Browsing by Author "El-Deib K.M."

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Pomegranate peel attenuates aluminum-induced hepatorenal toxicity
(2013) Abdel Moneim A.E.; Othman M.S.; Mohmoud S.M.; El-Deib K.M.; Department of Zoology and Entomology; Faculty of Science; Helwan University; 11795 Helwan; Cairo; Egypt; Biochemistry and Molecular Biology Department; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); Giza; Egypt; Department of Zoology; Faculty of Science; Cairo University; Cairo; Egypt; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza; Egypt
The present study was undertaken to determine the potential role of methanolic extract of pomegranate peel (MEPP) in modulating aluminum chloride (AlCl3) induced hepatorenal toxicity in female rats. The effect of MEPP (200mg/kgbwt) on AlCl3 (34mg/kgbwt) induced hepatorenal toxicity, accumulation of aluminum (Al), hepatorenal functions and oxidant/antioxidant status of liver and kidney were determined. The changes of liver and kidney structures were investigated with hematoxyline and eosin, in addition, the anti-apoptotic effect of MEPP was analyzed by immunohistochemistry. The present study showed an indication of carcinogenicity in the AlCl3 treated group represented by an increase in tumor necrosis factor-? and angiogenin and inflammation by inducing an increase in prostaglandin E2 and F2?. MEPP protected liver and kidney through reduce the Al accumulation, stimulated antioxidant activities and elevated the anti-apoptotic protein namely Bcl-2. Therefore, these results indicated that the methanolic extract of pomegranate peel has beneficial influences and could be able to inhibit Al-induced oxidative stress and histopathological alternations in liver and kidney of female rats, and these effects may be related to anti-apoptotic and antioxidant activities. � 2013 Informa Healthcare USA, Inc.
The potential role of Azadirachta indica treatment on cisplatin-induced hepatotoxicity and oxidative stress in female rats
(2013) Dkhil M.A.; Al-Quraishy S.; Aref A.M.; Othman M.S.; El-Deib K.M.; Abdel Moneim A.E.; Department of Zoology; College of Science; King Saud University; Riyadh 11451; Saudi Arabia; Department of Zoology and Entomology; Faculty of Science; Helwan University; Cairo 11795; Egypt; Biological Science Department; Faculty of Dentistry; Modern Science and Arts University (MSA); Giza 12111; Egypt; Biochemistry and Molecular Biology Department; Faculty of Pharmacy; Modern Science and Arts (MSA); Giza 12111; Egypt; Molecular Drug Evaluation Department; National Organization for Drug Control and Research (NODCAR); Giza 12553; Egypt; Department of Biochemistry and Molecular Biology; Asturias Institute of Biotechnology; University of Oviedo; Oviedo 33006; Spain
Azadirachta indica A. Juss. (neem, family: Meliaceae) is perhaps the most commonly used traditional medicinal plant of India. In this study we investigated the protective effect of methanolic neem leaves extract (MNLE; 500 mg/Kg bwt) on rats treated with cisplatin (CDDP)-induced hepatotoxicity. Adult rats were randomly divided into four groups. CDDP was given to rats by intraperitoneal injection, while MNLE was given by oral gavage for 5 days after the CDDP injection. The injury and oxidative stress caused by CDDP on the liver and the effect of MNLE were evaluated by measuring (a) histological changes, (b) tissue biochemical oxidant and antioxidant parameters, and (c) investigating apoptosis markers immunohistochemically and by real time PCR. After treatment with MNLE, the histological damage and apoptosis induction caused by cisplatin were improved. Malondialdehyde and nitric oxide were significantly decreased; the antioxidant system, namely, glutathione content, glutathione-S-transferase, glutathione peroxidase, catalase, and superoxide dismutase activities were significantly elevated. In conclusion, MNLE may have a potential role when combined with cisplatin in chemotherapy to alleviate cisplatin-induced damage and oxidative stress in liver. 2013 Mohamed A. Dkhil et al.