Browsing by Author "El-Fishawy A.M."

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    Antioxidant activity and molecular docking study of Erythrin neillii polyphenolics
    (Elsevier B.V., 2019) Gabr S.K.; Bakr R.O.; Mostafa E.S.; El-Fishawy A.M.; El-Alfy T.S.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt
    Species of genus Erythrina have a great contribution in folk medicine; various species are utilized as a tranquilizer, to treat insomnia, inflammation and colic. Besides, Erythrina species have reported antioxidant, hepatoprotective and anxiolytic activities. Erythrina neillii is a hybrid obtained through a cross between E. herbacea L. and E. humeana Spreng. It has not been well-studied for its chemical or biological profile; therefore it represents an interesting field of study. In this study, seven phenolic compounds; two hydrolysable tannins (1,3), one phenolic acid (2) and four known flavonoids (4�7) were isolated and characterized for the first time in E neillii and Erythrina genus except for vitexin (7). Isolated compounds were assessed for their antioxidant activities using ORAC assay. 2?-O-galloyl orientin (6) exhibited the highest activity followed by 2?-O-galloyl vitexin (5). Flexible molecular docking on heme oxygenase, an important stress protein that is involved in cellular protection, antioxidant and anti-inflammatory activities, justified the antioxidant activity of the isolated compounds. The best scoring was observed with 2?-O-galloyl orientin forming four binding interactions with residues, Arg 136 (two interactions), Met34 and Gly139. Erythrina neillii offered powerful and available antioxidant beside significantly active phytoconstituents. 2018 South African Association of Botanists
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    Botanical and genetic characters of Erythrina neillii cultivated in Egypt
    (Sociedade Brasileira de Farmacognosia, 2017) Gabr S.K.; Bakr R.O.; Elshishtawy H.M.; El-Fishawy A.M.; El-Alfy T.S.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; Egypt; Microbial Molecular Biology Department; Agricultural Genetic Engineering Research Institute; Giza; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
    Erythrina neillii Mabberley & Lorence, Fabaceae, is a sterile hybrid between E. herbacea L. and E. humeana Spreng. Nothing was traced about its genetic, macro and micromorphology. Therefore, it was deemed of interest to study its botanical characters, in addition to the DNA fingerprint to help in the identification of the plant. The anatomical characters of the old stem and its bark are characterized by the presence of cork cells, bast fibers and sclereids. Pericycle is sclerenchymatous forming crystal sheath. The epidermises of the leaf and young stem are characterized by the presence of anomocytic and paracytic stomata, non-glandular, unicellular and multicellular two armed hairs, and glandular club shaped hair. Calcium oxalate is present in the form of crystal sheath and prisms. Secretory cavities are distributed in the phloem and cortex. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used as one of the molecular methods to differentiate between the samples of Erythrina. The DNA of Erythrina was extracted and analyzed using seven-mer random primers. Randomly Amplified Polymorphic DNA were recognized. This characterization allows certification of the authenticity of Erythrina � neillii, in order to provide quality control for the plant. � 2017 Sociedade Brasileira de Farmacognosia.
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    Hepatoprotective activity of Erythrina neillii leaf extract and characterization of its phytoconstituents
    (Elsevier GmbH, 2019) Bakr R.O.; Fayed M.A.A.; Fayez A.M.; Gabr S.K.; El-Fishawy A.M.; Taha S.El-Alfy; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; El-Sadat City University; Egypt; Department of Pharmacology; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt
    Background: Natural antioxidants and anti-inflammatory agents have the ability to restore normal balance to destructed liver cells. The genus Erythrina has attracted attention for its broad spectrum of physiological activities and its rich polyphenolic and alkaloid contents. Hypothesis/Purpose: The major phytoconstituents of Erythrina neillii, an ornamental coral tree and a hybrid between E. herbacea and E. humeana that was not previously studied, were investigated. The hepatoprotective effect and underlying mechanisms were also assessed. Study design and methods: The main phytoconstituents in the different fractions of the alcoholic leaf extract (dichloromethane and ethyl acetate) were identified using high resolution high-performance liquid chromatography coupled with mass spectrometry (HR-HPLC-MS-MS) based on the fragmentation pattern and molecular formula of the identified compounds and on previous literature. In addition, the hepatoprotective, anti-inflammatory and antioxidant activities of three doses of E. neillii alcoholic leaf extract (100, 250, 500 mg/kg) were investigated in methotrexate (MTX)-intoxicated rats and were compared with those of silymarin-treated rats. Liver function parameters were obtained, and a histopathological study was performed. In addition, the anti-inflammatory mediators and the antioxidant system in the liver tissues were assessed. Results: The dichloromethane extract revealed an abundance of alkaloids (25), in addition to tentatively identifying flavone (1), flavanone (1) and three fatty acids. Additionally, thirty-six compounds belonging to different classes of phytoconstituents with a predominance of flavonoids (21), O/C-flavone and flavonol glycosides, followed by alkaloids (9), fatty acids (4) and (2), and phenolic glycoside were identified in the ethyl acetate extract. Compared with MTX, alcoholic leaf extract (500 mg/kg) ameliorated the MTX-induced alterations by improving several biochemical marker levels, fighting oxidative stress in serum and liver tissues, and decreasing inflammatory mediators; this finding was further confirmed by the histopathological study. Conclusion: This study reveals E. neillii, a rich source of flavonoids and alkaloids, which could be further exploited to provide a promising and safe antihepatotoxic agent source. 2018 Elsevier GmbH