Browsing by Author "Elgizawy S.M."

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Comparative study of new spectrophotometric methods; An application on pharmaceutical binary mixture of ciprofloxacin hydrochloride and hydrocortisone
(Sociedad Chilena de Quimica, 2013) Lotfy H.M.; Hassan N.; Elgizawy S.M.; Saleh S.S.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini Street; 11562 Cairo; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Assuit University; 71515 Assuit; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Egypt
Simple, specific, accurate and precise spectrophotometric methods were developed and validated for simultaneous estimation of Ciprofloxacin hydrochloride (CIP) and Hydrocortisone (HYD) in their pure form and pharmaceutical dosage form. Two new spectrophotometric methods were applied: extended ratio subtraction (EXRSM) and ratio difference (RDSM) methods. The results were compared to three well-established methods: Mean centering of ratio spectra method (MCR), Isoabsorpative point spectrophotometric method (IsoM) and Absorbance ratio method (ARM). The linearity range for the spectrophotometric methods was found to be (2-14 ?g/mL) and (1-14 ?g/mL) for CIP and HYD respectively. The selectivity of the developed methods was investigated by analyzing laboratory prepared mixtures of the two drugs and their combined dosage form. The results obtained from the proposed methods were statistically compared using one-way analysis of variance (ANOVA) where no significant difference was observed between the new (EXRSM and RDSM) and the well-established methods which prove their validity for the analysis of this binary mixture. The methods were validated as per ICH guidelines regarding accuracy, precision, repeatability and robustness; which were found to be within the acceptable limits. The new methods were simple, sensitive and don't need a special program, so they could be easily applied as alternative methods to LC methods in quality control laboratories lacking the required facilities for those techniques.
A comparative study of validated spectrophotometric and TLC- spectrodensitometric methods for the determination of sodium cromoglicate and fluorometholone in ophthalmic solution
(2013) Saleh S.S.; Lotfy H.M.; Hassan N.Y.; Elgizawy S.M.; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 11787 6th October City; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr-El Aini Street; 11562 Cairo; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; Assuit University; 71515 Assuit; Egypt
The determination of sodium cromoglicate (SCG) and fluorometholone (FLU) in ophthalmic solution was developed by simple, sensitive and precise methods. Three spectrophotometric methods were applied: absorptivity factor (a-Factor method), absorption factor (AFM) and mean centering of ratio spectra (MCR). The linearity ranges of SCG were found to be (2.5-35?g/mL) for (a-Factor method) and (MCR); while for (AFM), it was found to be (7.5-50?g/mL). The linearity ranges of FLU were found to be (4-16?g/mL) for (a-Factor method) and (AFM); while for (MCR), it was found to be (2-16?g/mL). The mean percentage recoveries/RSD for SCG were found to be 100.31/0.90, 100.23/0.57 and 100.43/1.21; while for FLU, they were found to be 100.11/0.56, 99.97/0.35 and 99.94/0.88 using (a-Factor method), (AFM) and (MCR), respectively. A TLC-spectrodensitometric method was developed by separation of SCG and FLU on silica gel 60 F254 using chloroform:methanol:toluene:triethylamine in the ratio of (5:2:4:1v/v/v/v) as developing system, followed by spectrodensitometric measurement of the bands at 241nm. The linearity ranges and the mean percentage recoveries/RSD were found to be (0.4-4.4?g/band), 100.24/1.44 and (0.2-1.6?g/band), 99.95/1.50 for SCG and FLU, respectively. A comparative study was conducted between the proposed methods to discuss the advantage of each method. The suggested methods were validated in compliance with the ICH guidelines and were successfully applied for the determination of SCG and FLU in their laboratory prepared mixtures and commercial ophthalmic solution in the presence of benzalkonium chloride as a preservative. These methods could be an alternative to different HPLC techniques in quality control laboratories lacking the required facilities for those expensive techniques. 2012.