Browsing by Author "Heba H. Awad"

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    Innovative natural anti-aging nanofiber facial mats: Harnessing quality by design and artificial intelligence for sustainable skincare solutions
    (Editions de Sante, 2025-06-02) Marwa H.S. Dawoud; Abdelrahman E. Gharieb; Ahmed H.M. Hassan; Omaima M.B. Hendawy; Heba H. Awad
    The growing emphasis on skin health among aging population has sparked attention to combat aging. Cosmeceutical products with natural ingredients provide safe, effective, and sustainable formulation with demand for clean and organic skincare. Pomegranate and collagen have emerged as promising antiaging agents, which could be easily incorporated into electrospun biodegradable nanofibers (ESN), especially if incorporated with chitosan. This study aims to formulate ESN loaded with these natural antiaging agents, using Quality by Design (QbD), complemented by artificial intelligence (AI) to predict the nanofiber diameter. A complete risk assessment study was conducted, where screening of the electrospinning solution for viscosity, surface tension, pH, and electrical conductivity to ensure optimal electrospinning conditions. The solution showed an optimal viscosity of 330.87 mPa s, surface tension of 41.76 dyne/cm, and electrical conductivity of 10004.78 μs/cm. The electrospinning process was meticulously optimized, with a flow rate of 0.2 mL/h, a voltage of 25 kV, 40 % humidity, and a needle-to-collector distance of 15 cm. An AI model was employed to predict the nanofiber diameter, demonstrating exceptional predictive accuracy. The formulated pomegranate and collagen nanofibers mat was tested in-vivo and showed significant antioxidant, anti-inflammatory, and tissue-regenerative properties, restoring SOD and collagen levels, reducing MMP-1, COX-2, and PGE-2 levels, and effectively attenuating NF-κB activation, highlighting the therapeutic potential of the formulated mats for managing skin aging. This study provides novel insights into the integration of QbD and AI for the development of natural product-based formulations, paving the way for their application in advanced pharmaceutical and cosmeceutical systems. © Elsevier B.V
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    Optimizing nutraceutical-loaded trehalosomes in-situ gel for diabetic cataract management: Comprehensive in vitro and in vivo evaluations
    (Editions de Sante, 2024-11-03) Aya Hussein; Maha El-Kayal; Rehab Nabil Shamma; Heba H. Awad; Nihal Farid Younes
    This study aimed to develop Baicalin-loaded trehalosomes in situ gel (BA-THs-ISG) for the treatment of cataract, a degenerative eye condition that requires innovative approaches to enhance visual acuity and quality of life. A 33 D-optimal design was implemented for the preparation and statistical optimization of BA-THs. Various in-vitro studies were conducted to evaluate the features of the optimized formula, which was subsequently loaded into an optimum ISG to sustain the release pattern. To assess the therapeutic efficacy of BA-THs-ISG, an in-vivo study was conducted using a streptozotocin (STZ) induced diabetic cataract model. The results demonstrated that the optimized formula of desirability function of 0.878 had a particle size (PS) of 237.9 ± 2.87 nm, a polydispersity index (PDI) of 0.27 ± 0.03, a zeta potential (ZP) of −27.31 ± 1.27 mV, an entrapment efficiency (EE%) of 74.32 ± 1.08 % and sustained drug release over 6 h. After being loaded into ISG, BA-THs-ISG exhibited a gelation temperature of 35.00 ± 0.30 °C and a gelation time of 49 ± 2.63 s, meeting the intended criteria. Moreover, it effectively maintained drug release for a duration of 12 h. Compared to the BA solution in the in-vivo studies, BA-THs-ISG significantly reduced malondialdehyde content and enhanced superoxide dismutase and glutathione peroxidase activities without any irritation potential. Therefore, BA-THs-ISG is inferred to be a highly effective option for improving cataract symptoms.