Browsing by Author "Ghorab D.M."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Insulin Mucoadhesive Liposomal Gel for Wound Healing: a Formulation with Sustained Release and Extended Stability Using Quality by Design Approach
    (Springer New York LLC, 2019) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts; (MSA University); Giza; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
    The present study deals with the formulation of topical insulin for wound healing with extended stability and sustained release, by applying quality by design concepts. Insulin has been promoted as a promising therapeutic wound healing agent. Topical formulation of insulin faced major problems, as it cannot be delivered safely to the wound with a controlled rate. Formulation of insulin-loaded vesicles in optimized bio-adhesive hydrogels has been explored to ensure a safe delivery of insulin to wounds in a controlled manner. Quality by design (QbD) was applied to study the effect of several critical process parameters on the critical quality attributes. Ishikawa diagram was used to identify the highest risk factors, which were screened by a fractional factorial design and augmented by Box�Behnken design. The optimized formula was incorporated into a mucoadhesive gel, which was further subjected to stability and clinical studies. An optimized formula was obtained with a particle size of 257.751�nm, zeta potential ? 20.548�mv, 87.379% entrapment efficiency, and a release rate of 91.521�?g/cm2/h. The results showed that liposomal insulin remained stable for 6�months in aqueous dispersion state at 4�C. Moreover, the release was sustained up to 24�h. The clinical study showed an improvement in the wound healing rate, 16 times, as the control group, with magnificent reduction in the erythema of the ulcer and no signs of hypoglycemia. Insulin-loaded liposomal chitosan gel showed a promising drug delivery system with high stability and sustained release. � 2019, American Association of Pharmaceutical Scientists.
  • Thumbnail Image
    Item
    Response surface optimization and in-vitro evaluation of sustained release topical insulin liposomal spray for wound healing
    (Open Science Publishers LLP Inc., 2018) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
    Chronic wounds are considered a major health care concern, that represents a life-threatening problem worldwide. Insulin has proven its great efficiency as a wound healing agent, especially with diabetic ulcers. However; insulin suffers degradation at the application site due to proteases, moreover; when wounds are painful the patient fails to apply any remedy frequently. In the present study, insulin has been formulated as a spray in liposomes, which protects it at the wound area and sustains its release and thus reducing the application frequency, furthermore; the spray reducing the direct contact of the applicator with the skin, thus, reducing the probability of infection. The full-factorial design has been applied in the preparation optimization of liposomes, where the effects of the cholesterol, method of preparation and sonication have been tested on the particle size and the entrapment efficiency. The present study shows how the thin film hydration method in the absence of cholesterol and sonication were the best conditions for insulin. liposomal formulation, that satisfies the target of the study. Liposomes showed a sustained release of insulin up to 24 hours and were successfully formulated into a spray dosage form. In conclusion, topical insulin liposomal spray offers a protective method from insulin degradation with an expected increase in the patient compliance. � 2018 Marwa H. S. Dawoud et al.