Browsing by Author "El-Fishawy, A.M"

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    In-vivo wound healing activity of a novel composite sponge loaded with mucilage and lipoidal matter of Hibiscus species
    (Elsevier Masson s.r.l, 2021-03) R.O, Bakr; Amer, R.I; Attia, D; Abdelhafez, M.M; Al-Mokaddem, A.K; El Gendy, A.N; El-Fishawy, A.M; Fayed, M.A.A; Gad, S.S
    Many researches have been undergone to hasten the natural wound healing process. In this study, several Hibiscus species (leaves) were extracted with petroleum ether, methanol, and their mucilage was separated. All the tested species extracts were assessed for their viability percentage using the water-soluble tetrazolium. H.syriacus was the plant of choice to be incorporated in a new drug delivery system and evaluated for its wound healing activity. H.syriacus petroleum ether extract (PEE) showed a high percentage of palmitic and oleic acids while its mucilage demonstrated high glucosamine and galacturonic acid. It was selected to be formulated and pharmaceutically evaluated into three different composite sponges using chitosan in various ratios. Fourier-transformed infrared spectroscopy investigated the chemical interaction between the utilized sponges’ ingredients. Morphological characteristics were evaluated using scanning electron microscopy. H.syriacus composite sponge of mucilage: chitosan (1:5) was loaded with three different concentrations of PEE. Medicated formulations were assessed in rat model of excision wound model. The wound healing ability was clearly proved by the clinical acceleration, histopathological examination, and modulation of correlated inflammatory parameters as tumor necrosis factor in addition to vascular endothelial growth factor suggesting a promising valuable candidate that supports the management of excision wounds using single-dose preparation. © 2021 The Author(s)
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    The metabolomic analysis of fiveMenthaspecies: cytotoxicity, anti-Helicobacterassessment, and the development of polymeric micelles for enhancing the anti-Helicobacteractivity
    (Royal Society of Chemistry, 02/12/2021) Bakr, R.O; Tawfike, A; El-Gizawy, H.A; Tawfik, N; Abdelmohsen, U.R; Abdelwahab, M.F; Alshareef, W.A; Fayez, S.M; El-Mancy, S.M.S; El-Fishawy, A.M; Abdelkawy, M.A; Fayed, M.A.A
    Menthaspecies are medicinally used worldwide and remain attractive for research due to the diversity of their phytoconstituents and large therapeutic indices for various ailments. This study used the metabolomics examination of fiveMenthaspecies (M. suaveolens,M. sylvestris,M. piperita,M. longifolia, andM. viridis) to justify their cytotoxicity and their anti-Helicobactereffects. The activities of species were correlated with their phytochemical profiles by orthogonal partial least square discriminant analysis (OPLS-DA). Tentatively characterized phytoconstituents using liquid chromatography high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS) included 49 compounds: 14 flavonoids, 10 caffeic acid esters, 7 phenolic acids, and other constituents.M. piperitashowed the highest cytotoxicity to HepG2 (human hepatoma), MCF-7 (human breast adenocarcinoma), and CACO2 (human colon adenocarcinoma) cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays. OPLS-DA and dereplication studies predicted that the cytotoxic activity was related to benzyl glucopyranoside-sulfate, a lignin glycoside. Furthermore,M. viridiswas effective in suppressing the growth ofHelicobacter pyloriat a concentration of 50 mg mL−1. OPLS-DA predicted that this activity was related to a dihydroxytrimethoxyflavone.M. viridisextract was formulated with Pluronic® F127 to develop polymeric micelles as a nanocarrier that enhanced the anti-Helicobacteractivity of the extract and provided minimum inhibitory concentrations and minimum bactericidal concentrations of 6.5 and 50 mg mL−1, respectively. This activity was also correlated to tentatively identified constituents, including rosmarinic acid, catechins, carvone, and piperitone oxide. © The Royal Society of Chemistry 2021