Browsing by Author "Gamal Abd EL-Nasser, Omar"

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    Advantages of Mesenchymal stem cells (MSCs) in Regenerative Medicine
    (October University for Modern Sciences and Arts, 2018) Mohamed Anis, Abdul Rahman; Mohsen Ahmad, Mai; Gamal Abd EL-Nasser, Omar
    This study aimed at Evaluation of effect of Different surface modifications of 3D printed PCL scaffolds on the immunogenicity of mesenchymal stem cells Aim: The aim of this study was the isolation and expansion of mesenchymal stem cells )MSCs (from dental pulp .and testing the effect of different surface modifications of polycaprolactone (PCL) membrane on the immunogenicity of MSCs. Methods: This In vitro study aimed at first for the isolation of MSCs from dental pulp tissue by enzyme digestion method and expanding them in culture. Second, studying of the immune phenotype of stem cells by flow cytometric analysis when exposed to surface modified scaffolds used for tissue regeneration. Data was reported as mean ± standard error. Statistical analysis was performed using Microsoft (MS) Office Excel Software. One-way ANOVA was used to asses for differences between groups, and p values were calculated by Student’s t-test. Differences were considered statistically significant if the p-value was less than 0.05 Data was reported as mean ± standard error.