Browsing by Author "Fayed M.A.A."

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Centaurea pumilio L. extract and nanoparticles: A candidate for healthy skin
(Elsevier B.V., 2019) Mostafa E.; Fayed M.A.A.; Radwan R.A.; Bakr R.O.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; University of Sadat City; Egypt; Biochemistry and Biotechnology Department; Faculty of Pharmacy & Drug Technology; Heliopolis University; Cairo; Egypt
Centaurea pumilio was the subject of phytochemical and biological studies, and its extract was used in the green synthesis of silver nanoparticles (AgNPs). Liquid chromatography/electrospray ionization mass spectrometry allowed the tentative identification of twenty-nine phytoconstituents of C. pumilio methanolic extract (CME), while column chromatography led to the identification of eight phenolic compounds. The neutral red uptake method showed the safety of CME and AgNPs on skin cells (HaCaT cell lines), while their high antioxidant potentials were demonstrated based on their oxygen radical absorbance capacity, and these results were confirmed in vivo. Additionally, CME and AgNPs had promising abilities to retard the ageing process and combat dark spots by potently inhibiting collagenase, elastase and tyrosinase, in addition to antimicrobial activity against skin infection-causing strains, especially Staphylococcus aureus, which was further confirmed by the significant phagocytic activity of neutrophils via engulfment. This study presents C. pumilio as a candidate for healthy skin. 2019 Elsevier B.V.
A completely polyherbal conditioning and antioxidant shampoo: A phytochemical study and pharmaceutical evaluation
(International Journal of Pharmacognosy and Phytochemical Research, 2019) Bakr R.O.; Amer R.I.; Fayed M.A.A.; Ragab T.I.M.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; 11787; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; University of Sadat City; Sadat City; Egypt; Department of Chemistry of Natural and Microbial Products; Pharmaceutical Industries Research Division; National Research Centre; Giza; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt
Aim: A recent approach in shampoo research has been to find a natural alternative for synthetic detergents that have deteriorative effects on hair follicles. This study aimed at the formulation of a completely herbal shampoo containing a natural foaming agent, in addition to having conditioning, antioxidant, and antimicrobial effects. Material and Methods: The leaves of Salix babylonica L., Ziziphus spina-christi L. (Willd), and Glycyrrhiza glabra rhizomes were extracted with 70% methanol then quantified for their phenolic and flavonoid contents using colorimetric assays that were qualitatively identified by Ultra-performance liquid chromatography (UPLC) with tandem mass spectrometry (MS-MS). The mineral content was also estimated. The radical scavenging activity was estimated using 1,1-diphenyl-2-Picrylhydrazyl (DPPH) and the half maximal inhibitory concentration (IC50) was determined. Additionally, the antimicrobial activity was tested using agar diffusion assay and compared to vancomycin and ketoconazole. Four formulations, consisting of the different plant extracts and a combination of the extracts, were prepared and evaluated for several physicochemical properties. The best formula was evaluated for its conditioning effects using scanning electron microscope and blind touch tests by asking volunteers for grading the formulations. Results and Discussion: UPLC-MS-MS analysis of S. babylonica and Z. spina-christi allowed tentative identification of 12 phytoconstituents in each. Z. spina-christi showed the highest phenolic content and a high copper, zinc, and manganese content beside the best antioxidant activity, whereas G. glabra had a high potency against Bacillus cereus and Candida albicans. The polyherbal shampoo formulation (F4) was selected as an optimized formulation because of a high foam stability after 4 min, low wetting time (2 s), surface tension reduction, and comparable results for percent solid content. F4 showed good conditioning effect and consumer contentment. Conclusion: The formulated polyherbal shampoo is chemical free, extra-nourishing shampoo with excellent conditioning, cleansing, and antimicrobial effects. � 2019 Journal of Pharmacy and Bioallied Sciences.
Hepatoprotective activity of Erythrina neillii leaf extract and characterization of its phytoconstituents
(Elsevier GmbH, 2019) Bakr R.O.; Fayed M.A.A.; Fayez A.M.; Gabr S.K.; El-Fishawy A.M.; Taha S.El-Alfy; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; El-Sadat City University; Egypt; Department of Pharmacology; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt
Background: Natural antioxidants and anti-inflammatory agents have the ability to restore normal balance to destructed liver cells. The genus Erythrina has attracted attention for its broad spectrum of physiological activities and its rich polyphenolic and alkaloid contents. Hypothesis/Purpose: The major phytoconstituents of Erythrina neillii, an ornamental coral tree and a hybrid between E. herbacea and E. humeana that was not previously studied, were investigated. The hepatoprotective effect and underlying mechanisms were also assessed. Study design and methods: The main phytoconstituents in the different fractions of the alcoholic leaf extract (dichloromethane and ethyl acetate) were identified using high resolution high-performance liquid chromatography coupled with mass spectrometry (HR-HPLC-MS-MS) based on the fragmentation pattern and molecular formula of the identified compounds and on previous literature. In addition, the hepatoprotective, anti-inflammatory and antioxidant activities of three doses of E. neillii alcoholic leaf extract (100, 250, 500 mg/kg) were investigated in methotrexate (MTX)-intoxicated rats and were compared with those of silymarin-treated rats. Liver function parameters were obtained, and a histopathological study was performed. In addition, the anti-inflammatory mediators and the antioxidant system in the liver tissues were assessed. Results: The dichloromethane extract revealed an abundance of alkaloids (25), in addition to tentatively identifying flavone (1), flavanone (1) and three fatty acids. Additionally, thirty-six compounds belonging to different classes of phytoconstituents with a predominance of flavonoids (21), O/C-flavone and flavonol glycosides, followed by alkaloids (9), fatty acids (4) and (2), and phenolic glycoside were identified in the ethyl acetate extract. Compared with MTX, alcoholic leaf extract (500 mg/kg) ameliorated the MTX-induced alterations by improving several biochemical marker levels, fighting oxidative stress in serum and liver tissues, and decreasing inflammatory mediators; this finding was further confirmed by the histopathological study. Conclusion: This study reveals E. neillii, a rich source of flavonoids and alkaloids, which could be further exploited to provide a promising and safe antihepatotoxic agent source. 2018 Elsevier GmbH