Browsing by Author "El-Naggar, Mehrez E"

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    Processed Cheeses Fortified by Laurus nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model
    (NIDOC (Nat.Inform.Document.Centre), 2023-02) Hussein, Jihan; El- Bana, Mona A; Abdel Latif, Yasmin; El-sayed, Samah M; Youssef, Ahmed M; El-Naggar, Mehrez E; Medhat, Dalia
    We aimed to evaluate the protective effect of processed cheeses fortified by Laurus nobilis L. extract nanoemulsion against hyperhomocysteinemia in Ehrlich ascites carcinoma (EAC) model. Laurus nobilis L. extract nanoemulsion was prepared via spontaneous emulsification procedure. Then, nanoemulsion was added by different loadings to supplement processed cheeses. The morphology of Laurus nobilis L. extract nanoemulsion was detected using transmission electron microscopy (TEM). Female Swiss albino mice received processed cheese for two weeks then inoculated once with EAC cells. After the end of the experiment, blood samples were collected for determination of serum superoxide dismutase (SOD) , thiobarbituric acid reactive substances (TBARS), B-cell lymphoma 2 (Bcl-2), matrix metallopeptidase-9 (MMP-9), Tumor necrosis factor-α (TNF-α), and homocysteine (Hcy). Results revealed that the formation of Laurus nobilis L. extract nanoemulsion in spherical shape in size around 50 -120 nm. Mice inoculated with EAC cells showed significant increase in serum Hcy, TNF-α, TBARS, and MMP-9 levels while there was a significant decrease in SOD activity and Bcl-2 level. Pretreatment with different concentrations of Laurus nobilis L. extract significantly attenuated oxidative stress, inflammation, and induced apoptosis compared to EAC group.
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    Prophylactic effect of probiotics fortified with Aloe vera pulp nanoemulsion against ethanol-induced gastric ulcer
    (Taylor and Francis Ltd., 08/11/2021) Hussein, Jihan; El-Bana, Mona A; El-Naggar, Mehrez E; Abdel-Latif, Yasmin; El-Sayed, Samah M; Medhat, Dalia
    The purpose of this study was designed to evaluate the protective effect of probiotics fortified with Aloe vera pulp nanoemulsion on ethanol-induced gastric ulcer (GU). Freshly harvested Aloe vera pulp nanoemulsion was prepared and subsequently inoculated with 2% of the activated yogurt starter culture of Streptococcus thermophilus and Lactobacillus delbreukii subsp. bulgaricus (1:1). Chemical composition and physicochemical characterization of yogurt and the Aloe vera pulp nanoemulsion were assessed. GU was induced by ethanol. Rats were randomly assigned into control, GU, and four prophylactic groups including probiotics fortified with Aloe vera pulp nanoemulsion in the percentage of 0%, 10%, 20%, and 30% respectively. Serum levels of paraoxynase (POX) and tissue levels of malondialdehyde (MDA), nitric oxide (NO), and catalase (CAT) activity were assessed. Serum levels of nuclear factor kappa B (NF-κB), interleukin-1beta (IL-1β), matrix metalloproteinase-9 (MMP-9), ceramide, and homocysteine (Hcy) were evaluated. Results indicated that the Aloe vera pulp nanoemulsion was appeared in spherical nano form with droplets diameter around 330 nm. Ethanol induces GU to cause a significant increase in the levels of MDA, NO, NF-κB, IL-1β, MMP-9, Hcy, and ceramide along with a significant decrease in POX and CAT activities compared to the control group (p < 0.05). Pretreatment with different concentrations of probiotics fortified with Aloe vera pulp nanoemulsion with, especially the 30% concentration, significantly reduce the oxidative stress and ameliorate the release of different inflammatory mediators suggesting it as a promising approach in the protection against GU via scavenging superoxide radicals and inhibiting the activation of the inflammatory signaling cascades. © 2021 Informa UK Limited, trading as Taylor & Francis Group.
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    Stimulatory effect of docosahexaenoic acid alone or loaded in zinc oxide or silver nanoparticles on the expression of glucose transport pathway
    (Elsevier, 5/25/2021) El-Daly, Sherien M; Medhat, Dalia; El Bana, Mona; Abdel-Latif, Yasmin; El-Naggar, Mehrez E; Omara, Enayat A; Morsy, Safaa M; Hussein, Jihan
    The role of glucose transporters (GLUTs) in diabetes mellitus has become more prominent as a possible therapeutic target. In the present study, we aimed to compare the effect of zinc oxide nanoparticles (ZnONPs), silver nanoparticles (AgNPs), and docosahexaenoic acid (DHA) alone or loaded in ZnONPs or AgNPs on insulin signaling pathway and GLUTs expression in diabetic rats. In the experimental part, rats were divided into seven groups; control, diabetic, and the other five groups were diabetic received different treatments. Fasting blood sugar (FBS), serum level of insulin, insulin resistance (IR), and serum level of phosphatidylinositol 3-kinase (PI3K) were evaluated. In addition, insulin expression in pancreatic islets was assessed by immunohistochemical analysis, and the expression of liver GLUTs 1, 2, and 4 and liver insulin receptor substrate-1 (IRS-1) was evaluated by real-time polymerase chain reactions (RT-PCR). The results of the current study showed that ZnONPs, AgNPs, and DHA alone or loaded in ZnONPs or AgNPs attenuated levels of FBS, insulin and decreased IR in diabetic rats through enhancing the expression of GLUTs as well as IRS-1 and PI3K. Furthermore, AgNPs loaded with DHA showed the most significance with high comparability to the control group. In conclusion, this study elucidated the role of GLUTs and IRS-1 in diabetes and introduced novel characteristics of ZnONPs, AgNPs, and DHA alone or loaded in ZnONPs or AgNPs as a therapeutic modality to activate GLUTs and IRS1, which may be beneficial for diabetic patients with IR. Abbreviations GLUTsGlucose transportersZnONPsZinc oxide nanoparticlesAgNPsSilver nanoparticlesDHADocosahexaenoic acidFBSFasting blood sugarIRInsulin resistancePI3KPhosphatidylinositol 3-kinaseIRS-1Insulin receptor substrate-1Akt1Phosphoinositide-dependent kinase-1PKCProtein kinase CFAsFatty acidsLCPUFALong-chain polyunsaturated fatty acidsEPAEicosapentaenoic acidNPsNanoparticlesAgNO3Silver nitrateZn(NO3)26H2OZinc nitrateSTZStreptozotocinCNCCellulose nanocrystalNaOHSodium hydroxide