Browsing by Author "El-Alfy T.S."

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    Antioxidant activity and molecular docking study of Erythrin neillii polyphenolics
    (Elsevier B.V., 2019) Gabr S.K.; Bakr R.O.; Mostafa E.S.; El-Fishawy A.M.; El-Alfy T.S.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; 11787; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; 11562; Egypt
    Species of genus Erythrina have a great contribution in folk medicine; various species are utilized as a tranquilizer, to treat insomnia, inflammation and colic. Besides, Erythrina species have reported antioxidant, hepatoprotective and anxiolytic activities. Erythrina neillii is a hybrid obtained through a cross between E. herbacea L. and E. humeana Spreng. It has not been well-studied for its chemical or biological profile; therefore it represents an interesting field of study. In this study, seven phenolic compounds; two hydrolysable tannins (1,3), one phenolic acid (2) and four known flavonoids (4�7) were isolated and characterized for the first time in E neillii and Erythrina genus except for vitexin (7). Isolated compounds were assessed for their antioxidant activities using ORAC assay. 2?-O-galloyl orientin (6) exhibited the highest activity followed by 2?-O-galloyl vitexin (5). Flexible molecular docking on heme oxygenase, an important stress protein that is involved in cellular protection, antioxidant and anti-inflammatory activities, justified the antioxidant activity of the isolated compounds. The best scoring was observed with 2?-O-galloyl orientin forming four binding interactions with residues, Arg 136 (two interactions), Met34 and Gly139. Erythrina neillii offered powerful and available antioxidant beside significantly active phytoconstituents. 2018 South African Association of Botanists
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    Botanical and genetic characters of Erythrina neillii cultivated in Egypt
    (Sociedade Brasileira de Farmacognosia, 2017) Gabr S.K.; Bakr R.O.; Elshishtawy H.M.; El-Fishawy A.M.; El-Alfy T.S.; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Sciences and Arts; Giza; Egypt; Microbial Molecular Biology Department; Agricultural Genetic Engineering Research Institute; Giza; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
    Erythrina neillii Mabberley & Lorence, Fabaceae, is a sterile hybrid between E. herbacea L. and E. humeana Spreng. Nothing was traced about its genetic, macro and micromorphology. Therefore, it was deemed of interest to study its botanical characters, in addition to the DNA fingerprint to help in the identification of the plant. The anatomical characters of the old stem and its bark are characterized by the presence of cork cells, bast fibers and sclereids. Pericycle is sclerenchymatous forming crystal sheath. The epidermises of the leaf and young stem are characterized by the presence of anomocytic and paracytic stomata, non-glandular, unicellular and multicellular two armed hairs, and glandular club shaped hair. Calcium oxalate is present in the form of crystal sheath and prisms. Secretory cavities are distributed in the phloem and cortex. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used as one of the molecular methods to differentiate between the samples of Erythrina. The DNA of Erythrina was extracted and analyzed using seven-mer random primers. Randomly Amplified Polymorphic DNA were recognized. This characterization allows certification of the authenticity of Erythrina � neillii, in order to provide quality control for the plant. � 2017 Sociedade Brasileira de Farmacognosia.