Browsing by Author "Ezzat, Shahira"

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Evaluation of antioxidant activity in diferent Egyptian barley cultivars: an in vitro and in silico study
(Springer open, 2024-05) Eid, Omneya; Ezzat, Shahira; Elkady, Wafaa M; El Sayed, Abeer; Abd el‑sattar, Essam
Background Cereals have historically played a crucial role in the human diet, serving as a signifcant natural source of energy and ofering various health benefts. Barley (Hordeum vulgare L.) has been given signifcant attention in recent years due to its exceptional nutritional value, surpassing that of other cereals. The objective of this research is to evaluate the antioxidant activity of various solvent extracts obtained from three diferent barley cultivars. Results The G.136 variety’s acetone extract exhibited the highest level of antioxidant activity in both the DPPH assay, with an IC50 of 55.62 µg/ml, and the FRAP assay, with 447 μM trolox/mg extract. The dominant compounds identi‑ fed before in the acetone fraction were subjected to an evaluation of their docking scores, along with an assessment of ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) and TOPKAT (Toxicity Prediction by Komputer Assisted Technology) studies. Notably, hordatine A1, prodelphinidin B3, hordatine B1, procyanidin B2, and isovitexin 7-O-glucoside were the major compounds with the highest LipDock scores compared to trolox the reference drug with polyphenol oxidase. Conclusions The fndings indicate that the acetone extract from all three cultivars demonstrates noteworthy results, surpassing the efcacy of other solvent extracts against the antioxidant activity.
Metabolomic profiling of barley extracts obtained via different solvents and evaluation of their anti-inflammatory efficacy
(Elsevier B.V, 2024-07) Eid, Omneya; Salem, Mohamed A; Mohamed, Osama G; Ezzat, Shahira; Tripathi, Ashootosh; Ewida, Menna A; El Sayed, Abeer; Abdel-Sattar, Essam; Elkady, Wafaa M
Barley (Hordeum vulgare L.) stands out for its special nutritional value compared to other cereals, garnering significant attention in recent years. In this study, a comprehensive chemical and biological evaluation of different barley cultivars extracted with different solvents was performed. Ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QTOF-MS/MS) was used in the phytochemical study. The molecular docking with absorption, distribution, metabolism, excretion, and toxicity (ADMET) study and multivariate analysis alongside the quantification of total phenolic contents (TPC) were performed. Furthermore, the anti-inflammatory action was in vitro assessed by measuring COX-1, COX-2, and 5-LOX inhibition. This study seeks to establish a suitable solvent for extracting anti-inflammatory properties from diverse barley cultivars. The methanol extract obtained from the G.131 cultivar, followed by the 80% methanol extract from the G.136 cultivar, exhibited the most favorable IC50 values in comparison to the reference drugs indomethacin and celecoxib. The phytochemical profile revealed more than 60 compounds that belonged to nitrogen-containing compounds, phenolic compounds, organic acids, flavonoids, and their glycosides, hordatines, and other classes. The TPC and COX-2 inhibition was positively correlated with isoscoparin, coumaroyle–OH–eagmatine, isoorientin, isovitexin 7-O-rhamnosylglucoside, isoscoparin 7-O-glucoside, and isoorientin-7-O-[6-feruloyl]-glucoside. These findings along with their pharmacodynamics study suggested a potential synergistic and significant contribution of these compounds with the anti-inflammatory activity.
The potential of Genus Rumex as a valuable source of health-promoting metabolites: A review of ethnomedicinal and pharmacological uses in the treatment of skin diseases
(NIDOC (Nat.Inform.Document.Centre), 2024-08) Ezzat, Shahira; El Hawary, Seham; kirollos, farid; Gohar, Mai
Skin diseases display essential disorders that threaten human health worldwide thus, there is an urge to find effective remedies for treating such diseases, especially from natural products which can be safer, more available, and cheaper than synthetic drugs. Rumex is one of the interesting drugs known to have a beneficial curing effect on various skin disorders especially through exerting antiaging and depigmentation activities. Genus Rumex is the second largest genus in the Polygonaceae family which consists of almost 200 species that are rich in various bioactive compounds such as flavonoids, anthraquinones, chromones, and stilbenes. The presence of numerous classes of bioactive constituents in Rumex was reflected in its pharmacological activities, thus the plant has potential anti-cancer, anti-diabetic, anti-inflammatory, and anti-oxidant effects together with its antiaging, anti-wrinkes and skin depigmentation. In this review, we provided an overview of the active compounds of Genus Rumex as well as its biological activity with a special emphasis on its effect on skin diseases.