Browsing by Author "Elgizawy, Samia M."

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    A Comparative Study of the Novel Ratio Difference Method versus Conventional Spectrophotometric Techniques for the Analysis of Binary Mixture with Overlapped Spectra
    (Scientific Research Publishing, 2012) Elgizawy, Samia M.; Hassan, Nagiba Y; Saleh, Sarah S; Lotfy, Hayam M
    The smart novel ratio difference spectrophotometric method was developed and validated for the determination of a binary mixture of Sodium cromoglicate (SCG) and Fluorometholone (FLU) in presence of benzalkonium chloride without prior separation. The results were compared to that of the conventional methods (dual wavelength and first derivative of ratio spectra). The suggested methods were validated in compliance with the ICH guidelines and were successfully applied for determination of SCG and FLU in their laboratory prepared mixtures and commercial ophthalmic solution. The novel method showed significant advantages over the conventional methods regarding simplicity, minimal data manipulation and maximum reproducibility and robustness; which enabled the analysis of binary mixtures with overlapped spectra for routine quality control testing with quite satisfactory.
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    Univariate versus Multivariate Spectrophotometric Methods for Simultaneous Determination of Complex Binary Mixtures with Overlapped Spectra: A Comparative Study
    (Taylor & Francis, 2013-09) Elgizawy, Samia M.; Hassan, Nagiba Y; Saleh, Sarah S.; Lotfy, Hayam M
    Two novel simple and accurate univariate spectrophotometric methods were described for the determination of a binary mixture of Sodium cromoglicate (SCG) and Fluorometholone (FLU) namely; ratio subtraction coupled with extended ratio subtraction (RS-EXRSM) and constant center (CCSM) spectrophotometric methods. The methods were able to recover the zero order absorption spectra of both drugs from their binary mixture through simple mathematical calculations. The linearity ranges were found to be (2.5–25 µg/mL) and (4- 25 µg/mL) for SCG and FLU, respectively. A comparative study was conducted between the proposed univariate methods versus multivariate ones, based on using principle component regression (PCR) and partial least squares (PLS-2). Upon applying the methods for the determination of SCG and FLU in laboratory prepared mixtures, the (CCSM and PLS-2) proved to be of higher predictive ability rather than (RS-EXRSM and PCR) in prediction of mixtures containing low concentration of one of the components. The proposed methods were validated in compliance with the ICH guidelines. These methods could be alternative to different HPLC techniques in quality control laboratories lacking the required facilities for those expensive techniques