Browsing by Author "El-Naggar A.M."

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    Synthesis, characterization and molecular docking studies of thiouracil derivatives as potent thymidylate synthase inhibitors and potential anticancer agents
    (Springer International Publishing, 2017) El-Naggar A.M.; Abou-El-Regal M.M.; El-Metwally S.A.; Sherbiny F.F.; Eissa I.H.; Chemistry Department; Faculty of Science; Ain Shams University; Abbassia; Cairo; 11566; Egypt; Higher Technology Institute; 10th of Ramadan City; Egypt; Organic Chemistry Department; Faculty of Pharmacy (Boys); Al-Azhar University; Cairo; 11884; Egypt; Organic Chemistry Department; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); 6th October City; 11787; Egypt; Pharmaceutical Chemistry Department; Faculty of Pharmacy (Boys); Al-Azhar University; Cairo; 11884; Egypt
    Thymidylate synthase (TS), one of folate-dependent enzymes, is a key and well-recognized target for anticancer agents. In this study, a series of 6-aryl-5-cyano thiouracil derivatives were designed and synthesized in accordance with essential pharmacophoric features of known TS inhibitors. Nineteen compounds were screened in vitro for their anti-proliferative activities toward HePG-2, MCF-7, HCT-116, and PC-3 cell lines. Compounds 21c, 21d, and 24 exhibited high anti-proliferative activity, comparable to that of 5-fluorouracil. Additionally, ten compounds with potent anti-proliferative activities were further evaluated for their ability to inhibit TS enzyme. Six compounds (21b, 21c, 21d, 22, 23 and 24) demonstrated potent dose-related TS inhibition with IC 50 values ranging from 1.57 to 3.89?M. The in vitro TS activity results were consistent with those of the cytotoxicity assay where the most potent anti-proliferative compounds of the series showed good TS inhibitory activity comparable to that of 5-fluorouracil. Furthermore, molecular docking studies were carried out to investigate the binding pattern of the designed compounds with the prospective target, TS (PDB-code: 1JU6). � 2017, Springer International Publishing AG.