Browsing by Author "Hassan N."

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Comparative study of new spectrophotometric methods; An application on pharmaceutical binary mixture of ciprofloxacin hydrochloride and hydrocortisone
(Sociedad Chilena de Quimica, 2013) Lotfy H.M.; Hassan N.; Elgizawy S.M.; Saleh S.S.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini Street; 11562 Cairo; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Assuit University; 71515 Assuit; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Egypt
Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of Ciprofloxacin hydrochloride (CIP) and Hydrocortisone (HYD) in their pure form and pharmaceutical dosage form. Two new spectrophotometric methods were applied: extended ratio subtraction (EXRSM) and ratio difference (RDSM) methods. The results were compared to three well-established methods: Mean centering of ratio spectra method (MCR), Isoabsorpative point spectrophotometric method (IsoM) and Absorbance ratio method (ARM). The linearity range for the spectrophotometric methods was found to be (2-14 ?g/mL) and (1-14 ?g/mL) for CIP and HYD respectively. The selectivity of the developed methods was investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The results obtained from the proposed methods were statistically compared using one-way analysis of variance (ANOVA) where no significant difference was observed between the new (EXRSM and RDSM) and the well-established methods which prove their validity for the analysis of this binary mixture. The methods were validated as per ICH guidelines regarding accuracy, precision, repeatability and robustness; which were found to be within the acceptable limits. The new methods were simple, sensitive and don't need a special program, so they could be easily applied as alternative methods to LC methods in quality control laboratories lacking the required facilities for those techniques.
Comparative study of RP-HPLC versus TLC-spectrodensitometric methods applied for binary mixtures of fluoroquinolones and corticosteroids
(Akademiai Kiado Rt., 2014) Elgizawy S.; Hassan N.; Lotfy H.; Saleh S.; Assuit University; Analytical Chemistry Department; Faculty of Pharmacy; 71515 Assuit; Egypt; Cairo University Analytical Chemistry Department; Faculty of Pharmacy; Kasr-El Aini Street; 11562 Cairo; Egypt; October University for Modern Sciences and Arts (MSA); Analytical Chemistry Department; Faculty of Pharmacy; 11787 6th October City; Egypt
Reversed phase high-performance liquid chromatography (RP-HPLC) and thin-layer chromatography (TLC)-spectrodensitometric methods have been developed and validated for the separation and quantitation of two binary mixtures: Ofloxacin (OFX) and dexamethasone (DXM) in eye preparation; ciprofloxacin hydrochloride (CIP) and hydrocortisone (HYD) in ear preparation. The linearity ranges of RP-HPLC methods were found to be (2.5-45 ?g mL-1) for OFX, (2.5-50 ?g mL-1) for DXM and (1-8 ?g mL-1) for both CIP and HYD. The percentage recoveries/relative standard deviation (RSD) were found to be 100.36/1.38, 100.13/1.49, 99.98/0.61 and 100.28/1.27, respectively. The linearity ranges of TLC-spectrodensitometric methods were found to be (0.5-2 ?g band-1), (0.5-3.5 ?g band-1), (0.2-1.6 ?g band-1), and (0.6-2 ?g band-1) for OFX, DXM, CIP, and HYD, respectively. The percentage recoveries/RSD were found to be 99.98/1.06, 99.93/1.18, 99.74/1.27, and 99.94/1.54, respectively. A comparative study was conducted to show the advantages of the proposed methods which showed that the TLC-spectrodensitometric methods were simpler, more sensitive, and economic, while RP-HPLC methods were more precise and robust. The methods were validated in compliance with the ICH guidelines and were successfully applied for determination of the selected drugs in their laboratory-prepared mixtures and commercial dosage forms.