Browsing by Author "Hammam O."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Differential expression of RAGE, EGFR and Ki-67 in primary tumors and lymph node deposits of breast carcinoma
    (Wiley-VCH Verlag, 2018) Aboushousha T.; Hammam O.; Safwat G.; Eesa A.; Ahmed S.; Esmat M.E.; Helmy A.H.; Department of Pathology; Cairo university; Cairo; Egypt; Faculty of Biotechnology; October University for Modern Sciences and Arts; Cairo university; Cairo; Egypt; Department of Pathology; Faculty of Medicine; Cairo university; Cairo; Egypt; Department of Surgery; Theodor Bilharz Research Institute; Cairo university; Cairo; Egypt
    Background: Breast cancer is a complex disease that results from the inheritance of a number of susceptible genes. Intensive search wok was conducted world-wide on molecular bases of breast cancer in order to achieve the best therapeutic modalities; however, breast cancer still remains a challengeable task. It is very important to determine if the biological parameters in metastatic regional lymph nodes are similar to that in the primary breast cancer because therapy is indicated for patients with synchronous metastatic regional lymph nodes of breast cancer. Difference in therapeutic response in cases of breast cancer may be assumed partially to variability in the biological behavior of tumor tissue in primary breast cancer and lymph node metastasis. Aim: Our aim is to evaluate any variability in the expression of three types of tissue markers in both the primary breast tumors and corresponding axillary lymph nodes in order to expect the targeted therapeutic effect on both sites. Material and Methods: Three markers from different categories; RAGE, EGFR and Ki-67 were immunohistochemicalyl studied for their expression in biopsy specimens from primary breast tumors and their corresponding axillary lymph nodes. Results: There was a statistically significant difference in the expression of these markers between benign and malignant breast lesions.Although we found some differences in the expression of the three studied markers between primary breast cancer and corresponding axillary lymph nodes, yet these variations were mostly not statistically significant. Conclusion: Our findings support the validity of anti-RAGE and anti-EGFR therapy for treatment of both primary and nodal metastatic breast cancer in immunopositive cases. � 2018 Asian Pacific Organization for Cancer Prevention.
  • Thumbnail Image
    Item
    Overview of MDM2 and B-RAF expression in gastric lesions
    (Springer, 2018) Aboushousha T.; Helal N.; Hammam O.; Ibrahim M.; Khaled S.; Mostafa A.; Anas A.; Department of Pathology; Theodor Bilharz Research Institute; Imbaba; Giza; Egypt; Faculty of Biotechnology; October University of Modern Sciences and Arts; Giza; Egypt; Department of Surgery; Theodor Bilharz Research Institute; Imbaba; Giza; Egypt; Department of Hepato-Gastroenterology; Theodor Bilharz Research Institute; Giza; Egypt
    BACKGROUND: Globally, gastric cancer (GC) it is the fourth most common cancer and the third cause of cancer-related deaths. Overexpression of MDM2 and B-RAF appeared to be increased in malignancy and associated with poor prognosis in several human tumours, but their role in gastric cancer remains controversial. AIM: We had investigated the immunohistochemical expression of MDM2 and B-RAF in 136 gastric lesions with/without H. pylori association. MATERIAL AND METHODS: Studied specimens include chronic gastritis (32), intestinal type GC (70), diffuse GC (22) and gastrointestinal stromal tumours (GIST) (12). RESULTS: MDM2 expression increased significantly in intestinal GC compared to other groups (p < 0.001), while B-RAF expression increased significantly in GIST compared to other groups (p < 0.001). H. pylori increased expression of MDM2 in intestinal GC cases but did not affect B-RAF expression. MDM2 expression correlated with high grade of tumor differentiation (p < 0.001), deep invasion (p < 0.05), nodal metastases (p < 0.05) and distant metastases (p < 0.1) in intestinal GC, while B-RAF expression did not correlate with TNM stage (p < 0.1). CONCLUSION: MDM2 up-regulation was more frequent in intestinal GC, while B-RAF up-regulation was more frequent in GIST compared to other groups; MDM2 expression in intestinal GC was correlated with H. pylori association, high grade of differentiation, deep invasion, nodal and distant metastases, meanwhile, B-RAF expression was correlated with high-grade intestinal GC but did not correlate with H. pylori or TNM stage. The possible role of both MDM2 and B-RAF in predicting progression of gastric tumours and prognosis deserves further investigations. 2018 Tarek Aboushousha, Noha Helal, Olfat Hammam, Manar Ibrahim, Samar Khaled, Amr Mostafa, Amgad Anas.