Browsing by Author "Kamel, Mohamed Salah"

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Anti-epileptic potential, metabolic profiling and in silico studies of the aqueous fraction from O cimum menthiifolium benth, family Lamiaceae
(Taylor and Francis Ltd, 2020-08) Zahran, Eman Maher; Usama, Ramadan; Abdel mohsen, Ahmed; Kolkeila, M Alaraby; Salem, Ezzat; Khalil, Yehia; Desoukey, Ahmed; Fouad, Mohamed Salah; Abdel mohsen, Usama Ramadan; Kolkeila, Ahmed; Salem, Mohammad Alaraby; Khalil, Hany Ezzat; Desoukey, Samar Yehia; Fouad, Ahmed; Kamel, Mohamed Salah
Get access Share icon Skip to Main Content Log in | Register Search in: Journal Natural Product Research Formerly Natural Product Letters Latest Articles 17 Views 0 CrossRef citations to date 0 Altmetric Short Communication Anti-epileptic potential, metabolic profiling and in silico studies of the aqueous fraction from O cimum menthiifolium benth, family Lamiaceae Eman Maher Zahran ,Usama Ramadan Abdelmohsen,Ahmed Kolkeila,M. Alaraby Salem,Hany Ezzat Khalil,Samar Yehia Desoukey, show all Received 04 Apr 2020, Accepted 03 Aug 2020, Published online: 18 Aug 2020 Download citation https://doi.org/10.1080/14786419.2020.1809396 CrossMark LogoCrossMark اختيار اللغة▼ Translator disclaimer Abstract The current study aimed to investigate the anti-epileptic potential of the ethanol extract and its different fractions from the Lamiaceous plant, Ocimum menthiifolium. The results revealed that the aqueous fraction with the latest onset of myoclonic convulsions (1095 ± 45**** s) was the most biologically active one. This was followed by LC-HR-MS-coupled metabolic profiling which led to dereplication of 8 compounds from that fraction. A molecular docking study was performed on the dereplicated compounds to discover the main responsible ones for the activity. The results highlighted Apigenin-7,4'-di-O-glucoside as the top scoring ligand with a possible mechanism of action involving the modulation of the voltage-gated sodium channel.
Chemical and biological studies on the soft coral Nephthea sp
(Royal Society of Chemistry, 2021-06) Abdelhafez, Omnia Hesham; Fahim, John Refaat; El Masri, Ramy R.; Salem, M. Alaraby; Desoukey, Samar Yehia; Ahmed, Safwat; Kamel, Mohamed Salah; Pimentel-Elardo, Sheila Marie; Nodwell, Justin R; Abdelmohsen, Usama Ramadan
Soft corals belonging to the family Nephtheidae have been appreciated as marine sources of diverse metabolites with promising anticancer potential. In view of that, the current work investigates the anti- proliferative potential of the crude extract, different fractions, and green synthesized silver nanoparticles (AgNPs) of the Red Sea soft coral, Nephthea sp. against a panel of tumor cell lines. The metabolic pool of the soft coral under study was also explored via an LC-HR-ESI-MS metabolomics approach, followed by molecular docking analysis of the characterized metabolites against the target proteins, EGFR, VEGFR, and HER2 (erbB2) that are known to be involved in cancer cell proliferation, growth, and survival. Overall, the n- butanol fraction of Nephthea sp. exhibited the highest inhibitory activities against MCF7 (breast cancer) and A549 (lung cancer) cell lines, with interesting IC50 values of 2.30 0.07 and 3.12 0.10 mg ml 1 , respectively, whereas the maximum growth inhibition of HL60 (leukemia) cells was recorded by the total extract (IC50 ¼ 2.78 0.09 mg ml 1 ). More interestingly, the anti-proliferative potential of the total soft coral extract was evidently improved when packaged in the form of biogenic AgNPs, particularly against A549 and MCF7 tumor cells, showing IC50 values of 0.72 0.06 and 9.32 0.57 mg ml 1 , respectively. On the other hand, metabolic profiling of Nephthea sp. resulted in the annotation of structurally diverse terpenoids, some of which displayed considerable binding affinities and molecular interactions with the studied target proteins, suggesting their possible contribution to the anti-proliferative properties of Nephthea sp. via inhibition of tyrosine kinases, especially the EGFR type. Taken together, the present findings highlighted the relevance of Nephthea sp. to future anticancer drug discovery and provided a base for further work on the green synthesis of a range of bioactive NPs from marine soft corals.
Chemical and biological studies on the soft coral Nephthea sp.
(Royal Society of Chemistry, 2021-06) Abdelhafez, Omnia Hesham; Fahim, John Refaat; El Masri, Ramy R; Salem, M. Alaraby; Desoukey, Samar Yehia; Ahmed, Safwat; Kamel, Mohamed Salah; Pimentel-Elardo, Sheila Marie; Nodwell, Justin R; Abdelmohsen, Usama Ramadan
Soft corals belonging to the family Nephtheidae have been appreciated as marine sources of diverse metabolites with promising anticancer potential. In view of that, the current work investigates the anti- proliferative potential of the crude extract, different fractions, and green synthesized silver nanoparticles (AgNPs) of the Red Sea soft coral, Nephthea sp. against a panel of tumor cell lines. The metabolic pool of the soft coral under study was also explored via an LC-HR-ESI-MS metabolomics approach, followed by molecular docking analysis of the characterized metabolites against the target proteins, EGFR, VEGFR, and HER2 (erbB2) that are known to be involved in cancer cell proliferation, growth, and survival. Overall, the n- butanol fraction of Nephthea sp. exhibited the highest inhibitory activities against MCF7 (breast cancer) and A549 (lung cancer) cell lines, with interesting IC50 values of 2.30 0.07 and 3.12 0.10 mg ml 1 , respectively, whereas the maximum growth inhibition of HL60 (leukemia) cells was recorded by the total extract (IC50 ¼ 2.78 0.09 mg ml 1 ). More interestingly, the anti-proliferative potential of the total soft coral extract was evidently improved when packaged in the form of biogenic AgNPs, particularly against A549 and MCF7 tumor cells, showing IC50 values of 0.72 0.06 and 9.32 0.57 mg ml 1 , respectively. On the other hand, metabolic profiling of Nephthea sp. resulted in the annotation of structurally diverse terpenoids, some of which displayed considerable binding affinities and molecular interactions with the studied target proteins, suggesting their possible contribution to the anti-proliferative properties of Nephthea sp. via inhibition of tyrosine kinases, especially the EGFR type. Taken together, the present findings highlighted the relevance of Nephthea sp. to future anticancer drug discovery and provided a base for further work on the green synthesis of a range of bioactive NPs from marine soft corals.