Browsing by Author "Hassan, Osama S"

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    COMPARATIVE EVALUATION OF ESCHERICHIA COLI AND BACILLUS CEREUS PRODUCTION POTENTIALS OF ACETIC ACID UNDER OPTIMIZED FERMENTATION CONDITIONS
    (2016-05) Hassan, Osama S; Abo El Magid, Shaimaa M; Shaker, Lmaiaa S; Murad, Aya A; William, Hala F.
    Escherichia coli remains of the best-established production organism in industrial biotechnology. Bacillus Cereus is a recognized human pathogenic bacterium, which can colonize in the human intestine and frequently implicated in cases of foodborne poisoning. The goal of this work focused on production of acetic acid for it's important and benefit uses in different industrial products. The aerobic fermentation was run on media containing glucose and supplied with some minerals to increase the growth rate. The uptake of glucose was converted into biomass and acetic acid. The results of this work indicated that, the using of glucose and minerals increased the growth rate. The dry cell weight was 3.5 g/l , 2.9 g/l and the acetic acid concentration was 30 mg/ml , 4.33 mg/ml in case of E. coli and B. Cereus respectively. These data indicated that the fermentation culture of E. coli can be industrially used for production of a high yield of acetic acid than B. Cereus.
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    Controlled Study of Application of Bee Products as New Medicine Against Breast and Lung Cancer.
    (2016) Mohamed, Aly F.; El-Fiky, Abir A.; Hassan, Osama S; El-Megharbel, Nada A
    Bee venom has special pharmacological activity through its complex protein structure which contains enzymes and peptides. Melittin is the bee venom’s major component constituting about 50% of its content. Recent studies showed that bee venom has a huge impact in the treatment of tumors due to synergistic antitumor effect of bee venom melittin and phospholipase A2. Propolis is a substance produced by bees from the resin collected from trees and act as an anticancer agent. The major antitumor activity of propolis depends on its main components CAPE and chrysin. This study aimed at estimating the anti-cancer potentials of Propolis, Egyptian and Georgian bee venom on breast cancer cell line MCF-7 and Lung cancer cell line A549. The Egyptian bee venom showed higher toxicity on both cells than the Georgian bee venom. After the 24 hr treatment with Egyptian bee venom and Propolis, the results showed significance inhibitory effects on cells proliferation and induced morphological changes. The gene expression level of pro-apoptotic gene as p53 and anti-apoptotic gene as Bcl-2 were examined in both cell lines after 24 hr of treatment. Data recorded revealed that treatment with bee venom and Propolis showed clear anticancer potentials based on the up / down regulation of pro-apoptotic and anti-apoptotic gene.