Browsing by Author "HI Habib, Ibrahim"

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    Simultaneous Spectrophotometric Determination of Diflunisal and Diclofenac Sodium in Pharmaceutical Dosage Form Using Chemometric Methods
    (Journal of Pharmaceutical Research International, 2016) HI Habib, Ibrahim; Rizk, Mohamed; Mohamed, Dalia; Mowaka, Shreen; Th El-Eryan, Rasha
    Aims: Is the simultaneous determination of Diflunisal and Diclofenac sodium in pharmaceutical preparations. Study Design: Four spectrophotometric methods (Classical Least Squares, Iterative Target Transformation Factor Analysis, Principal Component Regression and Partial Least Squares) will be described and evaluated for the simultaneous determination of the two drugs. In these techniques, the concentration data matrix was prepared according to the full factorial experimental design of three levels and two components in mixtures. Place and Duration of Study: Microanalytical Chemistry Laboratory, Applied Organic Chemistry Department, National Research Centre, Dokki, Giza, Egypt, between January 2016 and Marsh 2016. Methodology: Using 2.5 mM NaOH solution as solvent, the corresponding absorbance data matrix was measured over the wavelength range of 220 – 400 nm with the intervals of Δλ= 1 nm, then regression was obtained by using the absorbance data matrix and the concentration data matrix for the prediction of the unknown concentrations of Diflunisal and Diclofenac sodium in their mixture. The procedure did not require any chemical separation step or prior graphical treatment of the overlapped spectra. Results: The calibration range was found linear over 2.5 – 20 μg/mL for Diflunisal and Diclofenac sodium through the Partial Least Squares method. Conclusion: The Partial Least Squares method was selected and validated on both authentic mixtures and pharmaceutical preparations. The accuracy and precision of the methods were assessed and compared with each oth
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    Square Wave Voltammetric Determination of Ropinirole HCl in Bulk, Dosage Forms and Biological Samples on Carbon Paste Electrode
    (Journal of Pharmaceutical Research International, 2016) HI Habib, Ibrahim; Rizk, Mohamed; Mohamed, Dalia; Mowaka, Shereen; Th El-Eryan, Rasha
    Aim: Voltammetric determination for of Ropinirole HCl. Study: We will study the behaviour of Ropinirole HCl on the carbon paste electrode. So several factors such as pH, type of pasting oil, pulse amplitude and scan rate were studied to optimize the condition for voltammetric determination of drug. Place and Duration of Study: Microanalytical Chemistry Laboratory, Applied Organic Chemistry Department, National Research Centre, Dokki, Giza, Egypt, between August 2015 and December 2015. Methodology: Square wave voltammetry (SWV) was employed in order to determine Ropinirole in bulk powder and plasma in a voltammetric cell containing 10 mL of 0.1 mole L-1 sulphuric acid as supporting electrolyte. After every aliquot addition, the solution was stirred for 30 s at 2000 rpm, rested for 10 s then SWV mode was ramped from +300 to +1600 mV with scan rate 100 mV s-1 and pulse amplitude 50 mV. The experiment was triplicated for every standard solution addition. Results: A good linearity was obtained over a range of (4.96x10-6 to 3.90x10-5 mol L-1) with mean recovery and relative standard deviation (RSD) values of 99.15% and 3.7%, respectively. The lower detection (LOD) and quantification (LOQ) limits were found to be (1.48x10-6 and 4.96x10-6 mol L-1) respectively at Square wave (SWV) mode. The accuracy and precision of the method were presented with inter and intra days determinations which were within acceptable limits. The method was applied successfully for determining the active ingredients in pharmaceutical preparations with mean percentage recoveries ± RSD of 98.38±3.1 and in spiked human plasma with the mean of recoveries ± RSD, 99.56±3.63%. Conclusion: An economic, accurate and precise electrochemical method has been developed and validated for the determination of Ropinirole HCl in bulk, dosage form and human plasma.