Browsing by Author "F. Rahman, M."

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    CONTRIBUTION OF NAD (P) H OXIDASE P22 PHOX AND ENOS GENE POLYMORPHISM IN THE PREDISPOSITION OF EARLY ONSET ACUTE MYOCARDIAL INFARCTION IN EGYPTIAN POPULATION
    (WALTER DE GRUYTER & CO., 2011) M. Hashad, I.; F. Rahman, M.; M. Shaban, G.; Z. Gad, M.
    Background. C242T polymorphism of the p22 phox gene, an essential component of NAD(P)H oxidase in vasculature, and Glu298Asp polymorphism of endothelial nitric oxide synthase (eNOS) gene have been recently implicated as genetic markers for acute myocardial infarction (AMI) in many studies. The aim of this study is to collect information about the prevalence of these two polymorphisms and their relationship with occurrence of early onset AMI in Egyptian populations. Methods. The study subjects consisted of 104 AMI patients and 101 aged matched volunteers. Genotyping of both genes was done by the polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. Results. The genotype distribution for NAD(P)H oxidase p22 phox gene was significantly different between AMI patients (CC;41.3%, CT;58.7%, TT;0%) and control subjects (CC;27%, CT;72%, TT;1%) (P=0.026), whereas eNOS gene distribution was not significantly different between AMI patients (GG;49%, GT;45.2%, TT;5.8%) and control subjects ( GG;58.4%, GT;33.6%, TT;7.9%) (P= 0.367). Conclusions. The prevalence of the CT+TT genotype of the C242T polymorphism was significantly more frequent in control subjects than in AMI patients. Our observations suggest that C242T polymorphism of the p22 phox gene of NAD(P)H oxidase may reduce susceptibility to AMI and is a novel genetic marker that has a protective effect on coronary risk while no significant association was observed between the Glu298Asp polymorphism of eNOS gene and incidence of AMI in this Egyptian population group
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    ENOS (G894T) GENE POLYMORPHISM IN A RANDOM SAMPLE OF EGYPTIAN POPULATION: LACK OF CORRELATION TO SERUM NO LEVELS
    (WALTER DE GRUYTER & CO., 2011) F. Rahman, M.; M. Abdel-Maksoud, S.; Abou-Aisha, K.; Z. Gad, M.
    Background. eNOS gene has been extensively screened for variation. The only common variation identified is the G894T (guanine/thymine substitution) at exon 7 that leads to glutamate/aspartate substitution at position 298 in the mature protein. This study aimed to detect eNOS gene polymorphisms in Egyptian population to be used as a reference for future studies, and to explore functional correlations of eNOS genotypes with serum nitric oxide (NO). Methods. Random unrelated 101 healthy subjects were recruited for the study from the volunteers attending the blood bank at 57357 Hospital in Cairo, Egypt. eNOS genotypes were determined by PCR-RFLP. Serum NO was determined spectrophotometrically. Results. Genotype distribution of eNOS Glu298Asp polymorphism in Egyptian population was 58.42% GG (wild type), 33.66% GT and 7.92% TT genotypes while the allele frequencies were 75.25% and 24.75% for the G and T alleles, respectively. No significant association between serum NO and specific eNOS genotype. Conclusions. The present study demonstrated the predominance of the homozygous genotype GG over the heterozygous GT and homozygous TT in random samples of Egyptian population. It also showed the lack of association between eNOS genotypes and mean serum levels of NO.