Browsing by Author "Fayez, Ahmed M."

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1 of 1 Eicosapentaenoic Acid and Vitamin E Against Doxorubicin-Induced Cardiac and Renal Damages: Role of Cytochrome c and iNO
(ACAD MEDICAL SCIENCES I R IRAN, 2018) Fayez, Ahmed M.; Zaafan, Mai A.
Background: The current study aimed to evaluate the mechanisms involved in protection against doxorubicin-induced cardiac and renal toxicities upon treatment with eicosapentaenoic acid and vitamin E. Methods: Rats were randomly assigned to 4 groups: normal control, doxorubicin inducted control, eicosapentaenoic acid treated group and a final group pretreated with vitamin E. Lipid peroxidation, reduced glutathione (GSH) and tumor necrosis factor-alpha (TNF-alpha) contents as well as glutathione peroxidase (GPx), superoxide dismutase (SOD) and myeloperoxidase (MPO) activities were assessed. Moreover, hearts were used for immunohistochemical detection of the pro-apoptotic protein cytochrome c expression, while the kidneys were used for detection of inducible nitric oxide synthase (iNOS) expression. Results: Eicosapentaenoic acid and vitamin E produced significant protection from doxorubicin-induced cardiac and renal toxicities. The suggested mechanisms for protection included decreased cytochrome c and iNOS expression as well as markedly decreased lipid peroxides and TNF-alpha contents accompanied with increased GSH content as compared to the doxorubicin control group. Moreover, there was marked increase in GPx and SOD activities accompanied by significant suppression of MPO activity. Conclusion: The present study demonstrated the potent protective effects of eicosapentaenoic acid and vitamin E from doxorubicin induced cardiac and renal toxicities through their potent anti-oxidant, anti-inflammatory and anti-apoptotic properties. Hence, eicosapentaenoic acid and vitamin E could be promising protective agents against doxorubicintoxicity.
Amelioration of Autoimmunity and Inflammation by Zinc Oxide Nanoparticles in Experimental Rheumatoid Arthritis
(ORCID, 2021-01) Shaaban, Sameh; Fayez, Ahmed M.; Abdelaziz, Mahmoud; Abou El-ezz, Doaa
Rheumatoid arthritis (RA) is a chronic autoimmune disease that affects the lining of the synovial joints and approximately affects 0.5-1% of the total population imposing a socioeconomic burden. Currently, there is no cure for RA, but receiving proper medical care at early stages of the disease is of high importance, to prevent the progressive disability and premature death. Using rat animal model injected with Complete Freund’s adjuvant proved to be successful in induction of a state highly resembling RA in human. Zinc oxide nanoparticles (ZnO NPs) are considered as one of the most important metal oxide nanoparticles due to their exclusive properties, and they are currently merged in several biological applications due to their biocompatibility, low cost, and high safety prole. In this study, we demonstrated the novel possible benecial effects of using zinc oxide nanoparticles, on such devastating severe disease. Zinc oxide nanoparticles (ZnO NPs) proved to reduce the adjuvant-induced increased productions of IL-1β, TNF-α, IL-10, total leukocyte count, rheumatoid factor, anti-CCP levels in rats, suggesting an interesting option to be available either alone or in combinations to better control RA. In conclusion we recommend the expansion of more in vivo studies to highlight the benets which could be obtained of nanoparticles either alone or in combination with the known anti-arthritic and/or antiinammatory agents; giving rise to new protocols to maximize the control of RA
Reduction of intraocular pressure using timolol orally dissolving strips in the treatment of induced primary open-angle glaucoma in rabbits
(royal pharmaceutical society, 2020-03) El‐Feky, Yasmin A.; Mostafa, Dalia A.; Al-Sawahli, Majid M.; El‐Telbany, Rania Farag A.; Zakaria, Sherin; Fayez, Ahmed M.; Ahmed, Kawkab A.; Ebtesam M Alolayan; Dalia Farag A El-Telbany
Objective To enhance bioavailability of timolol (TML) and utilize alternatives for traditional eye drops for more patient compliance, this study was aiming to develop biodegradable orally dissolving strips (ODSs) of TML for treatment of primary open-angle glaucoma (POAG). Methods Novel ODSs of TML were formulated and optimized using solvent casting method according to full factorial design (31 .22 ). TML ODSs were charac- terized with respect to many parameters. In-vivo test was carried out using four groups of 24 New Zealand albino rabbits. POAG was induced by subconjunctival treatment of betamethasone. Histopathological examination and oxidative stress markers assay were carried out. Key findings The optimized formula (F9) exhibited a remarkably 15-s disinte- gration time and 96% dissolution rate after 10 min. The results revealed a potent significant inhibitory effect of the optimized TML ODS to reduce IOP in induced rabbits in comparison with control rabbits and TML eye drops-treated rabbits. The formula showed also high activity against oxidative stress and absence of histopathological changes in iridocorneal angle and cornea. Conclusion The ODSs could be a promising alternative delivery system for eye drops with more compliance to enhance delivery and therapeutic activity of TML in treatment of POAG.