Browsing by Author "Hassan, Emad M"

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Biochemical and Pharmacological Prospects of Citrus sinensis Peel
(Elsevier BV, 2022-07) Abou Baker, Doha H; Ibrahim, Bassant M.M; Abdel-Latif, Yasmin; Hassan, Nabila S; Hassan, Emad M; El Gengaihi, Souad
Gastric ulcer and hepatotoxicity due to irrational drug overuse are two of the most serious conditions associated with inflammation and oxidative stress that affect the digestive system. This study aimed to experimentally evaluate the hepatoprotective/gastroprotective effects of aqueousandbutanol citrus peel extracts and hesperidin in rat models of ulcer and hepatotoxicity. Acute toxicity study was performed for determiningthe safe dose of citrus extracts toanalyze efficacy. In the experiments on hepatoprotective and gastroprotective effects, rats were classified into nine groups in each experiment: (1)negative control, (2) positive control hepatotoxic model with paracetamol (640mg/kg)/gastric ulcer model:ethanol 70%(1ml), (3)reference hepatoprotective:silymarin (25 mg/kg)/gastroprotective:ranitidine (50mg/kg), and (4–9) groups treated for 2 weeks before induction of each disease with either citrus aqueous or butanol extracts or hesperidin (125–250 mg/kg). Drugs, ethanol, or tested compounds were administered orally. The levels of biochemical parameters,suchas AST,ALT,NO,MDA,CRP, and ILβ6, were significantly reduced,but CAT level was increased.Postmortem examination of liver and stomach tissues of treated animalsrevealed marked improvement compared with positive control animals. Hesperidin exerted the best hepatoprotective, antioxidant, anti- inflammatory, and gastroprotective effects, followed by butanol and then aqueous citrus peel extracts.
Biochemical and pharmacological prospects of Citrus sinensis peel
(Elsevier BV, 2022-08) Abou Baker, Doha H; Ibrahim, Bassant M.M.; Abdel-Latif, Yasmin; Hassan, Nabila S.; Hassan, Emad M; El Gengaihi, Souad
Gastric ulcer and hepatotoxicity due to irrational drug overuse are two of the most serious conditions associated with inflammation and oxidative stress that affect the digestive system. This study aimed to experimentally evaluate the hepatoprotective/gastroprotective effects of aqueous and butanol citrus peel extracts and hesperidin in rat models of ulcer and hepatotoxicity. Acute toxicity study was performed for determining the safe dose of citrus extracts to analyze efficacy. In the experiments on hepatoprotective and gastroprotective effects, rats were classified into nine groups in each experiment: (1) negative control, (2) positive control hepatotoxic model with paracetamol (640 mg/kg)/gastric ulcer model:ethanol 70% (1 ml), (3)reference hepatoprotective:silymarin (25 mg/kg)/gastroprotective:ranitidine (50 mg/kg), and (4–9) groups treated for 2 weeks before induction of each disease with either citrus aqueous or butanol extracts or hesperidin (125–250 mg/kg). Drugs, ethanol, or tested compounds were administered orally. The levels of biochemical parameters, such as AST, ALT, NO, MDA, CRP, and ILβ6, were significantly reduced, but CAT level was increased. Postmortem examination of liver and stomach tissues of treated animals revealed marked improvement compared with positive control animals. Hesperidin exerted the best hepatoprotective, antioxidant, anti-inflammatory, and gastroprotective effects, followed by butanol and then aqueous citrus peel extract
Pharmacological and metabolomic profiles of Musa acuminata wastes as a new potential source of anti-ulcerative colitis agents
(Nature Publishing Group, 2022-06-22) Mohammed, Mona A; Ibrahim, Bassant M. M; Abdel-Latif, Yasmin; Hassan, Azza H; El Raey, Mohamed A; Hassan, Emad M; El-Gengaihi, Souad E
Musa acuminata (MA) is a popular fruit peels in the world. Non-food parts of the plant have been investigated for their antioxidant and anti-ulcerative colitis activity. Metabolomic approaches were found to be informative as a screening tool. It discovered diferent metabolites depending on statistical analysis. The antioxidant activity content was measured by colorimetric method. Seventy six investigated metabolites were observed. The identities of some of these markers were confrmed based on their MS2 fragmentation and NMR spectroscopy. These include: cinnamic acid and its dimer 2-hydroxy-4-(4-methoxyphenyl)-1H-phenalen-1-one beside; gallic acid and favonoids; quercetin, quercetin-3-O-β-d-glucoside, luteolin-7-O-β-d-glucopyranoside. GC/MS analysis of MA peels essential oil led to identifcation of 37 compounds. The leaves, pseudostem and fruit peels extracts were tested for their safety and their anti-ulcerative colitis efcacy in rats. Rats were classifed into: normal, positive, prednisolone reference group, MA extracts pretreated groups (250–500 mg/kg) for 2 weeks followed by induction of ulcerative colitis by per-rectal infusion of 8% acetic acid. Macroscopic and microscopic examinations were done. Infammatory markers (ANCA, CRP and Ilβ6) were measured in sera. The butanol extracts showed good antioxidant and anti-infammatory activities as they ameliorated macroscopic and microscopic signs of ulcerative colitis and lowered the infammatory markers compared to untreated group. MA wastes can be a potential source of bioactive metabolites for industrial use and future employment as promising anti-ulcerative colitis food supplements.