Browsing by Author "Fouad, Manal M"

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Application of NEMI, Analytical Eco-Scale and GAPI tools for greenness assessment of three developed chromatographic methods for quantification of sulfadiazine and trimethoprim in bovine meat and chicken muscles: Comparison to greenness profile of reported HPLC methods
(Elsevier Inc., 3/26/2020) Mohamed, Dalia; Fouad, Manal M
Employing green analytical chemistry approaches to newly developed methods has extremely gained the interest of the analytical community and was intensely established in the past few years. The utilized solvents as well as the amount of produced waste have the chief impact on evaluating the greenness of the method. The present work is devoted to the development and the assessment of the greenness profile of three developed chromatographic methods for the quantitative estimation of sulfadiazine (SDZ) and trimethoprim (TMP) in bovine meat and chicken muscles using three evaluation tools; the “National Environmental Method Index” (NEMI), the “Analytical Eco-Scale” and the “Green Analytical Procedure Index” (GAPI). The developed methods have employed a variety of mobile and stationary phases and offered different quantification ranges. Conventional RP-HPLC with green aqueous-organic mobile phase was applied in Method I with the composition of water: ethanol (90: 10 v/v, pH = 5.5) utilizing a Gemini C18 column (150 × 4.6 mm, 5 µm) with linear ranges of 1–60 μg/mL for SDZ and 1–50 μg/mL for TMP. Micellar liquid chromatography (MLC) as an efficient green alternate was used in Method II with mobile phase composition of 0.1 M SDS, 12% isopropanol in 0.1 N citric acid buffered at pH 5.5 while utilizing an XTerra RP18 column (150 × 3.9 mm, 5 µm) and resulting in a linear range of 0.5–100 μg/mL for both drugs. UPLC-MS/MS as a high throughput analytical technique was utilized in Method III with a mobile phase composed of ammonium acetate: acetonitrile (80: 20 v/v, pH = 5.5) and an Acquity UPLC-BEH C18 column (50 × 2.1 mm, 1.7 μm), where the linear range was 0.1–2 μg/mL for both drugs. Thus, Methods II and III have permitted the estimation of the residues of SDZ and TMP at levels equivalent to their maximum residue limits. The three assessment tools have agreed that Methods II and III are the greenest developed methods, thus, recommending the utilization of MLC and UPLC-MS/MS as green, safe practice for estimating the drugs’ residues in food of animal origin.
Densitometric and UV-Spectrophotometric Methods for Simultaneous Determination of Spiramycin adipate in Binary Mixture with Oxytetracycline-HCl or Tetracycline-HCl
(NLM, 2018) El Sanabary, Hoda F; Fouad, Manal M; Abdel Razeq, Sawsan A.; El Demerdash, Asmaa O.
Five methods were developed for simultaneous determination of spiramycin adipate and oxytetracycline-HCl and/or tetracycline-HCl in their pharmaceutical formulations. The first one was a densitometric evaluation of thin-layer chromatograms using a mobile phase of methanol: Butanol: Chloroform: Ammonia 1% (5:1:1:1, by volume). The plates were visualized under UV lamp at 254 nm where spots appeared at Rf 0.76, 0.30 and 0.37 for spiramycin adipate, oxytetracycline-HCl and tetracycline-HCl, respectively. The chromatograms of the drugs were measured densitometrically at 240 nm for spiramycin adipate and at 350 nm for both oxytetracycline-HCl and tetracycline-HCl in the range of 0.1-0.8 µg mL-1 and 0.1-1.0 µg mL-1, respectively. Likewise, the simultaneous estimation of the cited drugs was performed by four spectrophotometric methods. Method A was a mean centering (MC) which provided spiramycin adipate determination at 232 nm t oxytetracycline-HCl or tetracyclinea third derivative (3D) through which spiramycin adipate could be estimated at 256 nm while the wavelength 281 nm was selected for oxytetracyclin C was a derivative ratio (1DR) that was established to determine spiramycin adipate only in the presence of oxytetracycline-HCl or tetracycline dual wavelength (IDW) which allowed the determination of oxytetracycline spiramycin adipate and up to 50% of its impurity tetracycline. the concentration range of 5-70 oxytetracycline-HCl and tetracycline to be in accordance with those given by reported methods. The validity of the methods was evaluated according to ICH guidelines.
Determination of Menbutone in Bovine Milk and Meat Using Micellar Liquid Chromatography: Application to Injectable Dosage Forms (vol 9, pg 638, 2016)
(Springer, 2016) Belal, F; Abd El-Razeq, Sawsan A.; Fouad, Manal M; Zayed, S; Fouad, Fatma A
The original version of this article unfortunately contained a mistake. Affiliation 4 should be included for Manal M. Fouad.
Development and Validation of Green Spectrophotometric Methods for Simultaneous Determination of Paracetamol, Pamabrom and Pyrilamine Maleate in Bulk and Pharmaceutical Dosage Form
(SCIENCEDOMAIN INT, 2017) Mahmoud, Ebtesam S; Rashed, Noha S; Fouad, Manal M; Habib, Fawzia I
Four simple and accurate spectrophotometric methods were developed for the simultaneous determination of paracetamol, pamabrom and pyrilamine maleate. The first is a zero order spectrophotometric method used for the determination of pyrilamine maleate in presence of paracetamol and pamabrom in the range of 0.5-60 mu g/mL by measuring the absorbance at 306.8 nm where paracetamol and pamabrom exhibits zero reading. The other three methods; bivariate, dual wavelength and area under the curve methods were developed for the simultaneous determination of paracetamol, pamabrom in presence of pyrilamine maleate. Bivariate calibration algorithm involves the use of two selected wavelengths; 260 nm and 280 nm for the determination of the two studied drugs. For the dual wavelength, paracetamol shows equal absorbance at 212.87 and 220.0 nm, where the differences in absorbance were measured for the determination of PBM. Similarly, differences in absorbance at 264.23 nm and 292.28 nm were measured for determination of paracetamol. In the area under curve method; the area between 237.14 to 247.14 nm was used for determination of paracetamol and 273.6 to 283.6 nm for pamabrom. Beer's law was obeyed in the concentration ranges of 1-30 mu g mL(-1) for paracetamol and pamabrom in all methods. LOD was calculated and ranges from 0.149-0.766 mu g/mL, while LOQ was found to be in the range of 0.556-1.145 mu g/mL. The proposed methods were successfully applied for the simultaneous determination of PCM, PBM and PAM in their pharmaceutical preparation without interference from additives present with mean recovery ranging from 98.99 to 101.44%. Statistical analysis of the results obtained by the proposed spectrophotometric methods compared with a reported method revealed no significant difference between the proposed and reported methods confirming accuracy and precision at 95% confidence limit. Accession Number: WOS:000428425300003
Green Prospective Approach of Chromium Zinc Oxide Nanoparticles for Highly Ultrasensitive Electrochemical Detection of Anti-hypotensive Medication in Various Matrices
(American Chemical Society, 2023-08) Otaif, Khadejah D; Fouad, Manal M; Rashed, Noha S; Hosni, Noha Y.Z; Elsonbaty, Ahmed; Elgazzar, Elsayed
A highly ultrasensitive sensor that relied on Cr/ZnO-NPs was developed to detect etilefrine hydrochloride (ETF) in different matrices via a particular green voltammetric technique. The X-ray diffraction pattern showed the nanomaterials of the polycrystalline hexagonal structure. The energy-dispersive X-ray spectrum approved the presence of Cr3+ inside the host zinc oxide framework. The morphological and topological characteristics were visualized using transmission electron microscopy and atomic force microscopy micrographs describing the nanoparticles in spherical-like shape with large-surface area. The energy gap (Eg) was evaluated from transmittance (T %) and reflectance (R %) spectra within the visible region. The optimization study indicated that the Cr/ZnO-NP/CPE sensor has high sensitivity, thanks to the distinctive physical and chemical properties of the fabricated electrode. A new approach showed a great selectivity for determining ETF in different matrices in the presence of other interferents like levodopa. Under optimal circumstances, the square- wave voltammetry revealed a linear response to ETF from 0.01 to 10 μmol L−1 (r = 0.9996) with quantification and detection limits of 9.11 and 2.97 nmol L−1 , respectively. Finally, the proposed approach was effectively applied to estimate ETF in pharmaceutical dosage forms and biological fluids using simple, accurate, and selective electrochemical electrode. The greenness profile assessment of the developed method was performed using the Eco-Scale and green analytical procedure index. These tools indicated that the proposed method is an eco-friendly technique for the determination of ETF in different matrices.