Browsing by Author "El-Bishbishy, Mahitab H"

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    Comparative Botanical and Genetic Diversity Study of Different Cultivars of Sesamum indicum L. Cultivated in Egypt
    (Natural Product Research Group University of Benin, 11/02/2020) El-Hawary, Seham S; Ashour, Rehab M. S; El-Bishbishy, Mahitab H; Okba, Mona M; Hassan, Heba A
    Sesame (Sesamum indicum L.) is a plant belonging to the Family Pedaliaceae. It grows worldwide in India, Sudan and China. The high nutritive and curative effects of sesame favour its wide use in culinary and traditional medicines in many countries. No reports could be traced concerning the macromorphological and micromorphological characterization or genetic profiling of S. indicum L. cultivars (Shandawel-3, Giza-32 and Toshka) cultivated in Egypt. The establishment of proper identification and authentication of such valuable plant deemed necessary. Therefore, botanical and genetic diversity study of the three cultivars using RAPD markers were investigated. Detailed morphological study through examination of transverse sections in the lamina and midrib region, examination of surface preparations and powders of the three cultivars.Also, Genetic study was performed using twelve primers producing a total of ninety-nine RAPD fragments, of which twenty-five bands (25.25%) were polymorphic. The number of bands per primer ranged from two to fourteen, while the number of polymorphic bands ranged from zero to six and monomorphic bands ranged from two to twelve. Unique bands were observed with eleven primers. Unweighted pair group method using arithmetic average (UPGMA) clustering resulted in two major groups. Results showed that all cultivars have nearly similar macro and micromorphological characters but differ in the dimensions of certain elements with high level of genetic similarity. Thus, this study helped in providing a useful tool for identification of sesame for germplasm banks maintenance and effective parents selection in breeding programs.
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    The pulmonary protective potential of vanillic acid-loaded TPGS-liposomes: modulation of miR-217/MAPK/NF-κb signalling pathway
    (Taylor and Francis Ltd, 2024-04) Sweed, Nabila M; Zaafan, Mai A; El-Bishbishy, Mahitab H; Dawoud, Marwa H.S
    The aim is to investigate the possible pulmonary protective effect of vanillic acid (VA) in liposome-TPGS nanoparticles, to overcome VA’s poor bioavailability. VA was successfully extracted. Liposomes were prepared using thin film hydration. Central composite design was adopted for optimisation of liposomes to get the maximum entrapment efficiency (EE%) and the minimum mean diameter, where the liposomes were further modified with TPGS, and tested for PDI, zeta-potential, and in-vitro drug release. In-vivo study on mice with LPS-acute pulmonary toxicity was tested. TPGS-modified VA-liposomes showed EE% of 69.35 ± 1.23%, PS of 201.7 ± 3.23 nm, PDI of 0.19 ± 0.02, and zeta-potential of −32.2 ± 0.32 mv. A sustained drug release of the TPGS-modified VA-liposomes was observed compared to standard VA, and a pulmonary-protective effect through decreasing miR-217 expression with subsequent anti-inflammatory effect through suppression of MAPK and PI3K/NF-κB pathways was also demonstrated in the current study. TPGS-modified VA-liposomes showed an enhanced bioavailability and a sustained drug release with promising pulmonary protective effects against acute pulmonary injury diseases.