Browsing by Author "Essa, BM"

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    99mTc-amitrole as a novel selective imaging probe for solid tumor: In silico and preclinical pharmacological study
    (Elsevier, 2015) Essa, BM; Sakr, TM; A Khedr, Mohammed; El-Essawy, FA; El-Mohty, AA
    Lactoperoxidase (LPO) inhibitors are very selective for solid tumor due to their high binding affinity to the LPO enzyme. A computational study was used to select top-ranked LPO inhibitor (alone and in complex with 99mTc) with high in silico affinity. The novel prepared 99mTc-amitrole complex demonstrated both in silico and in vivo high affinity toward solid tumors. 99mTc-amitrole was radio-synthesized with a high radiochemical yield (89.7 ± 3.25). It showed in vitro stability for up to 6 h. Its preclinical evaluation in solid tumor-bearing mice showed high retention and biological accumulation in solid tumor cells with a high Target/Non-Target (T/NT) ratio equal to 4.9 at 60 min post-injection. The data described previously could recommend 99mTc-amitrole as potential targeting scintigraphic probe for solid tumor imaging
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    Synthesis and biodistribution of 99mTc-PyDA as a potential marker for tumor hypoxia imaging
    (SPRINGER, 2014) Sakr, TM; Essa, BM; El-Essawy, FA; El-Mohty, AA
    The 99mTc-pyrimidine-4,5-diamine (99mTc-PyDA) complex was prepared. The yield under the optimum conditions (5 mg of PyDA, pH 8, 25 μg of SnCl2·2H2O) was 96 ± 3%. The complex is stable in vitro for 24 h. The complex was tested on mice as potential marker for tumor hypoxia imaging. The complex showed high tumor hypoxia uptake with the target/nontarget (T/NT) ratio as high as ∼3.