Browsing by Author "F. MAKLAD1, M."

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    MOLECULAR MARKERS FOR SELF INCOMPATIBILITY PHENOMENA IN SOME MANGO CULTIVARS
    (Egypt. J. Genet. Cytol.,, 2012) F. MAKLAD1, M.; DESOUKY, M.
    Mango (Mangifera indica L.) is considered one of the oldest cultivated trees in the world. Low productivity of some mango cultivars is associate with low fruit setting and/or high fruit drop of immature fruits. The self and cross incompatibility has been reported as one of the serious factor affecting fruit set in many mango cultivars. In this respect, five elite mango cultivars (Alphonse, Ewais, Hindi khassa, Keitt and Zebda) were examined for their self and cross- incompatibility. RAPD analysis was performed to assess the genetic variation in cross-incompatibility between them. The cross-compatibility was estimated by counting the number of setted fruits per panicles at 21 days after cross pollination between every two cultivars. At the same time, the fluorescence microscope was used to determine the growth of pollen tube in the style tissue after pollination. The obtained results showed that, the five tested mango cultivars in this study were highly similar at the DNA level. Ewais, Hindi khassa and Alphonse can be grouped together showing high similarity between them. The highest similarity value was observed between Hindi khassa and Ewais cultivars, while the lowest similarity was recorded between Ewais and Zebda cultivars. Hindi Khassa and Ewais which appear to be the most similar cultivars showed the lowest percentage of pistils with pollen tubes reaching the base