Browsing by Author "Hafez M.M."

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Comparative study on serodiagnostic techniques of Brucella infection in Egypt
(2012) Basyony A.F.; Aboulwafa M.M.; Hafez M.M.; Abou Gazia K.A.; Department of Microbiology and Immunology; Faculty of Pharmacy; Modern Sciences and Arts University; Egypt; Microbiology and Immunology Department; Faculty of Pharmacy; Ain Shams University; Egypt; Department of Microbiology and Immunology; Animal Reproduction Research Institute; Agriculture Research Center; Egypt
Background: Brucellosis is a zoonotic disease that affects wild and domestic animals causing a decrease in reproductive efficiency and abortion and can be transmitted to human. The incidence of human disease is closely tied to the prevalence of infection in animals and considered as an important health problem in Egypt. Methods and Findings: In this study, blood specimens from 68 patients that showed clinical signs and/or history of brucellosis and from different investigated animals (76 buffalo, 145 cattle and 191 sheep) were collected and serodiagnosed for Brucella infection. The sera of these blood specimens were first screened by rose bengal plate test (RBPT) and those giving positive reaction were retested by the standard tube agglutination test (SAT), EDTA modified SAT and rivanol test to determine their titers. The results for clinical specimens showed that 89.70%, 82.35%, 66.18% and 58.82% were positive using RBPT, SAT, EDTA modified SAT and rivanol test, respectively. The respective percentages of brucellosis in buffalo were 44.70%, 43.42%, 43.42% and 43.24%; while the respective percentages of brucellosis in cattle were 46.90%, 43.45%, 39.31% and 37.93%. In addition, serological examination of 191 sheep revealed that 60.20%, 56.54%, 53.40% and 51.83% were positive using RBPT, SAT, EDTA modified SAT and rivanol test, respectively. Conclusion: The results give clear evidence for: (i) the real picture of brucellosis surveillance among human cannot be reflected using single serodiagnostic test, (ii) In comparison to human, serodiagnosis of Brucella among animals is less dependent on test type and such dependency took the order sheep > cattle > buffalo, (iii) serodiagnosis of Brucella among buffalo had nearly no dependency on test type. � Under License of Creative Commons Attribution 3.0 License.
Management of cardiac fibrosis in diabetic rats; The role of peroxisome proliferator activated receptor gamma (PPAR-gamma) and calcium channel blockers (CCBs)
(2011) Mohamad H.E.; Askar M.E.; Hafez M.M.; Department of Biochemistry; Faculty of Pharmacy; Zagazig University; Zagazig; Egypt; Department of Biochemistry; Faculty of Pharmacy; October for Modern Science and Arts University (MSA); Egypt
Background: Diabetes mellitus (DM) and hypertension (HTN) are accused of being responsible for the development of the cardiac fibrosis due to severe cardiomyopathy. Methods. Blood glucose (BG) test was carried out, lipid concentrations, tumor necrosis factor alpha (TNF-?), transforming growth factor beta (TGF-?), matrix metalloproteinase (MMP-2), collagen-I and collagen-III were measured in male Albino rats weighing 179-219 g. The rats were divided into five groups, kept on either control diet or high fat diet (HFD), and simultaneously treated with rosiglitazone (PPAR-gamma) only for one group with 3 mg/kg/day via oral route for 30 days, and with rosiglitazone and felodipine combination for another group with 3 mg/kg/day and 5 mg/kg/day, respectively via oral route for 30 days. Results: Diabetic hypertensive (DH) rats which fed on a HFD, injected with streptozotocin (STZ) (i.p.) and obstruction for its right kidney was occurred develop hyperglycemia, hypertension, cardiac fibrosis, hypertriglyceridemia, hypercholesterolemia, increased TNF-?, increased TGF-?, decreased MMP-2, increased collagen-I and increased collagen-III, when compared to rats fed on control diet. Treating the DH rats with rosiglitazone only causes a significant decrease for BG levels by 52.79%, triglycerides (TGs) by 24.05%, total cholesterol (T-Chol) by 30.23%, low density lipoprotein cholesterol (LDL-C) by 40.53%, TNF-? by 20.81%, TGF-? by 46.54%, collagen-I by 48.11% and collagen-III by 53.85% but causes a significant increase for MMP-2 by 272.73%. Moreover, Treating the DH rats with rosiglitazone and felodipine combination causes a significant decrease for BG levels by 61.08%, blood pressure (BP) by 16.78%, TGs by 23.80%, T-Chol by 33.27%, LDL-C by 45.18%, TNF-? by 22.82%, TGF-? by 49.31%, collagen-I by 64.15% and collagen-III by 53.85% but causes a significant increase for MMP-2 by 290.91%. Rosiglitazone alone failed to decrease the BP in DH rats in the current dosage and duration. Conclusion: Our results indicate that the co-existence of diabetes and hypertension could induce cardiomyopathy which could further result in cardiac fibrosis, and that combination treatment with rosiglitazone and felodipine has a great protective role against the metabolic abnormalities, meanwhile, the treatment with rosiglitazone alone has a protective role with a minimal effect against these abnormalities and has no effect on decreasing BP in these cases which may lead to coronary artery diseases (CADs) in future. � 2011 Mohamad et al; licensee BioMed Central Ltd.
The Potential Role of Zinc Oxide Nanoparticles in MicroRNAs Dysregulation in STZ-Induced Type 2 Diabetes in Rats
(Humana Press Inc., 2019) Othman M.S.; Hafez M.M.; Abdel Moneim A.E.; B.Sc. Department; Preparatory Year College; University of Ha�il; Hail; Saudi Arabia; Faculty of Biotechnology; MSA University; Giza; Egypt; Biochemistry Department; Faculty of Pharmacy; Ahram Canadian University (ACU); Giza; Egypt; Zoology and Entomology Department; Faculty of Science; Helwan University; Cairo; Egypt
Diabetes mellitus (DM) is a group of metabolic disorders that are characterized by a loss of glucose homeostasis and insufficiency in production or action of insulin. Development of newly antidiabetic molecules using a variety of organic compounds and biomolecules has been in practice for a long time. Recently, nanomaterials are also being used in antidiabetic studies for their unique properties. In this context, zinc nanoparticles have drawn attention due to the relationship between diabetes and imbalance of zinc homeostasis. Few studies have attempted to investigate the effect of zinc oxide nanoparticles (ZON) in microRNA dysregulations in diabetes. To evaluate the therapeutic effect of ZON on streptozotocin (STZ)-induced diabetic rats as well as its role in microRNA dysregulations. Diabetes was induced in rats by 60�mg/kg body weight (bwt) of STZ and then treated with ZON (5�mg/kg bwt) for 15 consecutive days. The levels of glucose, insulin, oxidative stress markers, and microRNAs expression were measured in liver and pancreas tissues. Intraperitoneal injection of 60�mg/kg bwt of STZ to Wistar rats caused significant decreases in the body weight and Zn contents of pancreas, liver, and kidney. Also, STZ injection increased the blood glucose level and oxidative stress (lipid peroxidation (LPO) and nitric oxide (NO). Meanwhile, STZ decreased blood insulin and pancreatic anti-oxidants. STZ also resulted in ? cell dysfunction and destruction and altered the expression of certain pancreatic and liver microRNAs. ZON treatment for 15�days, at a dose of 5�mg/kg bwt resulted in marked improvements in the blood insulin, glucose tolerance, and structure and function of the pancreatic ? cells. Furthermore, ZON administration reduced LPO and NO, and increased the levels of enzymatic and non-enzymatic anti-oxidants in STZ-induced diabetic rats. It was found also that ZON specifically regulated the expression of pancreatic and liver microRNAs that involved in diabetes development. The obtained results revealed that ZON is a promising antidiabetic agent. The antidiabetic effect of ZON was partially mediated by restoring the oxidants/antioxidants balance and by modulating the alerted microRNAs. � 2019, Springer Science+Business Media, LLC, part of Springer Nature.