Browsing by Author "Hashem A.M."

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Antimicrobial activities against biofilm formed by Proteus mirabilis isolates from wound and urinary tract infections
(2012) Wasfi R.; Abd El-Rahman O.A.; Mansour L.E.; Hanora A.S.; Hashem A.M.; Ashour M.S.; Department of Microbiology; Faculty of Pharmacy; University for Modern Sciences and Arts; Giza; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Suez Canal University; Ismailia; Egypt; Department of Microbiology and Immunology; Faculty of Pharmacy; Cairo University; Cairo; Egypt
Background: Bacterial species are capable of living as biofilm and/or planktonic forms. There is increasing evidence for the role of bacterial biofilm in various wound and urinary tract infections (UTIs). The aim of the present study was to evaluate the ability of the bacteria, isolated from urinary tract infections (UTIs) and wound infections, to form biofilm and correlate the role of biofilm with their antimicrobial resistance. Materials and Methods: All the isolated bacteria were screened for their ability to form biofilm using the microtitre plate method. Results: Wound isolates of Staphylococcus aureus and Enterobacter sp. had more biofilm forming capacity than the UTI isolates. Proteus mirabilis isolates were among the strongest biofilm forming bacteria and were chosen for antimicrobial study. In sub-MIC concentrations of antimicrobial agents used, ciprofloxacin was found to be the most effective in decreasing biofilm formation. On the other hand, ceftriaxone and ciprofloxacin were effective in partial removal of preformed biofilm biomass. Conclusion: Ciprofloxacin was more effective in killing bacterial cells especially at high antimicrobial concentrations that could be reached in urine levels and can be used in impregenating catheters.
Polyphenols in Ammania auriculata: Structures, antioxidative activity and cytotoxicity
(Govi-Verlag Pharmazeutischer Verlag GmbH, 2014) Nawwar M.A.; Youb N.A.; El-Raey M.A.; Zaghloul S.S.; Hashem A.M.; Mostafa E.S.; Eldahshan O.; Werner V.; Becker A.; Haertel B.; Lindequist U.; Linscheid M.W.; Department of Phytochemistry and Plant Systematics; National Research Center; Dokki Cairo; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; October University for Modern Sciences and Arts; 6th October City; Egypt; Institute of Pharmacy; Pharmaceutical Biology; Ernst-Moritz-Arndt-Universit�t; Greifswald; Mecklenburg-Vorpommern; Germany; Department of Chemistry; Laboratory of Applied Analytical and Environmental Chemistry; Humboldt -University; Berlin; Germany
Chemical and biological investigations of the extract of Ammania auriculata (Lytheraceae) resulted in the identification of eight polyphenols (1 - 8) for the first time from this plant, including the gallotannin, 2,3,6-tri-O-galloyl-(?,?)-4C1-glucopyranose (8), for which 1D and 2D-NMR spectra were recorded and assigned for the first time. The structures of all isolates (1 - 8) were elucidated by conventional methods, spectroscopic analysis, including 1D and 2D NMR, and by HR-ESIMS as well. All of the isolated compounds were evaluated for their antioxidant activities, determined by the DPPH and ORAC methods and for their cytotoxicity against the keratinocyte cell line HaCaT using the neutral red assay (NRU) and cell cycle analysis. Compounds 1, 3, 4, 5, and 6 significantly inhibited reactive oxygen species production with ED50 values between 3.22 and 9.79 ?g/ml. Compounds 1, 3, 4, and 5 showed cytotoxic activity against HaCaT cells with IC50 values between 30.7 and 84.1 ?g/ml. The new galloyl glucose (8) was found not cytotoxic. Ellagitannins, 2,3-hexahydroxy-(?/?)-glucopyranose (1) and 1-O-galloyl 2,3-hexahydroxy-(?)-glucopyranose (5) possess remarkable antioxidative and comparably weak cytotoxic activity.