Browsing by Author "Huwait, Etimad"

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    Khalas date flavonoids inhibited cell viability, induced apoptosis and expression of the pro-autophagy LC3-B gene in human hepatocellular carcinoma cells (HepG2)
    (Taylor and Francis Ltd., 2022-11) AL-Ghamdi, Maryam Abdu; Alsulami, Rawyah Radi; Bakkar, Ashraf; Kumosani, Taha Abullah; Barrbour, Elie Kamil; Abulnaja, Khalid Omar; Huwait, Etimad; Moselhy, Said Salama
    Autophagy is a protective mechanism important in human dis- eases as cancer. We evaluated the impact of khalas date extract (KDE) (20-60 mg/mL) on cell viability, morphological changes, DNA fragmentation and gene expression of LC3B-II associated with autophagosome on HepG2 cell line. The GC/MS identification of KDE showed its high content of flavonoids including quercetin, myricetin, kaempferol and catechol. KDE reduced cell viability of HepG2 with IC50 (31.52 mg/mL). Cells treated with KDE showed two band of DNA fragments at (30 and 40 mg) indicating that KDE induced DNA damage and apoptosis in HepG2. The analysis RT-PCR data showed a 0.2-fold increase in the expression of LC3- B in the cells treated with KDE versus control. We concluded that, KDE flavonoids such as quercetin, myricetin kaempferol exhibited anticancer properties manifested by inhibition of HepG2 cell via- bility and induction of apoptosis and upregulation of the pro- autophagy LC3-B gene. ARTICLE HISTORY
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    Strigol1/albumin/chitosan nanoparticles decrease cell viability, induce apoptosis and alter metabolomics profile in HepG2 cancer cell line
    (Elsevier, 08/02/2021) Al-Malki, Abdulrahman L; Bakkar, Ashraf; Huwait, Etimad; Barbour, Elie K; Abulnaja, Kalid O; Kumosani, Taha A; Moselhy, Said S
    Biomedicine & Pharmacotherapy Volume 142, October 2021, 111960 Strigol1/albumin/chitosan nanoparticles decrease cell viability, induce apoptosis and alter metabolomics profile in HepG2 cancer cell line Author links open overlay panelAbdulrahman L.Al-MalkiabcAshrafBakkardEtimad A.HuwaitaElie K.BarbourabeKalid O.AbulnajaabcTaha A.KumosaniabfSaid S.Moselhyg a Biochemistry Department, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia b Experimental Biochemistry Unit, King Fahd Medical Research Centre, King Abdulaziz University, Saudi Arabia c Bioactive Natural Products Research Group, King Abdulaziz University. Jeddah, Saudi Arabia d Modern Sciences and Arts University (MSA), 6th October, Giza, Egypt e Director of R and D Department, Opticon Hygiene Consulting, Oechsli 7, 8807 Freienbach, Switzerland f Production of Bio-products for Industrial Applications Research Group, King Abdulaziz University g Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt Received 16 March 2021, Revised 21 July 2021, Accepted 22 July 2021, Available online 2 August 2021. crossmark-logo https://doi.org/10.1016/j.biopha.2021.111960 Get rights and content Under a Creative Commons licenseopen access Highlights • Novel strigol nanoparticles (S/A/CNP) exhibit antiproliferative activity against HepG2 cells through dose dependent. • The S/A/CNP significantly reduces Krebs cycle intermediate and decrease glutamine level as energy source substrate. • The S/A/CNP induce morphological changes and trigger pro-apoptotic effects in HepG2. • The S/A/CNP reduces spermine and spermidine and inhibit DNA replication. Abstract Hepatocellular carcinoma is one of the most common causes of cancer-related deaths globally. Bioavailable, effective and safe therapeutic agents are urgently needed for cancer treatment. This study evaluated the metabolomics profiling, anti-proliferative and pro-apoptotic effects of strigol/albumin/chitosan nanoparticles (S/A/CNP) on HepG2 cell line. The diameter of S/A/CNP was (5 ± 0.01) nm. The IC50 was 180.4 nM and 47.6 nM for Strigol1 and S/A/CNP, respectively, after incubation for 24 h with HepG2 cells. By increasing the concentration of S/A/CNP, there was chromatin condensation, degranulation in the cytoplasm and shrinking in cell size indicating pro-apoptotic activity. Metabolomics profiling of the exposed cells by LC/MS/MS revealed that S/A/CNP up-regulated epigenetic intermediates (spermine and spermidine) and down-regulated energy production pathway and significantly decreased glutamine (P < 0.001). These findings demonstrated that S/A/CNP has anti-proliferative, apoptotic effects and modulate energetic, and epigenetic metabolites in the hepatocellular carcinoma cell line (HepG2).