute kidney injury in rats
(Elsevier Inc., 2019) Yin M.; Jiang N.; Guo L.; Ni Z.; Al-Brakati A.Y.; Othman M.S.; Abdel Moneim A.E.; Kassab R.B.; Department of Nephrology; China-Japan Union Hospital of Jilin University; Changchun; Jilin 130033; China; Department of Human Anatomy; College of Medicine; Taif University; Taif; Saudi Arabia; B.Sc. Department; Preparatory Year College; University of Hail; Hail; Saudi Arabia; Faculty of Biotechnology; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; 11795; Egypt
Aim: Here, we evaluated the possible protective effects of oleuropein, the major phenolic constituent in virgin olive oil against glycerol-induced acute kidney injury (AKI) in rats. Main methods: Twenty-eight Sprague Dawley rats were allocated equally into four groups as follows: control group, oleuropein group (50 mg/kg body weight), AKI group and the oleuropein + AKI group. AKI was induced by injecting 50% glycerol (10 ml/kg body weight) intramuscularly. Key findings: Glycerol injection increased the kidney relative weight as well as rhabdomyolysis (RM)- and AKI-related index levels, including the levels of creatine kinase, lactate dehydrogenase, creatinine, urea, and Kim-1 expression. Additionally, alteration in oxidative conditions in renal tissue was recorded, as confirmed by the elevated malondialdehyde and nitric oxide levels and the decreased glutathione content. Concomitantly, the protein and mRNA expression levels of antioxidant enzymes were suppressed. Moreover, Nfe2l2 and Hmox1 mRNA expression was also downregulated. Glycerol triggered inflammatory reactions in renal tissue, as evidenced by the increased pro-inflammatory cytokines and Ccl2 protein and mRNA expression, whereas myeloperoxidase activity was increased. Furthermore, glycerol injection enhanced apoptotic events in renal tissue by increasing the expression of the pro-apoptotic proteins and decreasing that of anti-apoptotic. However, oleuropein administration reversed the molecular, biochemical, and histological alterations resulting from glycerol injection. Significance: Our data suggest that oleuropein has potential as an alternative therapy to prevent or minimize RM incidence and subsequent development of AKI, possibly due to its potent anti-stress, anti-inflammatory, and anti-apoptotic effects. � 2019
The potential protective effect of physalis peruviana L. against carbon tetrachloride-induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression
(Elsevier Ltd., 2014) Al-Olayan E.M.; El-Khadragy M.F.; Aref A.M.; Othman M.S.; Kassab R.B.; Abdel Moneim A.E.; Zoology Department; Faculty of Science; King Saud University; Riyadh 11451; Saudi Arabia; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo 11795; Egypt; Biological Science Department; Faculty of Dentistry; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Biochemistry and Molecular Biology Department; Faculty of Biotechnology; Modern Sciences and Arts (MSA) University; Giza 12111; Egypt; Experimental Biology Department; Faculty of Science; Masaryk University; 62500 Brno; Czech Republic; Biochemistry and Molecular Biology Department; Asturias Institute of Biotechnology; University of Oviedo; 33006 Oviedo; Spain
The active constituent profile in Cape gooseberry (Physalis peruviana L.) juice was determined by GC-MS. Quercetin and kaempferol were active components in the juice. In this study we have evaluated its potential protective effect on hepatic injury and fibrosis induced by carbon tetrachloride (CCl4). Twenty-eight rats divided into 4 groups: Group I served as control group, and Group II received weekly i.p. injection of 2 mL CCl4/kg bwt for 12 weeks. Group III were supplemented with Physalis juice via the drinking water. The animals of Group IV received Physalis juice as Group III and also were intraperitoneally injected weekly with 2 mL CCl4/kg bwt for 12 weeks. Hepatoprotective effect was evaluated by improvement in liver enzymes serum levels, reduction in collagen areas, downregulation in expression of the fibrotic marker MMP-9, reduction in the peroxidative marker malonaldehyde and the inflammatory marker nitric oxide, and restoration of the activity of antioxidant enzymatic and nonenzymatic systems, namely, glutathione content, superoxide dismutase, catalase, glutathione-S-transferase, glutathione peroxidase, and glutathione reductase activities. The results show that the potential hepatoprotective effects of Physalis peruviana may be due to physalis acts by promotion of processes that restore hepatolobular architecture and through the inhibition of oxidative stress pathway. � 2014 Ebtisam M. Al-Olayan et al.
Rutin and Selenium Co-administration Reverse 3-Nitropropionic Acid-Induced Neurochemical and Molecular Impairments in a Mouse Model of Huntingtons Disease
(Springer, 2020) Abdelfattah M.S.; Badr S.E.A.; Lotfy S.A.; Attia G.H.; Aref A.M.; Abdel Moneim A.E.; Kassab R.B.; Natural Products Research Unit (NPRU); Chemistry Department; Faculty of Science; Helwan University; Cairo; 11795; Egypt; Regional Center for Food and Feed (RCFF); Agriculture Research Center; Giza; Egypt; Department of Pharmacognosy; College of Pharmacy; Najran University; Najran; Saudi Arabia; Faculty of Biotechnology; Modern Sciences and Arts University (MSA); Giza; Egypt; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo; 11795; Egypt
Systemic administration of 3-nitropropionic acid (3-NPA) is commonly used to induce Huntingtons disease (HD)-like symptoms in experimental animals. Here, the potential neuroprotective efficiency of rutin and selenium (RSe) co-administration on 3-NPA-induced HD-like symptoms model in mice was investigated. 3-NPA injection evoked severe alterations in redox status, as indicated via increased striatal malondialdehyde and nitric oxide levels, accompanied by a decrease in levels of antioxidant molecules including glutathione, glutathione peroxidase, glutathione reductase, superoxide dismutase, and catalase. Moreover, 3-NPA potentiated inflammatory status by enhancing the production of interleukin-1?, tumor necrosis factor-?, and myeloperoxidase activity. Pro-apoptotic cascade was also recorded in the striatum as evidenced through upregulation of cleaved caspase-3 and Bax, and downregulation of Bcl-2. 3-NPA activated astrocytes as indicated by the upregulated glial fibrillary acidic protein and inhibited brain-derived neurotrophic factor. Furthermore, perturbations in cholinergic and monoaminergic systems were observed. RSe provided neuroprotective effects by preventing body weight loss, oxidative stress, neuroinflammation, and the apoptotic cascade. RSe inhibited the activation of astrocytes, increased brain-derived neurotrophic factor, and improved cholinergic and monoaminergic transmission following 3-NPA intoxication. Taken together, RSe co-administration may prevent or delay the progression of HD and its associated impairments through its antioxidant, anti-inflammatory, anti-apoptotic, and neuromodulatory effects. 2019, Springer Science+Business Media, LLC, part of Springer Nature.